Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Phenolic Compound Content and Antioxidant Activity of Citrus Peels

감귤 과피의 페놀성 화합물 함량과 항산화 활성

  • 황준호 (제주대학교 생물학과) ;
  • 박경열 (제주대학교 식품생명공학과) ;
  • 오유성 (제주대학교 생물학과) ;
  • 임상빈 (제주대학교 생명과학기술혁신센터)
  • Received : 2012.09.17
  • Accepted : 2012.11.14
  • Published : 2013.02.28
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Abstract

The peel from seven types of citrus was extracted with 80% methanol, and their phenolic compound content, oxygen radical absorbance capacity (ORAC), inhibitory activities of nitric oxide (NO), and reactive oxygen species (ROS) production induced by LPS and t-BHP in LPS-activated RAW 264.7 cells were measured. Total phenolic content was high in Yungkyool, Cheonhyehyang, and Jinkyool (30.6, 30.2, and 28.2 mg GAE/g, respectively), while total flavonoid content was high in Yungkyool and Jinkyool (30.3 and 25.5 mg RE/g, respectively). ORAC was the highest at 1,076 mM TE/g in Yungkyool, followed by Cheonhyehyang (1,012), Jinkyool (984), and Hallabong (914). High inhibitory activity against NO production was shown in Cheonhyehyang, Yungkyool, and Jinkyool with $IC_{50}$ values of 215.3, 259.2, and 328.9 ${\mu}g/mL$, respectively. LPS-induced ROS production was inhibited by 16.4% and 12.8% in Hallabong and Jinkyool, while t-BHP-induced ROS production was inhibited by 28.7%, 26.1%, and 26.6% in Jinkyool, Hallabong, and Cheonhyehyang, respectively. Correlation coefficients between total phenolic, total flavonoid content, and ORAC were 0.884 and 0.855. Inhibitory activity against NO production showed higher correlation with total phenolic content than total flavonoid content. It was concluded that citrus peels had potent antioxidant activities and could be used as natural antioxidants in the food and pharmaceutical industries.

감귤 7종을 대상으로 과피를 80% 메탄올로 추출하여 총페놀과 총플라보노이드 함량을 측정하였고, 유해산소 흡수능력(ORAC) 분석을 통한 free radical 소거능, RAW 264.7 murine macrophage를 이용한 NO 생성 저해능, LPS와 t-BHP 자극에 의한 활성산소 생성 저해 활성을 분석하였다. 총페놀 함량은 영귤, 천혜향, 진귤이 각각 30.6, 30.2, 28.2 mg GAE/g으로 높은 값을 나타내었고, 총플라보노이드 함량은 영귤과 진귤이 각각 30.3와 25.5 mg RE/g으로 높은 값을 나타내었다. ORAC 분석을 통한 free radical 소거능은 영귤이 1076 mM TE/g으로 가장 높은 활성을 나타내었으며, 그 다음이 천혜향(1012), 진귤(984), 한라봉(914) 순이었다. 마우스 대식세포인 RAW 264.7에 의한 NO 생성 저해능 ($IC_{50}$)은 천혜향이 215.3 ${\mu}g/mL$로 가장 높았으며, 그 다음으로 영귤(259.2), 진귤(328.9) 순이었다. LPS 자극에 의한 활성산소 생성은 한라봉이 16.4%, 진귤이 12.8% 저해하였고, t-BHP 자극에 의한 활성산소 생성은 진귤, 한라봉, 천혜향이 각각 28.7, 26.1, 26.6% 저해하였다. 총페놀과 ORAC 간의 상관계수는 0.884이고 총플라보노이드와 ORAC 간의 상관계수는 0.855로, ORAC에 의한 항산화 활성은 총페놀 및 총플라보노이드의 함량과 밀접한 관계가 있는 것으로 나타났다. 총페놀 및 총플라보노이드 함량과 NO 생성 저해능 간의 상관계수는 각각 0.921과 0.683으로 NO 생성 저해능도 총페놀 및 총플라보노이드 함량과 밀접한 관계가 있었다.

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References

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