Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Analysis and Risk Assessment of Aflatoxin M1 in Infant Formula

분유 중 아플라톡신 M1 분석 및 위해평가

  • Kang, YoungWoon (Food Contaminants Division, Food Safety Evaluation Department, Ministry of Food and Safety) ;
  • Song, Jeong-Eon (Food Contaminants Division, Food Safety Evaluation Department, Ministry of Food and Safety) ;
  • Suh, Junghyuck (Food Contaminants Division, Food Safety Evaluation Department, Ministry of Food and Safety) ;
  • Park, Sung Kug (Food Contaminants Division, Food Safety Evaluation Department, Ministry of Food and Safety) ;
  • Kim, Meehye (Food Contaminants Division, Food Safety Evaluation Department, Ministry of Food and Safety)
  • 강영운 (식품의약품안전처 식품위해평가부 오염물질과) ;
  • 송정언 (식품의약품안전처 식품위해평가부 오염물질과) ;
  • 서정혁 (식품의약품안전처 식품위해평가부 오염물질과) ;
  • 박성국 (식품의약품안전처 식품위해평가부 오염물질과) ;
  • 김미혜 (식품의약품안전처 식품위해평가부 오염물질과)
  • Received : 2012.05.31
  • Accepted : 2013.03.07
  • Published : 2013.04.30
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Abstract

To analyze aflatoxin $M_1$ ($AFM_1$), we dissolved infant formula in warm water and cleaned it by using an immunoaffinity column (IAC). The amount of $AFM_1$ was determined by high-performance liquid chromatography coupled with fluorescence detection. $AFM_1$ was detected in 281 of 439 samples. Thus, the detection rate of $AFM_1$ was 64.0%. The average concentration of $AFM_1$ in positive samples was 2.6 ng/kg (of prepared formula). The estimated daily intake (EDI) of $AFM_1$ through infant formula was 0.087-0.646 ng/kg body weight/day and the additional number of cases of liver cancer associated with exposure to $AFM_1$ would be 0.003-0.020 cancer cases/1,000,000. Because there is less than 1 cancer case/1,000,000 per year, the exposure to $AFM_1$ through infant formula in Korea is considered to be an unlikely human health concern.

본 연구에서는 분유 중 아플라톡신 $M_1$을 면역친화성칼럼을 이용하여 정제하고 HPLC로 분리하였으며 형광검출기로 정량 분석하는 시험법을 확립하였다. 이 시험법을 이용하여 439건의 시료에 대한 분석을 실시하였다. 그 결과, 아플라톡신 $M_1$의 검출농도는 평균 2.6 ng/kg(불검출-14.9 ng/kg)로 나타났으며, 검출률은 64%로 조사되었다. 본 연구의 오염도자료를 이용한 분유 중 아플라톡신 $M_1$의 노출량 및 위해 수준은 연령별로 비교했을 때 신생아에게서 가장 높게 나타났으나 초과간암발생률은 1년에 백만명 중 0.02명 정도로서 위해수준은 매우 낮은 것으로 평가 되었다.

Keywords

References

  1. JECFA. Safety evaluation of certain mycotoxins in food. WHO Technical Report Series No 906. Joint FAO/WHO Expert Committee on Food Additive, Geneva, Switzerland. pp. 8-16 (2002)
  2. Sargeant KCRBAAR. Chemistry and origin of aflatoxins. Chemical Ind. London, UK. pp. 53-55 (1963)
  3. Wang JS, Groopman JD. DNA damage by mycotoxins. Mutat Res. 424: 167-181 (1999) https://doi.org/10.1016/S0027-5107(99)00017-2
  4. Buchi G, Spitzner D, Paglialunga S, Wogan GN. Synthesis and toxicity evaluation of aflatoxin P1. Life Sci. 13: 1143-1149 (1973) https://doi.org/10.1016/0024-3205(73)90381-0
  5. Purchase IFH. Acute toxicity of aflatoxin $M_{1}$ and $M_{2}$ in one-dayold ducklings. Food Cosmet. Toxicol. 5: 339-342 (1967) https://doi.org/10.1016/S0015-6264(67)83060-8
  6. Henry SH, Whitaker T, Rabbani I, Bowers J, Park D, Price W, Bosch FX, Pennington J, Verger P, Yoshizawa T, van Egmond H, Jonker MA, Coker R. Evaluation of certain mycotoxins. WHO Food Additives Series, No. 47/FAO Food and Nutrition Paper 74 (2001)
  7. Lee MS, Kim DH, Kim H, Lee HS, Kim CY, Park TS, Yoo KY, Park BJ, Ahn YO. Hepatitis B vaccination and reduced risk of primary liver cancer among male adults: A cohort study in Korea. Int. J. Epidemiol. 27: 316-319 (1998) https://doi.org/10.1093/ije/27.2.316
  8. Yo Ahn. strategy for vaccination against hepatitis B in areas with high endemicity: focus in Korea. Gut. 38: S63-S66 (1996) https://doi.org/10.1136/gut.38.Suppl_2.S63
  9. Lin LC, Liu FM, Fu YM, Shih DYC. Survey of aflatoxin M1 contamination of dairy products in Taiwan. J. Food Drug Anal. 12(2): 154-160 (2004)
  10. Shundo L, Navas SA, Lamardo LCA, Ruvieri V, Sabino M. Estimate of aflatoxin M1 exposure in milk and occurrence in Brazil. Food Control. 20: 655-657 (2009) https://doi.org/10.1016/j.foodcont.2008.09.019
  11. Oliveira CAF, Ferraz JCO. Occurrence of aflatoxin M1 in pasteurised, UHT milk and milk powder from goat origin. Food Control. 18: 375-378 (2007) https://doi.org/10.1016/j.foodcont.2005.11.003
  12. Chen CY, Li WJ, Peng KY. Determination of aflatoxin M1 in milk and milk powder using high-flow solid-phase extraction and liquid chromatography-tandem mass spectrometry. J. Agr. Food Chem. 53: 8474-8480 (2005) https://doi.org/10.1021/jf052142o

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