Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Comparison of Conventional Culture Method and Real-time PCR for Detection of Yersinia enterocolitica in Sausage and Vegetable Salad

소시지와 야채 샐러드에서 Yersinia enterocolitica 검출을 위한 배지법과 real-time PCR법의 비교

  • Kim, Yun-Gyeong (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University) ;
  • Chon, Jung-Whan (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University) ;
  • Lee, Jae-Hoon (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University) ;
  • Kwak, Hyo-Sun (Division of Foodborne Diseases Prevention and Surveillance, National Institute of Food and Drug Safety Evaluation) ;
  • Hwang, In-Gyun (Division of Food Chemical Residues, National Institute of Food and Drug Safety Evaluation) ;
  • Seo, Kun-Ho (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University)
  • 김윤경 (건국대학교 KU 식품안전연구소) ;
  • 천정환 (건국대학교 KU 식품안전연구소) ;
  • 이재훈 (건국대학교 KU 식품안전연구소) ;
  • 곽효선 (식품의약품 안전평가원 식중독 예방관리과) ;
  • 황인균 (식품의약품 안전평가원 화학물질과) ;
  • 서건호 (건국대학교 KU 식품안전연구소)
  • Received : 2012.08.07
  • Accepted : 2012.09.24
  • Published : 2013.02.28
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Abstract

The purpose of this study was to compare a conventional culture method and real-time PCR for the detection of Yersinia enterocolitica (Y. enterocolitica) in sausage and in vegetable salad. Food samples inoculated with Y. enterocolitica were enriched in peptone-sorbitol bile-broth, and swabs were then streaked onto cefsulodin-irgasan-novobiocin agar. Biochemical tests for suspected colonies were performed with an API 20E strip. In parallel, real-time PCR was performed, targeting the 16S rRNA gene using 1 mL of enrichment broth. In sausage, the number of positive samples detected by culture method (49 out of 60) was similar (p>0.05) with that of real-time PCR (50 out of 60). However, the number of positive samples of real-time PCR (26 out of 60) was significantly higher (p<0.05) than that of the conventional culture method (6 out of 60) in vegetable salad. Real-time PCR could be an effective screening tool for detecting Y. enterocolitica, particularly in food samples with high levels of background flora, such as a vegetable salad.

본 연구에서는 소시지와 야채 샐러드에서 Y. enterocolitica의 검출을 위해 배지법과 real-time PCR법을 비교하였다. 소시지와 야채 샐러드에 Y. enterocolitica를 접종하고 PSBB에서 증균배양 하였으며, CIN agar에서 선택배양하였다. 동시에 증균배양액에서 1 mL을 채취하여 real-time PCR을 실시하였다. 실험결과, real-time PCR은 소시지에서 배지검출법과 비교하여 동일한 검출력을 보였으나 야채 샐러드에서는 훨씬 더 많은 양성을 검출하였다(p<0.05). 결론적으로 real-time PCR은 식품 시료와 관계 없이 표준 검출법인 배지검출법과 비교하여 동등하거나 우수한 민감도를 지닌 신속검출기법인 것으로 사료되며, 배지검출법에 앞서 선별검사로 사용할 경우 시간, 비용, 노동력 절감에 있어서 매우 유효한 방법이 될 것으로 판단된다.

Keywords

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