Journal of the Korean Crystal Growth and Crystal Technology (한국결정성장학회지)

The Korea Association of Crystal Growth (한국결정성장학회)
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Development and application of the magnetic field device for single crystal growth

자기장 단결정 성장장치의 개발과 적용

  • Kim, Shin Ae (Neutron Science Division, Korea Atomic Energy Research Institute) ;
  • Cho, Sang-Jin (Neutron Science Division, Korea Atomic Energy Research Institute) ;
  • Magay, Elena (Neutron Science Division, Korea Atomic Energy Research Institute) ;
  • Ryu, Sun-Young (Neutron Science Division, Korea Atomic Energy Research Institute)
  • 김신애 (한국원자력연구원 중성자과학연구부) ;
  • 조상진 (한국원자력연구원 중성자과학연구부) ;
  • 엘레나 마가이 (한국원자력연구원 중성자과학연구부) ;
  • 류선영 (한국원자력연구원 중성자과학연구부)
  • Received : 2012.11.29
  • Accepted : 2013.02.08
  • Published : 2013.02.28
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Abstract

The three-dimensional structure of the proteins, including the location of the hydrogen atoms can be analyzed by using neutron diffraction. In order to grow single crystals for neutron diffraction experiments, we developed a simple magnetic field device with the commercial magnets and succeeded in growing hen egg-white lysozyme single crystals of more than 1 $mm^3$ in volume using this device. The crystals grown with a magnetic field were larger and had perfect transparency, whereas the crystals grown without a magnetic field had micro-cracks. The result of the X-ray measurement showed a good resolution and small mosaicity for the crystals grown in the presence of a magnetic field.

중성자회절을 이용하여 단백질 내의 수소원자 위치를 포함한 3차원 상세구조를 분석할 수 있으므로, 중성자 회절용 단백질 단결정을 성장시키기 위해 상용자석을 이용한 자기장 단결정 성장장치를 개발하였다. 이 장치를 이용하여 중성자 회절실험에 필요한 1 $mm^3$ 이상 부피의 lysozyme 단결정 시료를 성장시켰다. 자기장 영향 하에서 성장시킨 단결정은 자기장의 영향 없이 성장한 시료보다 평균 부피가 크고 결정도가 우수하였으며, X-선 측정 결과에서도 해상도가 높고 작은 mosaicity를 나타내었다.

Keywords

References

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