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국내 시판 경옥고 제품의 품질 특성 및 항염증 활성

Qualities and Anti-inflammatory Activity of Kyungokgos Sold in Local Markets

  • 이가순 (충남농업기술원 금산인삼약초시험장) ;
  • 김관후 (충남농업기술원 금산인삼약초시험장) ;
  • 김현호 (충남농업기술원 금산인삼약초시험장) ;
  • 성봉재 (충남농업기술원 금산인삼약초시험장) ;
  • 김선익 (충남농업기술원 금산인삼약초시험장) ;
  • 한승호 (충남농업기술원 금산인삼약초시험장) ;
  • 강은주 (건양대학교 의과대학) ;
  • 유영춘 (건양대학교 의과대학)
  • Lee, Ka-Soon (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Kim, Gwan-Hou (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Kim, Hyun-Ho (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Seong, Bong-Jae (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Kim, Sun-Ick (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Han, Seung-Ho (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES) ;
  • Kang, Eun Ju (Dept. of Microbiology, College of Medicine, Konyang University) ;
  • Yoo, Yung Choon (Dept. of Microbiology, College of Medicine, Konyang University)
  • 투고 : 2012.10.11
  • 심사 : 2013.01.29
  • 발행 : 2013.03.31

초록

시판 경옥고 제품의 품질 특성과 항염증 활성에 대한 특성을 비교 분석하였다. 총 10종에 대한 일반성분을 분석한 결과, 수분(18.62~49.78%), 탄수화물(47.95~77.08%), 단백질(0.89~3.58%), 지질(0.16~1.14%) 및 회분(0.20~1.21%)이었고, 진세노사이드는 홍삼특유 사포닌인 Rg3, Rh1, Rh2 및 F1 등의 사포닌이 검출되어 총사포닌 함량은 0.24~0.76 mg/g이었으며, 생지황의 지표물질인 catalpol의 함량은 0.12~1.10 mg/g이었다. 유리당 조성 및 함량은 glucose가 11.00~22.38%로 가장 많이 함유되어 있으며, 이어서 fructose, sorbitol, maltose, 3당류 그리고 sucrose 순으로 검출되었고 총함량은 29.09~41.34%이었다. 색도는 백색도는 26.49~73.87, 적색도는 $30{\pm}10$, 황색도는 80~90의 범위에 있었다. 경옥고 제품의 물성은 점도특성으로 볼 때 일정한 온도에서 일정 회전속도로 반복적으로 작동시킬 경우 비례적으로 점도가 증가하는 특성을 가졌으며, 본 시험에 이용된 경옥고 제품의 물성은 크게 3가지 구분으로 grouping 되었는데 정적인 상태에서 고체상을 가지는 제품은 3, 5 및 10번이었고, 제품 2, 6 및 4번은 4회 반복 시까지는 일정값으로 비례적으로 상승하다가 그 이후부터는 일정한 값을 나타냈고, 7 및 8번은 한번 회전시킨 이후부터 거의 일정한 값을 보여주는 유동적인 특성을 보여주었으며, 탄수화물의 함량이 높은 것일수록 유동성이 큰 물성을 보여주었다. 시판 경옥고 제품의 열수추출물의 처리에 의한 RAW 264.7 대식세포에의 증식에 미치는 영향은 제품마다 약간씩 차이가 남을 볼 수 있었고, 제품 중 1, 2, 4, 5, 6, 7, 8번 제품이 세포독성이 낮은 것으로 나타났으며, 특히 8번 제품은 가장 안전한 것으로 관찰되었다. LPS를 대식세포에 처리하기 전에 각 경옥고 제품의 추출물을 전처리하여 항염증 활성을 조사한 결과를 종합해 보면, 세포독성이 낮으면서 대식세포로부터의 염증 매개인자의 분비를 억제하는 항염증 활성은 1번과 7번 제품이 가장 우수하며, 2번 제품도 비교적 높은 안전성과 염증반응 억제효과를 가지는 것으로 판단되었다.

Kyungokgos purchased in local markets in Korea vary in their combination and mixing ratios during processing. This study was investigated qualities of Kyungokgos manufactured traditionally to evaluating its qualities. The general components of Kyungokgos were moisture (18.62~49.78%), ash (0.198~1.211%), protein (0.89~3.58%), lipid (0.16~1.14%) and carbohydrates (47.95~77.08%). The color values of L, a, and b were 26.49~73.87, 16.51~38.64, and 45.41~88.94, respectively. The viscosity was classified into three non-Newtonian type groups: high, medium, and non-dilatant, according to the increase of loop execution times. Three extracts (KOG-1, -7, and -8, in a 30-fold dilution) showed no cytotoxicity toward RAW 264.7 cells, while the extracts of KOG-2, -4, and -5 showed a low cytotoxic effect. KOG-1 and -2 extracts with low cytotoxicity markedly inhibited the production of the inflammatory mediators-nitric oxide (NO) and tumor necrosis factor-alpha (TNF-${\alpha}$) in LPS-stimulated RAW 264.7 cells. These results indicate that KOG-1 and -2 extracts have anti-inflammatory activity in LPS-stimulated RAW 264.7 macrophages.

키워드

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피인용 문헌

  1. LPS로 염증유도된 RAW 264.74 세포에 대한 흑삼 첨가 경옥고의 항염증 효과 vol.32, pp.3, 2013, https://doi.org/10.6116/kjh.2017.32.3.19
  2. Deciphering the Antitussive, Expectorant, and Anti-Inflammatory Potentials of ShashamKyeongok-Go and Their Phytochemical Attributes: In Vivo Appraisal in ICR Mice vol.11, pp.3, 2013, https://doi.org/10.3390/app11031349