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Pre-treatment of the White-Spotted Flower Chafer (Protaetia brevitarsis) as an Ingredient for Novel Foods

흰점박이꽃무지(Protaetia brevitarsis)의 식품원료화를 위한 전처리 조건 확립

  • Kwon, Eun-Young (Dept. of Agricultural Biology, National Academy of Agricultural Science, Rural Department Administration) ;
  • Yoo, Jeongmi (Dept. of Agricultural Biology, National Academy of Agricultural Science, Rural Department Administration) ;
  • Yoon, Young-Il (Dept. of Agricultural Biology, National Academy of Agricultural Science, Rural Department Administration) ;
  • Hwang, Jae-Sam (Dept. of Agricultural Biology, National Academy of Agricultural Science, Rural Department Administration) ;
  • Goo, Tae-Won (Dept. of Agricultural Biology, National Academy of Agricultural Science, Rural Department Administration) ;
  • Kim, Mi-Ae (Dept. of Agricultural Biology, National Academy of Agricultural Science, Rural Department Administration) ;
  • Choi, Young-Cheol (Dept. of Agricultural Biology, National Academy of Agricultural Science, Rural Department Administration) ;
  • Yun, Eun-Young (Dept. of Agricultural Biology, National Academy of Agricultural Science, Rural Department Administration)
  • 권은영 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 유정미 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 윤영일 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 황재삼 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 구태원 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김미애 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 최영철 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 윤은영 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2012.10.09
  • Accepted : 2013.01.17
  • Published : 2013.03.31

Abstract

The pharmacological efficacy of Protaetia (P.) brevitarsis larvae has been described in the Dongui Bogam. It is believed that the larvae are particularly useful for hepatic disorders. However, natural aversion has made it difficult to consume these larvae as food. Thus, we sought to make an eatable form of the larvae by establishing optimal conditions for larvae preparation. Larvae were selectively bred, sterilized, and a powder of larvae generated by freeze-drying. Afterward, the CellTiter $96^{(R)}$ AQueous Non-Radioactive Cell Proliferation Assay (MTS) with the RAW 264.7 cell line was used to validate the safety of the powder as a food ingredient. We determined that oak sawdust sterilized by water vapor for 5 minutes could be used for larvae feed, and a feeding for 3~5 days followed by a fasting for 3 days were optimal conditions for larvae preparation. In addition, sterilization of larvae at $115^{\circ}C$ and $0.9kgf/cm^3$ (to avoid contamination of pathogenic bacteria and fungi) was successfully applied in the production of edible powder from P. brevitarsis. The optimized processes established in our experiments can be used in the industrial production of P. brevitarsis as a food ingredient.

본 연구에서는 흰점박이꽃무지를 식품원료화 하기 위한 전처리 조건을 설정하기 위해 세포독성, 이취감소 및 살균조건을 확립하였다. 유충에 살균 후 공급되는 세 가지 사료(참나무톱밥, 볏짚, 목초) 중에서 참나무톱밥을 공급하여 사육한 유충의 분말이 세포독성이 없었고, 유충의 발육단계를 크게 3령 유충, 용화직전 번데기, 번데기로 구분지은 후 각각의 분말이 가지는 세포독성 유무를 확인한 결과에서 세포독성은 나타나지 않았다. 세포독성 검사 결과를 토대로 식품원료화하기 위한 유충의 표준사료는 세포독성이 전혀 나타나지 않고 전국 사육농가 중 가장 많이 이용하고 있는 발효 참나무 톱밥을 표준사료로 선정하였다. 흰점박이꽃무지의 분말 전처리 조건으로 증기로 5분 동안 살균처리를 통해 사료 내의 미생물을 제거한 참나무톱밥을 급여하고 3일간 절식상태로 배변유도 후, 중장에 존재하는 내용물의 잔여도가 현저히 감소됨을 확인하였다. 또한 관능평가를 통해 3일간의 배변된 유충의 장 내용물의 잔여물이 감소함으로써 유충이 가지는 고유한 이취와 색깔이 눈에 띄게 개선되는 효과를 확인할 수 있었다. 배변 유도된 유충을 $115^{\circ}C$, $0.9kgf/cm^3$의 고온고압멸균을 5분간 실시한 후 동결건조 한 분말에서 세균 및 진균을 포함하는 미생물이 완전히 제거됨으로서 식품원료로서의 안전성을 확보할 수 있었다.

Keywords

References

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