Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of Housing Systems on Physiological and Immunological Parameters in Laying Hens

  • Kang, Sung-Young (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Ko, Young-Hyun (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Moon, Yang-Soo (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Sohn, Sea-Hwan (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Jang, In-Surk (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
  • Received : 2013.02.07
  • Accepted : 2013.04.24
  • Published : 2013.04.30
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Abstract

The aim of this study was to assess the effects of housing systems on physiological and immunological responses as stress indicators in laying hens. A total of 500 White Leghorn aged 16 weeks were allotted into ten conventional cages (10 birds/cage and 810 $cm^2$/bird) and four floor pens (100 birds/pen and 2,800 $cm^2$/bird) for 24 weeks. The hens housed in conventional cages with higher stocking density resulted in a significantly (P<0.05) lower BW compared with those housed in floor pens with lower stocking density without affecting the relative weights of immune organs between housing conditions. In plasma biochemical values, cholesterol and corticosterone were significantly (P<0.05) lower in the hens housed in floor pens compared with those housed in conventional cages. In pro-inflammatory cytokines, hepatic interleukin (IL)-10 and interferon-gamma (IFN-${\gamma}$) levels were significantly (P<0.05) higher in the hens housed in conventional cages compared with those kept in floor pens. Splenic and thymic IFN-${\gamma}$ expression was significantly (P<0.05) up-regulated in the hens kept in conventional cages compared with those kept in floor pens without affecting IL-1, IL-10, lipopolysaccharide- induced tumor necrosis factor-${\alpha}$ factor (LITAF) and inducible nitric oxide synthase (iNOS). In the bursa of Fabricius, IL-10 and iNOS expression of the hens housed in conventional cages were significantly (P<0.05) higher compared with those of the hens housed in floor pens. In conclusion, layers housed in conventional cages enhanced plasma cholesterol, corticosterone and some pro-inflammatory cytokines in the immune organs compared with those in floor pens.

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References

  1. Albentosa, M. J. and Cooper, J. J. 2004. Effects of cage light and stocking on the frequency of comfort behaviors performed by laying hens in furnished cages. Anim. Welfare 13:419-424.
  2. Awadalla, S. F. 1998. Effect of some stressors on pathogenicity of Eimeria tenella in broiler chicken. J. Egypt. Soc. Parasitol. 28: 683-690.
  3. Baxter, M. R. 1994. The welfare problems of laying hens in battery cages. Vet. Rec. 134:614-619. https://doi.org/10.1136/vr.134.24.614
  4. Buijs, S., Keeling, L., Rettenbacher, S., Van Poucke, E. and Tuyttens, F. A. 2009. Stocking density effects on broiler welfare: identifying sensitive ranges for different indicators. Poult. Sci. 88(8):1536-1543. https://doi.org/10.3382/ps.2009-00007
  5. Cheng, H. W. and Muri, W. M. 2004. Chronic social stress differentially regulates neuroendocrine response in laying hens: II. Genetic basis of adrenal responses under three different social conditions. Psychoneurodenocrinlogy 29:961-971. https://doi.org/10.1016/j.psyneuen.2003.09.002
  6. Cook, M. E. 1991. Nutrition and immune response of the domestic fowl. CRC Crit. Rev. Poult. Biol. 3:167-189.
  7. Fahey, A. G. and Cheng, H. W. 2008. Effects of social disruption on physical parameter corticosterone concentrations, and immune system in two genetic lines of White Leghorn layers. Poult. Sci. 87(10):1947-1954. https://doi.org/10.3382/ps.2007-00477
  8. Felten, S. Y., Madden, K. S., Bellinger, D. L., Kruszewska, B., Moynihan, J. A. and Felten, D. L. 1998. The role of the sympathetic nervous system in the modulation of immune responses. Adv. Pharmacol. 42:583-587.
  9. Freire, R., Wilkins, L. J., Short, F. and Nicol, C. J. 2003. Behaviour and welfare of individual laying hens in a non-cage system. Br. Poult. Sci. 44:22-29. https://doi.org/10.1080/0007166031000085391
  10. Gibson, S. W., Hughes, B. D., Harvey, S. and Dun, P. 1986. Plasma concentrations of corticosterone and thyroid hormones in laying fowls from different housing system. Br. Poult. Sci. 27(4):621-628. https://doi.org/10.1080/00071668608416921
  11. Groux, H. and Powrie, F. 1999. Regulatory T cells and inflammatory bowel disease. Immunol. Today. 20:442-445. https://doi.org/10.1016/S0167-5699(99)01510-8
  12. Guzik, T. J., Korbut, R. and Adamek-Guzik, T. 2003. Nitric oxide and superoxide in inflammation and immune regulation. J. Physiol. Pharmacol. 54(4):469-487.
  13. Haller, J., Millar, S., van de Schraaf, J., de Kloet, R. E. and Kruk, M. R. 2000. The active phase-related increase in corticosterone and aggression are linked. J. Neuroendocrinol. 12(5):431-436.
  14. Hangalapura, B. N., Kaiser, M. G., Poel, J. J., Parmentier, H. K. and Lamont, S. J. 2006. Cold stress equally enhances in vivo pro-inflammatory cytokine gene expression in chicken lines divergently selected for antibody responses. Dev. Comp. Immunol. 30(5):503-511. https://doi.org/10.1016/j.dci.2005.07.001
  15. Hong, Y. H., Lillehoj, H. S., Lee, S. H., Park, D. W. and Lillehoj, E. P. 2006. Molecular cloning and characterization of chicken lipopolysaccharide-induced TNF-alpha factor (LITAF). Dev. Comp. Immunol. 30(10):919-929. https://doi.org/10.1016/j.dci.2005.12.007
  16. Janeway, C. A., Travers, P., Walport, M. and Shlomchik, M. 2001. The adaptive immune responses. Pages 295-381 in Immunology V. ed. C. A. Janeway. Garland Publishing New York, NY.
  17. Kaiser, P., Wu, Z., Rothwell, L., Fife, M., Gibson, M., Poh, T. Y., Shini, A., Bryden, A. W. and Shini, A. 2009. Prospects for understanding immune-endocrine interactions in the chicken. Gen. Comp. Endocrinol. 163(1-2):83-91. https://doi.org/10.1016/j.ygcen.2008.09.013
  18. Kang, S. Y., Ko, T. H., Moon, Y. S., Sohn, S. H. and Jang, I. S. 2011. Effect of combined stress induced by stocking density and feed restriction on hematological and cytokine parameters as stress indicators in laying hens. Asian-Aust. J. Anim. Sci. 24(3):414-420. https://doi.org/10.5713/ajas.2011.10315
  19. Mashaly, M. M., Hendricks 3rd, G. L., Kalama, M. A., Gehad, A. E., Abbas, A. O. and Patterson, P. H. 2004. Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult. Sci. 83(6):889-894. https://doi.org/10.1093/ps/83.6.889
  20. Mashaly, M. M., Webb, M. L., Youtz, S. L., Roush, W. B. and Graves, H. B. 1984. Changes in serum corticosterone concentration of laying hens as a response to increased population density. Poult.Sci. 63(11):2271-2274. https://doi.org/10.3382/ps.0632271
  21. Nicol, C. J., Brown, S. N., Glen, E., Pope, S. J., Short, F. J., Warriss, P. D., Zimmerman, P. H. and Wilkins, L. J. 2006. Effects of stocking density, flock size and management on the welfare of laying hens in single-tier aviaries. Br. Poult. Sci. 47(2):135-146. https://doi.org/10.1080/00071660600610609
  22. Nicol, C. J. 1987. Behavioural responses of laying hens following aperiod of spatial restriction. Anim. Behav. 35:522-527.
  23. Platz, S., Heyn, E., Hergt, F., Weigl, B. and Erhard, M. 2009. Comparative study on the behaviour, health and productivity of laying hens in a furnished cage and an aviary system. Berl. Munch. Tierarztl. Wochenschr. 122(7-8):235-240.
  24. Pohle, K. and Cheng, H. W. 2009. Comparative effects of furnished and battery cages on egg production and physiological parameters in White Leghorn hens. Poult. Sci. 88:2042-2051. https://doi.org/10.3382/ps.2009-00171
  25. Puvadolpirod, S. and Thaxton, J. P. 2000. Model of physiological stress in chickens 1. Response parameters. Poult. Sci. 79(3): 363-369. https://doi.org/10.1093/ps/79.3.363
  26. Rettenbacher, S. and Palme, R. 2009. Biological validation of a non-invasive method for stress assessment in chickens. Berl. Munch. Tierarztl. Wochenschr. 122:8-12.
  27. Rodenburg, T. B., Tuyttens, F. A., Sonck, B., De Reu, K., Herman, L. and Zoons, J. 2005. Welfare, health, and hygiene of laying hens housed in furnished cages and in alternative housing systems. J. Appl. Anim. Welf. Sci. 8(3):211-26. https://doi.org/10.1207/s15327604jaws0803_5
  28. Sadeyen, J. R., Trotereau, J., Protais, J., Beaumont, C., Sellier, N., Salvat, G., Velge, P. and Lalmanach, A. C. 2006. Salmonella carrier-state in hens: study of host resistance by a gene expression approach. Microbes Infect. 8(5):1308-1314. https://doi.org/10.1016/j.micinf.2005.12.014
  29. SAS. 1989. SAS / STAT User's Guide, Version 6, 4th Edition. Vol 2. SAS Ins., Cary, NC.
  30. Sherwin, C. M., Richards, G. J. and Nicol, C. 2010. Comparison of the welfare of layer hens in 4 housing systems in the UK. Br. Poult. Sci. 51(4):488-499. https://doi.org/10.1080/00071668.2010.502518
  31. Shini, S. 2003. Physiological responses of laying hens to the alternative housing system. Int. J. Poult. Sci. 2:357-360. https://doi.org/10.3923/ijps.2003.357.360
  32. Shini, S., Huff, G. R., Shini, A. and Kaiser, P. 2010a. Understanding stress-induced immunosuppression: Exploration of cytokine and chemokine gene profiles in chicken peripheral leukocytes. Poult. Sci. 89:841-851. https://doi.org/10.3382/ps.2009-00483
  33. Shini, S., Shini, A. and Kaiser, P. 2010b. Cytokine and chemokine gene expression profiles in heterophiles from chickens treated with corticosterone. Stress. 13(3):185-194. https://doi.org/10.3109/10253890903144639
  34. Shini, S. and Kaiser, P. 2009. Effects of stress, mimicked by administration of corticosterone in drinking water, on the expression of chicken cytokine and chemokine genes in lymphocytes. Stress 12(5):388-399. https://doi.org/10.1080/10253890802526894
  35. Siegal, H. S. 1985. Immunological responses as indicators of stress. World's Poult. Sci. J. 41:36-44. https://doi.org/10.1079/WPS19850003
  36. Siegal, H. S. 1995. Stress, strains and resistance. Br. Poult. Sci. 36:3-22. https://doi.org/10.1080/00071669508417748
  37. Singh, R., Cook, N., Cheng, K. M. and Silversides, F. G. 2009. Invasive and noninvasive measurement of stress in laying hens kept in conventional cages and in floor pens. Poult. Sci. 88(7):1346-1351. https://doi.org/10.3382/ps.2008-00300
  38. Sohail, M. U., Ijaz, A., Yousaf, M. S., Ashraf, K., Zaneb, H., Aleem, M. A. and Rehman, H. 2010. Alleviation of cyclic heat stress in broilers by dietary supplementation of mannanoligosaccharide and Lactobacillus-based probiotic: Dynamics of cortisol, thyroid hormone, cholesterol, C-reactive protein, and humoral immunity. Poult. Sci. 89:1934-1938. https://doi.org/10.3382/ps.2010-00751
  39. Tempel, D. L. and Leibowitz, S. F. 1994. Adrenal steroid receptors: interactions with brain neuropeptide systems in relation to nutrient intake and metabolism. J. Neuroendocrinol. 6(5):479-501. https://doi.org/10.1111/j.1365-2826.1994.tb00611.x
  40. Zhang, Z. W., Lv, Z. H., Li, J. L., Li, S., Xu, S. W. and Wang, X. L. 2011. Effects of cold stress on nitric oxide in duodenum of chicks. Poult. Sci. 90(7):1555-1561. https://doi.org/10.3382/ps.2010-01333
  41. Zimmerman, P. H., Lindberg, A. C., Pope, S. J., Glen, E., Bolhuis, J. E. and Nicol, C. J. 2006. The effect of stocking density, flock size and modified management on laying hen behavior and welfare in a non-cage system. Appl. Anim. Behav. Sci. 101:111-124. https://doi.org/10.1016/j.applanim.2006.01.005

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