Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Biocontrol of Biofilm-forming Bacillus cereus by Using Organic Acid, Ethanol, and Sodium Chloride

생물막 형성 Bacillus cereus에 대한 유기산, 에탄올 및 NaCl의 제어효과

  • Lee, Young-Duck (Department of Food Science and Biotechnology, Gachon University) ;
  • Yoo, Hye-Lim (Department of Food Science and Biotechnology, Gachon University) ;
  • Park, Jong-Hyun (Department of Food Science and Biotechnology, Gachon University)
  • 이영덕 (가천대학교 식품생물공학과) ;
  • 유혜림 (가천대학교 식품생물공학과) ;
  • 박종현 (가천대학교 식품생물공학과)
  • Received : 2012.10.10
  • Accepted : 2012.10.24
  • Published : 2013.02.28
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Abstract

Food poisoning by Bacillus cereus is one of the common food-borne diseases and B. cereus is widely distributed in natural and commercial products owing to the strong resistance caused by biofilm or spore. The ethanol, NaCl, and organic acids of acetic acid, citric acid, and lactic acid for biocontrol of biofilm-forming B. cereus on glass wool were investigated. The biofilm on glass wool was observed in many developments after 48 h incubation. As the results of reduction of biofilm-forming B. cereus by sanitizers, reduction levels of each organic acid treatment ranged to 5-6 log CFU/g-glass wool. In case of combination treatments of 20% ethanol, 10% NaCl, and 1% of each organic acid for 1-5 min, the reduction level of biofilm-forming B. cereus was 7-8 log CFU/g-glass wool. Therefore, combination treatments of ethanol, NaCl, and an organic acid might effectively reduce biofilm-forming B. cereus in various food processes and industries.

유리섬유를 이용하여 B. cereus의 biofilm 형성 조건을 확인한 결과 3시간 이후부터 부착하여 48시간에 biofilm이 많이 형성된 것을 확인하였다. 그리고 biofilm 형성 B. cereus의 제어를 위해 에탄올, NaCl, 유기산을 각각 단독으로 처리하였을 때 1% 젖산, 아세트산, 구연산을 30분 처리하였을 때 약 5-6 log CFU/g-glass wool 수준 감소하여 가장 좋은 효과를 나타냈다. 또한, 20% 에탄올과 각각의 1% 유기산, 20% 에탄올과 10% NaCl의 두 가지를 혼합 처리의 경우 단독으로 처리했을 때보다 저감화 효과가 우수했으며, 20% 에탄올과 10% NaCl 및 각각의 1% 유기산을 1-5분 동안 혼합 처리 했을 때 약 7-8 log CFU/g-glass wool 수준이 감소하여 biofilm 형성 B. cereus의 제어에 가장 효과적이었다. 따라서 본 연구 결과를 통해 유기산, 에탄올, NaCl 복합 처리 방법을 사용하여 biofilm 형성 B. cereus의 저감화를 효과적으로 할 수 있을 것으로 판단된다.

Keywords

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