Journal of the Korean Society of Physical Medicine (대한물리의학회지)

The Korean Society of Physical Medicine (대한물리의학회)
권호 표지
DOI QR코드

DOI QR Code

Pulsed Electromagnetic Field and Pulsed Ultrasound Increases Chondrogenesis through HSP70 Overexpression in Rat Articular Cartilage

흰쥐의 관절연골에서 맥동전자장과 맥동초음파가 HSP70의 과발현을 통한 연골형성에 미치는 영향

  • Koo, Hyun-Mo (Department of Physical Therapy, College of Science, Kyungsung University)
  • 구현모 (경성대학교 이과대학 물리치료학과)
  • Received : 2013.01.17
  • Accepted : 2013.02.13
  • Published : 2013.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

연구목적: 본 연구는 흰쥐를 대상으로 정강뼈의 관절연골에 적용한 맥동전자장과 맥동초음파가 HSP70(Heat shock protein 70)의 발현을 통한 연골 형성에 미치는 영향을 알아보고자 실시하였다. 연구방법: 36마리의 200~250g의 Sprague-Dawley 흰쥐를 대조군, 맥동전자장 적용군, 맥동초음파 적용군으로 각 집단별로 12마리씩 무작위 배정하여 실험을 진행하였다. 맥동전자장은 27.12 MHz의 주파수, 5가우스의 강도, 450 W의 출력으로 10분간 적용하였고, 맥동초음파는 20%의 맥동비, 1MHz의 주파수, $1.5W/cm^2$의 강도로 10분간 적용하였다. 연구결과: 맥동전자장 적용군과 맥동초음파 적용군의 관절연골 조직에서 유의한 수준의 HSP70 발현량을 나타냈다. 또한 맥동전자장 적용군과 맥동초음파 적용군에서는 Akt, Erk1, CREB의 높은 활성도를 나타내었고, 맥동초음파 적용군에 비해서 맥동전자장 적용군의 더 높은 수준의 활성도를 보였다. 결론: 맥동전자장과 맥동초음파는 HSP70의 과발현을 유발하고, 이를 통해 연골형성을 증가시키는 것으로 나타나, 향후 관절연골의 손상에 대한 임상적 적용을 위한 추가적인 연구가 진행되어야 할 것으로 생각된다.

Keywords

References

  1. Archer CW, Francis-West P. The chondrocyte. Int J Biochem Cell Biol. 2003;35(4):401-4. https://doi.org/10.1016/S1357-2725(02)00301-1
  2. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-54. https://doi.org/10.1016/0003-2697(76)90527-3
  3. Calderwood SK, Mambula SS, Gray PJ Jr et al. Extracellular heat shock proteins in cell signaling. FEBS Lett. 2007;581(19):3689-94. https://doi.org/10.1016/j.febslet.2007.04.044
  4. Chan YS, Hsu KY, Kuo CH et al. Using low-intensity pulsed ultrasound to improve muscle healing after laceration injury: an in vitro and in vivo study. Ultrasound Med Biol. 2010;36(5):743-51. https://doi.org/10.1016/j.ultrasmedbio.2010.02.010
  5. Ciombor D, Aaron RK, S Wang et al. Modification of osteoarthritis by pulsed electromagnetic field-a morphological study. Osteoarthritis Cartilage. 2003; 11(6):455-62. https://doi.org/10.1016/S1063-4584(03)00083-9
  6. Huang MH, Tsau JC, Ding HJ et al. The role of mucopolysaccharide induction in treatment of experimental osteoarthritis in rats by ultrasound. Kaohsiung J Med Sci. 1997;13(11):661-70.
  7. Hunziker EB. Articular cartilagerepair: basic science and clinical progress. a review of the current status and prospects. Osteoarthritis Cartilage. 2002;10(6):432-63. https://doi.org/10.1053/joca.2002.0801
  8. Jacobson JI, Gorman R, Yamanashi WS et al. Low-amplitude, extremely low frequency magnetic fields for the treatment of osteoarthritic knees: a double-blind clinical study. Altern Ther Health Med. 2001;7(5): 54-64.
  9. Kiang JG, Tsokos GC. Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology. Pharmacol Ther. 1998;80(2):183-201. https://doi.org/10.1016/S0163-7258(98)00028-X
  10. Kita K, Kimura T, Nakamura N et al. PI3K/Akt signalling as a key regulatory pathway for chondrocyte terminal differentiation. Genes Cells. 2008;13(8):839-50. https://doi.org/10.1111/j.1365-2443.2008.01209.x
  11. Korstjens CM, van der Rijt RH, Albert GH et al. Low-intensity pulsed ultrasound affects human articular chondrocytes in vitro. Med Biol Eng Comput. 2008;46(12):1263-70. https://doi.org/10.1007/s11517-008-0409-9
  12. Kubo T, Arai Y, Takahashi K et al. Expression of transduced HSP70 gene protects chondrocytes from stress. J Rheumatol. 2001;28(2):330-5.
  13. Lancaster GI, Febbraio MA. Exosome-dependent trafficking of HSP70: a novel secretory pathway for cellular stress proteins. J Biol Chem. 2005;280(24):23349-55. https://doi.org/10.1074/jbc.M502017200
  14. Li H, Zhang YY, Tan HW et al. Therapeutic effect of tripterine on adjuvant arthritis in rats. J Ethnopharmacol. 2008;118(3):479-84. https://doi.org/10.1016/j.jep.2008.05.028
  15. Li J, Zhao Z, Yang J et al. p38 MAPK mediated in compressive stress-induced chondrogenesis of rat bone marrow MSCs in 3D alginate scaffolds. J Cell Physiol. 2009;221(3):609-17. https://doi.org/10.1002/jcp.21890
  16. Liu H, Lees P, Abbott J et al. Pulsed electromagnetic fields preserve proteoglycan composition of extracellular matrix in embryonic chick sternal cartilage. Biochim Biophys Acta, 1997;1336(2):303-14. https://doi.org/10.1016/S0304-4165(97)00044-5
  17. Nicolakis P, Kollmitzer J, Crevenna R et al. Pulsed magnetic field therapy for osteoarthritis of knee-a double-blind sham-controlled trial. Wien Klin Wochenschr. 2002;114(15-16):678-84.
  18. National Institutes of Health. Guide for the care and use of laboratory animals. 8th ed. Washington D.C. The National Academies Press. 1996.
  19. Nguyen JV, Marks R. Pulsed electromagnetic fields for treating osteoarthritis. Physiotherapy. 2002;88(3):458-70. https://doi.org/10.1016/S0031-9406(05)60848-6
  20. Park HG, Han SI, Oh SY et al. Cellular responses to mild heat stress. Cell Mol Life Sci. 2005;62(1):10-23. https://doi.org/10.1007/s00018-004-4208-7
  21. Sedlackova L, Nguyen TT, Zlack D et al. Cell surface and relative mRNA expression of heat shock protein 70 in human synovial cells. Autoimmunity. 2009; 42(1)17-24.
  22. ter Haar G. Therapeutic applications of ultrasound. Prog Biophys Mol Biol. 2007;93(1-3):111-29. https://doi.org/10.1016/j.pbiomolbio.2006.07.005
  23. Thamsborg G, Florescu A, Oturai P et al. Treatment of knee osteoarthritis with pulsed electromagnetic fields: a randomized, double-blind, placebo-controlled study. Osteoarthritis and Cartilage. 2005;13(7):575-81. https://doi.org/10.1016/j.joca.2005.02.012
  24. Wang Z, Jin H, Li C et al. Heat shock protein 72 protects kidney proximal tubule cells from injury induced by triptolide by means of activation of the MEK/ERK pathway. Int J Toxicol. 2009;28(3):177-89. https://doi.org/10.1177/1091581809337418