Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Identification of 3 Low-molecular Compounds from Pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) Fruit Peel

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 3종의 저분자 화합물의 단리·동정

  • Lee, Yu Geon (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Cho, Jeong-Yong (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Kim, Chan-Mi (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Jeong, Hang-Yeon (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Lee, DongI (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Kim, Soo Ro (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Lee, Sang-Hyen (Korea Pear Research Organization Chonnam National University) ;
  • Kim, Wol-Soo (Korea Pear Research Organization Chonnam National University) ;
  • Park, Keun-Hyung (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University) ;
  • Moon, Jae-Hak (Department of Food Science and Technology, and Functional Food Research Center, Chonnam National University)
  • 이유건 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 조정용 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 김찬미 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 정항연 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 이동이 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 김수로 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 이상현 (전남대학교 배 수출사업단) ;
  • 김월수 (전남대학교 배 수출사업단) ;
  • 박근형 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 문제학 (전남대학교 식품공학과 및 기능성식품연구센터)
  • Received : 2013.02.15
  • Accepted : 2013.03.12
  • Published : 2013.04.30
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Abstract

Three low-molecular compounds were isolated from methanol extracts of pear (Pyrus pyrifolia N. cv. Chuhwangbae) fruit peels using solvent fractionation, various types of column chromatogrphy (Diaion HP-20, Sephadex LH-20, and silica gel), and high performance liquid chromatography with an assay guided by 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity. The isolated compounds were identified as 2-carboxyl-4(1H)-quinolinone (kynurenic acid, 1) from butanol fraction, cis-p-coumaric acid (2) from ethyl acetate-acidic fraction, and vanillin (3) from the ethyl acetate-phenolic fraction, respectively. These isolated compounds were confirmed on the basis of the spectroscopic data of electrospray ionization mass spectrometry and nuclear magnetic resonance. This is the first time that compounds 1-3 were isolated and identified in pear.

본 연구에서는 동양배에 함유된 유용성분을 분자수준에서 밝히고자, 우리나라에서 가장 많이 섭취되고 있는 품종 중의 하나인 추황배를 대상으로 배 과피의 MeOH 추출물을 해리성을 이용하여 용매분획하였다. 얻어진 획분들을 대상으로 항산화 활성에 초점을 맞추어 open column chromatography와 high performance liquid chromatography를 행하여 3종의 항산화 화합물을 단리하였다, 그 결과, BuOH 획분으로부터 화합물 1을, EtOAc-산성 획분으로부터 화합물 2를, 그리고 EtOAc-페놀성 획분으로부터 화합물 3을 단리하고, 각각 NMR 및 MS 분석을 행하여 구조해석을 행하였다. 그 결과, 화합물 1-3은 각각 2-carboxyl 4(1H)-quinolinone (kynurenic acid, 화합물 1), cis-coumaric acid (화합물 2), 그리고 vanillin(화합물 3)으로 동정되었다. 이 3종의 화합물 모두 본 연구에 의해 배로부터 처음 동정되었다. 본 연구결과가 배의 유용성 및 기능성 연구를 위한 기초자료로 활용되길 기대한다.

Keywords

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