Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Genotypes and Infection Status of Human Enterovirus Associated with Enteric and Respiratory Patients in Seoul, Korea

서울시내 소화기 및 호흡기 환자에서 분리한 엔테로바이러스 감염실태 및 유전형분석

  • Jang, Jungim (Seoul metropolitan Government Institute of Health & Environment) ;
  • Oh, Seah (Seoul metropolitan Government Institute of Health & Environment) ;
  • Park, SangHun (Seoul metropolitan Government Institute of Health & Environment) ;
  • Ham, HeeJin (Seoul metropolitan Government Institute of Health & Environment) ;
  • Jo, Sukju (Seoul metropolitan Government Institute of Health & Environment) ;
  • Choi, Sungmin (Seoul metropolitan Government Institute of Health & Environment) ;
  • Kang, Byunghak (Center for Infectious Diseases, Korea National Institute of Health) ;
  • Hwang, SeoYeon (Center for Infectious Diseases, Korea National Institute of Health) ;
  • Kim, JinSeok (Center for Infectious Diseases, Korea National Institute of Health)
  • 장정임 (서울특별시 보건환경연구원) ;
  • 오세아 (서울특별시 보건환경연구원) ;
  • 박상훈 (서울특별시 보건환경연구원) ;
  • 함희진 (서울특별시 보건환경연구원) ;
  • 조석주 (서울특별시 보건환경연구원) ;
  • 최성민 (서울특별시 보건환경연구원) ;
  • 강병학 (질병관리본부 국립보건연구원 감염병센터) ;
  • 황서연 (질병관리본부 국립보건연구원 감염병센터) ;
  • 김진석 (질병관리본부 국립보건연구원 감염병센터)
  • Received : 2012.03.13
  • Accepted : 2013.04.24
  • Published : 2013.04.30
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Abstract

Objective: Human enteroviruses (HEVs) are a common causative agent of gastrointestinal or respiratory infections. In this study, to examine the genotypic diversity and characteristics of HEVs associated with patients in Seoul, we collected and analyzed stool and throat swab samples taken from patients with acute gastroenteritis or a common cold from 2011 to 2012. We researched the difference in genetic characteristics of HEVs from gastroenteritis and respiratory patients. Methods: For genetic analysis, we amplified the 5'-noncoding region and partial VP1 region of HEVs by RT-PCR. The genotypes of HEVs were further identified based on nucleotide sequences of the VP1 region. Results: The majority of the HEV infections in Seoul occurred from June to August. The molecular characteristic assay showed that although the majority of HEVs can be propagated by a fecal-oral route, Coxsackievirus A2 (n=13, 19.4%), A4 (n=8, 11.9%), and A5 (n=4, 6.0%) can be preferentially transmitted by a respiratory route. Conclusions: This Enterovirus surveillance system plays an important role in preparing for a severe outbreak. The genotypic characteristics of HEV may provide potentially useful data needed for epidemiological studies.

Keywords

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