Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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The Effects of Dietary Supplementation of Vitamin C and E on the Growth Performance and the Stress Response in Broiler Chickens

육계에서 비타민 C 및 E의 첨가 급여가 성장 능력과 스트레스 반응에 미치는 영향

  • Sohn, Sea Hwan (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Cho, Eun Jung (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Jang, In Surk (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Moon, Yang Soo (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology)
  • 손시환 (경남과학기술대학교 동물생명과학과) ;
  • 조은정 (경남과학기술대학교 동물생명과학과) ;
  • 장인석 (경남과학기술대학교 동물생명과학과) ;
  • 문양수 (경남과학기술대학교 동물생명과학과)
  • Received : 2013.02.12
  • Accepted : 2013.03.15
  • Published : 2013.03.31
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Abstract

This study was performed to investigate the investigated effects of dietary supplementation of vitamin C and E on the growth performance and stress response in broiler chickens. Stress response was analyzed by the quantity of telomeric DNA, the rate of DNA damage and the expression levels of heat shock proteins (HSPs) and hydroxyl-3-methyl-glutaryl coenzyme A reductase (HMGCR) genes on tissues and blood. The telomere length and telomere shortening rates were analyzed by quantitative fluorescence in situ hybridization on the nuclei of lymphocytes and tissues. The DNA damage rate of lymphocytes was quantified by the comet assay. The expression levels of HSP70, HSP90s and HMGCR genes were measured by quantitative real-time polymerase chain reaction in lymphocytes. In results, there was no significant difference among treatments in body weight, weight gain, feed intake and mortality. The telomere shortening rate of the lymphocytes was significantly lower in the vitamin E supplemented group than the control group. The DNA damage was also decreased supplemented with vitamin C and E, as compared to the control group. The vitamin E supplemented group had a significant positive effect on the expressions of HMGCR, HSP90-${\alpha}$ and HSP90-${\beta}$ in lymphocytes, but had no significance on HSP70, as compared to the control group. We concluded that the dietary supplementation of vitamin E (100 mg/kg feed) had reduced the individual physiological stress response without stunt growth in broiler chickens.

본 연구는 브로일러에서 비타민 C와 E의 첨가 급여가 성장 능력 및 개체별 스트레스 경감 정도에 미치는 영향을 살펴보고자 하였다. 스트레스 반응 정도는 혈액과 각 조직별 세포들에 대한 텔로미어 함량, DNA 손상율 및 열손상단백질 유전자(HSP, HMGCR) 발현율을 분석하고 고찰하였다. 텔로미어 함량 및 감축율은 양적 형광접합보인법(Q-FISH)으로 분석하였고, DNA 손상율은 comet assay로 분석하였다. 열손상단백질 유전자 발현율은 HSP70, HSP90-${\alpha}$, HSP90-${\beta}$ 및 HMGCR을 표적으로 하여 real-time PCR로 분석하였다. 시험 결과, 급여 처리구 간에 체중, 증체량, 사료 섭취량, 사료 요구율 및 생존율 등 생산 능력의 차이는 없는 것으로 나타났다. 텔로미어 감축율에 있어서는 비타민 E 첨가 급여구가 대조구에 비해 유의하게 낮은 감축율을 보여 스트레스 경감의 효과를 나타내었다. DNA 손상율 또한 모든 비타민 첨가 급여구가 대조구에 비해 유의하게 낮은 양상을 보였다. HMGCR, HSP90-${\alpha}$ 및 HSP90-${\beta}$의 유전자 발현율에 있어서도 비타민 E 첨가 급여구가 대조구에 비해 유의하게 낮은 발현율을 나타내어 스트레스 경감 효과를 나타내었다. 이상의 결과에 따라 브로일러에 사료 내 비타민 E의 첨가 급여(100 mg/kg feed)는 성장 능력의 저하 없이 개체의 생리적 스트레스 정도를 경감시키는 바람직한 항산화 제재로 사료된다.

Keywords

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