Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
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Immunohistochemical study on expression of mast cell and macrophage in irritation fibroma

자극성 섬유종에서 비만 세포와 대식 세포의 면역조직화학적 발현

  • Han, Hye-Yeon (Department of Oral Pathology, School of Dentistry, Yangsan Campus of Pusan National University) ;
  • Kang, Nam-Kyu (Department of Oral Pathology, School of Dentistry, Yangsan Campus of Pusan National University) ;
  • Ryu, Mi-Heon (Department of Oral Pathology, School of Dentistry, Yangsan Campus of Pusan National University)
  • 한혜연 (부산대학교 치의학전문대학원 구강병리학 교실) ;
  • 강남규 (부산대학교 치의학전문대학원 구강병리학 교실) ;
  • 유미현 (부산대학교 치의학전문대학원 구강병리학 교실)
  • Received : 2013.01.25
  • Accepted : 2013.02.26
  • Published : 2013.02.28
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Abstract

연구목적 : 자극성 섬유종은 만성자극에 의해 발생하는 구강내 증식성 병변이다. 상처치유의 초기 과정에서는 비만 세포와 대식 세포가 섬유모세포의 이주, 증식, 아교질합성 등에 연관되어 있는 성장인자와 사이토카인을 분비하여 상처 치유에 중요한 역할을 한다. 저자들은 자극성 섬유종을 조직학적 특성에 따라 세분하고, 각각의 조직학적 아형에서 비만 세포와 대식 세포의 발현을 조사하여 자극성 섬유종의 발생 기전을 이해하고자 하였다. 연구방법 : 본 연구에서는 82예의 자극성 섬유종을 조직 소견에 따라 4가지 유형으로 분류하였으며, 자극성 섬유종과 10예의 정상 구강점막에 톨루이딘 블루 염색과 CD 68 면역조직화학염색을 시행하였다. 이를 통계화하여 자극성 섬유종의 조직학적 아형에 따른 비만 세포와 대식 세포의 분포 정도를 관찰하였다. 연구결과 : 통계 결과 비만 세포와 대식 세포의 분포는 자극성 섬유종에서 현저히 증가하였으며, Spearman 상관계수는 0.693이었다. 결론 : 조직의 섬유화에 관여하는 비만 세포는 자극성 섬유종의 cellular type에서 유의하게 증가하였으며, 대식 세포도 자극성 섬유종의 모든 아형에서 유의하게 증가하였다. 따라서 자극성 섬유종의 형성 과정에는 비만 세포와 대식 세포의 증가가 중요한 역할을 하는 것으로 생각되었다.

Keywords

References

  1. Sapp JP, Eversole LR, Wysocki GP. Contemporary oral and maxillofacial pathology. 2nd ed. St. Louis, Mosby. 2004, 290-291.
  2. Damm DD, Bouquot JE, Neville B, Allen CM. Oral and maxillofacial pathology. 2nd ed. Philadelphia, W. B. Saunders. 2002, 438-439.
  3. Vergotine RJ. A giant cell fibroma and focal fibrous hyperplasia in a young child: a case report. Case Rep Dent 2012: 2012; 370242.
  4. Jang GW. A study of dental calculus scanning electron microscope by observation bacteria identification. J Korean Acad Dental Hygiene Education 2007; 2: 189-196.
  5. Crivellato E, Nico B, Ribatti D. The history of the controversial relationship between mast cells and basophils. Immunol Lett 2011; 141: 10-17. https://doi.org/10.1016/j.imlet.2011.06.008
  6. Farahani SS, Navabazam A, Ashkevari FS. Comparison of mast cells count in oral reactive lesions. Pathol Res Pract 2010; 206: 151-155. https://doi.org/10.1016/j.prp.2009.10.006
  7. Santos PP, Nonaka CF, Pinto LP, de Souza LB. Immunohistochemical expression of mast cell tryptase in giant cell fibroma and inflammatory fibrous hyperplasia of the oral mucosa. Arch Oral Biol 2011; 56: 231-237. https://doi.org/10.1016/j.archoralbio.2010.09.020
  8. Collington SJ, Williams TJ, Weller CL. Mechanisms underlying the localisation of mast cells in tissues. Trends Immunol 2011; 32: 478-485. https://doi.org/10.1016/j.it.2011.08.002
  9. Sabarinath B, Sriram G, Saraswathi TR, Sivapathasundharam B. Immunohistochemical evaluation of mast cells and vascular endothelial proliferation in oral submucous fibrosis. Indian J Dent Res 2011; 22: 116-121. https://doi.org/10.4103/0970-9290.80009
  10. Park JE, Barbul A. Understanding the role of immune regulation in wound healing. Am J Surg 2004; 187: 11S-16S. https://doi.org/10.1016/S0002-9610(03)00296-4
  11. Rodero MP, Khosrotehrani K. Skin wound healing modulation by macrophages. Int J Clin Exp Pathol 2010; 3: 643-653.
  12. van der Veer WM, Bloemen MC, Ulrich MM, et al. Potential cellular and molecular causes of hypertrophic scar formation. Burns 2009; 35: 15-29. https://doi.org/10.1016/j.burns.2008.06.020
  13. Lee HS, Lim TS. Comparative study on survival rate of human gingival fibroblasts stored in different storage media. J Korean Soc Dent Hyg 2012; 12: 733-739. https://doi.org/10.13065/jksdh.2012.12.4.733
  14. Shumaker PR, Kwan JM, Badiavas EV, et al. Rapid healing of scar-associated chronic wounds after ablative fractional resurfacing. Arch Dermatol 2012; 148: 1289-1293. https://doi.org/10.1001/2013.jamadermatol.256
  15. Smitha B, Donoghue M. Clinical and histopathological evaluation of collagen fiber orientation in patients with oral submucous fibrosis. J Oral Maxillofac Pathol 2011; 15: 154-160. https://doi.org/10.4103/0973-029X.84481
  16. Jackson WM, Nesti LJ, Tuan RS. Concise review: clinical translation of wound healing therapies based on mesenchymal stem cells. Stem Cells Transl Med 2012; 1: 44-50. https://doi.org/10.5966/sctm.2011-0024
  17. Shiota N, Nishikori Y, Kakizoe E, et al. Pathophysiological role of skin mast cells in wound healing after scald injury: study with mast cell-deficient W/W(V) mice. Int Arch Allergy Immunol 2009; 151: 80-88.
  18. Noli C, Miolo A. The mast cell in wound healing. Vet Dermatol 2001; 12: 303-313. https://doi.org/10.1046/j.0959-4493.2001.00272.x
  19. Gilfillan AM, Beaven MA. Regulation of mast cell responses in health and disease. Crit Rev Immunol 2011; 31: 475-529. https://doi.org/10.1615/CritRevImmunol.v31.i6.30
  20. Egozi EI, Ferreira AM, Burns AL, Gamelli RL, Dipietro LA. Mast cells modulate the inflammatory but not the proliferative response in healing wounds. Wound Repair Regen 2003; 11: 46-54. https://doi.org/10.1046/j.1524-475X.2003.11108.x
  21. Kennelly R, Conneely JB, Bouchier-Hayes D, Winter DC. Mast cells in tissue healing: from skin to the gastrointestinal tract. Curr Pharm Des 2011; 17: 3772-3775. https://doi.org/10.2174/138161211798357854
  22. Mansbridge JN, Liu K, Pinney RE, et al. Growth factors secreted by fibroblasts: role in healing diabetic foot ulcers. Diabetes Obes Metab 1999; 1: 265-279. https://doi.org/10.1046/j.1463-1326.1999.00032.x
  23. Iamaroon A, Pongsiriwet S, Jittidecharaks S, et al. Increase of mast cells and tumor angiogenesis in oral squamous cell carcinoma. J Oral Pathol Med 2003; 32: 195-199. https://doi.org/10.1034/j.1600-0714.2003.00128.x
  24. Makhlouf HR, Ishak KG. Sclerosed hemangioma and sclerosing cavernous hemangioma of the liver: a comparative clinicopathologic and immunohistochemical study with emphasis on the role of mast cells in their histogenesis. Liver 2002; 22: 70-78. https://doi.org/10.1046/j.0106-9543.2001.01604.x
  25. Wilkins BS, Buchan SL, Webster J, Jones DB. Tryptase-positive mast cells accompany lymphocytic as well as lymphoplasmacytic lymphoma infiltrates in bone marrow trephine biopsies. Histopathology 2001; 39: 150-5.
  26. Mahdavian Delavary B, van der Veer WM, van Egmond M, Niessen FB, Beelen RH. Macrophages in skin injury and repair. Immunobiology 2011; 216: 753-762. https://doi.org/10.1016/j.imbio.2011.01.001
  27. Shaker SA, Ayuob NN, Hajrah NH. Cell talk: a phenomenon observed in the keloid scar by immunohistochemical study. Appl Immunohistochem Mol Morphol 2011; 19: 153-159. https://doi.org/10.1097/PAI.0b013e3181efa2ef
  28. Shih B, Garside E, McGrouther DA, Bayat A. Molecular dissection of abnormal wound healing processes resulting in keloid disease. Wound Repair Regen 2010; 18: 139-153. https://doi.org/10.1111/j.1524-475X.2009.00553.x