Journal of the Korea Institute of Information and Communication Engineering (한국정보통신학회논문지)

The Korea Institute of Information and Commucation Engineering (한국정보통신학회)
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3D sensing and segmentation of microorganism using microfluidic device and digital holography

미세유체소자와 디지털 홀로그래피 기술을 이용한 미생물의 3D 이미징과 세그먼테이션

  • 신동학 (동서대학교 IAI연구소) ;
  • 이준재 (계명대학교 게임모바일콘텐츠학과)
  • Received : 2012.09.18
  • Accepted : 2012.10.25
  • Published : 2013.02.28
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Abstract

Microfluidic devices can offer precise control for a verity of tasks involving biological specimen. In this paper, we propose an integrated system consisting of a microfluidic device along with a digital holographic microscope and present three-dimensional (3D) sensing and segmentation of biological microorganisms. When the individual microorganisms are inputted into the microfluidic channel, the holographic microscope records their holograms. The holograms are computationally reconstructed in 3D using Fresnel transform and the reconstructed phase images are used to search the position of microorganisms. Optical experiments are carried out and experimental results are presented to illustrate the usefulness of the proposed system.

미세유체소자(microfluidic device)는 미생물과 관련된 다양한 작업들에 대해서 정확한 제어를 제공할 수 있다. 본 논문에서는 미세유체 소자와 디지털 홀로그래피 마이크로스코피 기술로 구성된 시스템을 구성하고 미생물의 3D 이미징과 세그먼테이션을 설명한다. 각각의 미생물은 미세유체 채널을 통하여 흘러가며 홀로그래피 마이크로스코피가 홀로그램을 기록한다. 기록된 홀로그램은 Fresnel 변환을 통하여 컴퓨터적으로 복원되며, 복원된 영상의 위상성분을 이용하여 미생물의 위치 정보를 찾기 위한 세그먼테이션을 수행한다. 제안하는 방법의 유용함을 설명하기 위하여 광학 실험을 수행하고 그 결과를 나타내었다.

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