Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Optimization in Detecting Multiply-charged Protein Ions using MALDI TOF-MS

  • Lee, Jihyeon (Department of Chemistry, Chungnam National University) ;
  • Hong, Jangmi (Department of Chemistry, Chungnam National University) ;
  • Kim, Taehee (Department of Chemistry, Chungnam National University) ;
  • Kim, Jeongkwon (Department of Chemistry, Chungnam National University)
  • Received : 2013.03.11
  • Accepted : 2013.03.20
  • Published : 2013.03.29
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Abstract

The effects of trifluoroacetic acid (TFA) were evaluated on the generation of multiply charged ions of cytochrome c in a 2-nitrophloroglucinol (2-NPG) matrix in high-vacuum, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The presence of 1% TFA in the 2-NPG matrix solution was more effective in generating multiply charged protein ions than matrix solutions containing 0.1% or 0% TFA. Regarding the matrix itself, with 1% TFA, 2-NPG was significantly more effective in generating multiply charged ions than 2,5-dihydroxybenzoic acid (2,5-DHB). The maximum charge state of cytochrome c was +8 when using a 2-NPG matrix containing 1% TFA.

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References

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