Identification and Analysis of the Novel pGAPDH-w Gene Differentially Expressed in Wild Ginseng

  • Han, Young-Ju (Graduate School of Oriental Medicine, Sangji University) ;
  • Kwon, Ki-Rok (Research Center of the Korean Pharmacopuncture Institute) ;
  • Kang, Won-Mo (Department of Biochemistry, Graduate School of Medicine, Inha University) ;
  • Jeon, Eun-Yi (Department of Biochemistry, Graduate School of Medicine, Inha University) ;
  • Jang, Jun-Hyeog (Department of Biochemistry, Graduate School of Medicine, Inha University)
  • 투고 : 2012.04.04
  • 심사 : 2012.10.31
  • 발행 : 2013.03.31


Objective: Panax ginseng is one of the most medicinally used herbal medicines in the world. Wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the differences between wild ginseng and cultivated ginseng. Method: To identify wild ginseng-specific genes, we used suppressive subtraction hybridization. Results: We report that one of the clones isolated in this screen was the GAPDH (glyceraldehyde 3-phosphate dehydrogenase) gene (designated pGAPDH-w). DNA BLAST sequence analysis revealed that this pGAPDH-w gene contained novel sequences of 94 bp. RT-PCR results showed that the expression of the pGAPDH-w gene was significantly up-regulated in the wild ginseng as compared with the cultivated ginseng. Conclusion: The pGAPDH-w gene may be one of the important markers of wild ginseng.


연구 과제 주관 기관 : Ministry for Agriculture, Forestry and Fisheries


  1. Kwon KR, Owi JS, Kim SW. [Literature study of wild ginseng]. Pharmacopuncture. 2003;6(2):67-75. Korean.
  2. Wang CZ, Mehendale SR, Yuan CS. Commonly used antioxidant botanicals: active constituents and their potential role in cardiovascular illness. Am J Chin Med. 2007;35(4):543-58.
  3. Tachikawa E, Kudo K, Hasegawa H, Kashimoto T, Sasaki K, Miyazaki M, et al. In vitro inhibation of adrenal catecholamine secretion by steroidal metabolites of ginseng saponins. Biochem Pharmacol. 2003;66(11):2213-21.
  4. Lee Y, Jin Y, Lim W, Ji S, Choi S, Jang S, et al. A ginsenoside-Rh1, a component of ginseng saponin, activates estrogen receptor in human breast carcinoma MCF-7 cells. J Steroid Biochem Mol Biol. 2003;84(4):463-8.
  5. Kwon KR, Seo JC. [Genetical identification of Korean wild ginseng and American wild ginseng by using pyrosequencing method]. Kor J Herbology. 2004;19(4):45-50. Korean.
  6. Kwon KR, Park WP, Kang WM, Jeon EY, Jang JH. Identification and analysis of differentially expressed genes in mountain cultivated ginseng and mountain wild ginseng. J Acupunct Meridian Stud. 2011;4(2): 123-8.
  7. Kim DY, Kwon KR, Kang WM, Jeon EY, Jang JH. Identification and expression analysis of chloroplast ppsbB gene differentially expressed in wild ginseng. Pharmacopuncture. 2012;15(1):18-22.
  8. Lee KH, Kwon KR, Kang WM, Jeon EM, Jang JH. Identification and analysis of the chloroplast rpoC1 gene different expressed in wild ginseng. Pharmacopuncture. 2012;15(2): 20-3.
  9. Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, et al. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci USA. 1996;93(12):6025-30.
  10. Diatchenko L, Lukyanov S, Lau YF, Siebert PD. Suppression subtractive hybridization: a versatile methods for identifying differentially expressed genes. Methods Enzymol. 1999;303:349-80.
  11. Lui JH, Staba EJ. The ginsenosides of various ginseng plants and selected products. J Nat Prod. 1980;43(3):340-6.
  12. Lim W, Mudge KW, Vermeylen F. Effects of population, age, and cultivation methods on ginsenoside content of wild American ginseng (Panax quinquefolium). J Agric Food Chem. 2005;53(22):8498-505.
  13. Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pharmacol. 1999;58(11):1685-93.
  14. Huang YC, Chen CT, Chen SC, Lai PH, Liang HC, Chang Y, et al. A natural compound (ginsenoside Re) isolated from Panax ginseng as a novel angiogenic agent for tissue regeneration. Pharm Res. 2005;22(4):636-46.
  15. Sengupta S, Toh SA, Sellers LA, Skepper JN, Koolwijk P, Leung HW, et al. Modulating angiogenesis: the yin and the yang in ginseng. Circulation. 2004;110(10):1219-25.
  16. Cerff R. Glyceraldehyde-3-phosphate dehydrogenase (NADP) from Sinapis alba L. NAD(P)-induced conformation changes of the enzyme. Eur J Biochem. 1978;82(1):45-53.
  17. Cerff R. Glyceraldehyde-3-phosphate dehydrogenase (NADP) from Sinapis alba L: aeversible association of the enzyme with a protein factor as controlled by pyridine nucleotides in vitro. Plant Physiol. 1978;61(3):369-72.

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