Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Protective Effects of Black Soybean Seed Coat Extracts against Oxidative Stress-induced Neurotoxicity

산화적 손상에 의해 유도된 신경세포독성에 대한 검정콩 껍질 추출물의 보호효과

  • Kwak, Ji Hyeon (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jo, Yu Na (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Ji Hee (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Hyeon Ju (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Su Il (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho Jin (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 곽지현 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 조유나 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 정지희 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 김현주 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 진수일 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 최성길 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • Received : 2013.01.31
  • Accepted : 2013.02.24
  • Published : 2013.04.30
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Abstract

Rat pheochromocytoma cells (PC12) and mice were utilized as in vitro and in vivo models to determine the neuroprotective effects of a 70% acetone extract of black soybean seed coat (BSSCE). BSSCE showed higher total phenolic contents than other extracts. Intracellular reactive oxygen species accumulation from $H_2O_2$ treatment of PC12 cells was significantly reduced when BSSCE was present in the media compared to PC12 cells treated with $H_2O_2$ only. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide (MTT) reduction assay and lactate dehydrogenase assay also showed significantly increased protective effects in PC12 cells. In addition, BSSCE improved the in vivo cognitive ability against amyloid beta peptide-induced neuronal deficits.

본 연구는 산화적 스트레스로부터 유도되는 신경세포 사멸을 보호할 수 있는 생리활성물질을 탐색하기 위하여 검정콩 껍질 추출물의 항산화 효과 및 PC12 신경세포 보호효과에 대하여 조사하였다. 다양한 용매에 의해 추출된 검정콩 껍질 추출물의 총 페놀화합물 함량을 측정한 결과 70% acetone 추출물이 다른 용매추출물에 비하여 가장 높은 총 페놀화합물 함량을 나타내었으며, 이를 기초로 70% acetone 추출물을 이용한 세포 내 항산화 활성도 높은 것으로 나타났다. 또한 PC12 신경세포 보호효과를 조사한 결과, 세포생존율과 세포막 손상 보호효과에서 70% acetone 추출물 처리구는 최대 $500{\mu}g/mL$까지 $H_2O_2$를 처리한 대조구에 비하여 농도 의존적으로 신경세포 보호효과가 나타남을 알 수 있었다. 동일 추출물을 활용한 in vivo 인지학습능력 평가에서도 대조구인 $A{\beta}$ ICV injection을 한 group과 비교하였을 때 전체적으로 추출물의 농도가 증가할수록 Y-maze test와 passive avoidance test에서 control구와 유사하거나 높은 인지 및 기억 능력 회복효과를 보여주었다. 본 연구결과를 종합해 보면 BSSCE는 항산화, 뇌 신경세포 보호효과 및 in vivo 인지 기억 회복능을 갖는 것으로 판단되며, 추후 검정콩 껍질에 존재하는 유효 생리활성물질을 확인함으로써 현대 사회의 가장 큰 질병 중 하나인 알츠하이머성 치매(AD)와 같은 퇴행성 뇌신경질환 예방에 유용한 소재가 될 것으로 판단된다.

Keywords

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