International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Staphylococcus Species in the Dental and Medical Environment

  • Han, Seung-Ho (Department of Laboratory Medicine, Veterans Hospital) ;
  • Kim, Shin-Moo (Department of Clinical Laboratory Science, Wonkwang Health Science College) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Kim, Kang-Ju (Department of Oral Microbiology & Immunology, School of Dentistry, & Institute of Biotechnology Wonkwang University)
  • Received : 2012.12.07
  • Accepted : 2013.02.28
  • Published : 2013.03.31
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Abstract

Staphylococcus species are one of prevalent pathogens found in hospitals. Microbes that are a primary cause of nosocomial infection were isolated from a dental and medical environment it may assist the reader to explain what this is and how it differs from the 'dental health care providers and ward health care providers'. To investigate the distribution of staphylococcus species in this environment, we used vitek II to measure drug sensitivity, and further performed biochemical testing. The isolation rate of staphylococcus species from the dental and medical environment was 100% but from dental health care providers and ward health care providers were 44.4% and 33.3%, respectively. In the analyses, staphylococcus species showed resistance to diffusion of cefoxitin and oxacillin discs. These staphylococci may be sufficiently positive for the mecA gene. Our results suggest that staphylococci might be an important cause of nosocomial infection in the dental clinic.

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References

  1. Kundsin RB. Documentation of airborne infection during surgery. Ann N Y Acad Sci. 1980;353:255-61. https://doi.org/10.1111/j.1749-6632.1980.tb18928.x
  2. Etiene J, Fleurette J, Ninet JF, Favet P, Gruer LD. Staphylococcus endocarditis after dental extraction. Lancet. 1986;2(8505):511-2.
  3. Etiene J, Pangon B, Leport C, Wolf M, Clair B, Perronne C, Brun Y. Bure A. Staphylococcus lugdunensis endocarditis. Lancet. 1989;1: 390.
  4. Frency J, Brun Y, Bes M, Meugnier H, Grimont F, Grimon PAD, Newi C, Fleurette J. Staphylococcus lugdunensis sp. and Staphylococcus schleiferi sp. novel two species from human clinical specimens. Int Syst Bacteriol. 1998;38: 168-72.
  5. Kim SJ. Plasmid Profiles and Ribotyping of Methicillin- resistant Staphylococcus aureus. Department of Clinical Pathology, Graduate School, College of Medicine, Seoul National University. 1991.
  6. Park OH. Study on the Isolation of Bacteria from Door Knobs of Hospital. Korean Central Journal of Medicine. 1973;25:637-40.
  7. Hemming val G, Overall JC, Britt MR. Nosocomial infections in a newborn intensive-care unit. Results of forty-one months of surveillance. New Engl J Med. 1976;294(24): 1310-6. https://doi.org/10.1056/NEJM197606102942403
  8. Jones RN, Barry AL, Gardiner RV, Packer RR. The prevalence of staphylococcal resistance to penicillinaseresistant penicillins. A retrospective and prospective national surveillance trial of isolates from 40 medical centers. Diagn Microbiol Infect Dis. 1989;12(5):385-94. https://doi.org/10.1016/0732-8893(89)90108-9
  9. Maple PAC, Hamilton-Miller JMT, Brumfitt W. Worldwide antibiotic resistance in methicillin-resistant Staphylococcus aureus. Lancet. 1989;1(8637):537-540.
  10. Kugl SA, Pattee PA, Baldwin JN. Chromosomal map lo cation of the methicillin resistance determinant in Staphylococcus aureus. J Bacteriol. 1978;135(2):460-5.
  11. Ubukata K, Nonoguchi R, Matsuhashi M, Konno M. Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin resistant S. aureus- specific penicillin binding proteins. J Bacteriol. 1989; 171(5):2882-5.
  12. Inglis B, Matthews PR,Stewart PR. The expression in Staphylococcus aureus of cloned DNA encoding methicillin resistance. J Gen Microbiol. 1988;134(6):1465-9.
  13. Tesch W, Strassle A, Berger-Bachi B, O'Hara D, Reynolds P, Kayser FH. Cloning and expression of methicillin resistance from Staphylococcus epidermidis in Staphylococcus carnosus. Antimicrob Agents Chemother. 1988;32 (10):1494-9. https://doi.org/10.1128/AAC.32.10.1494
  14. Fontana R. Penicillin-binding proteins and the intrinsic resistance to $\beta$ -lactams in gram-positive cocci. J Antimicrob Chemother. 1985;16(4):412-6. https://doi.org/10.1093/jac/16.4.412
  15. Hartman BJ, Tomasz A. Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus. Antimicrob Agents Chemother. 1986;29(1):85-92. https://doi.org/10.1128/AAC.29.1.85
  16. Matsuhashi M, Song MD, Ishino F, Wachi M, Doi M, Inoue M, Ubukata K, Yamashita N, Konno M. Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to β-lactam antibiotics in Staphylococcus aureus. J Bacteriol. 1986;167(3):975-80. https://doi.org/10.1128/jb.167.3.975-980.1986
  17. Codino RC, Marshall WE. Control of infection in the dental operatory. Dent Surv. 1976:42-8.
  18. Szymanska J. Dental bioaerosol as an occupational hazard in a dentist's workplace. Ann Agric Environ Med. 2007; 14(2):203-7.
  19. Andriole VT. Pseudomonas bacteremia : Can antibiotic therapy improve survival. J Lab Clin Med. 1979;94(2): 196-200.
  20. Jarvis WR, White JW, Munn VP, Mosser JL, Emori TG, Culver DH, Thornsberry C, Hughes JM. Nosocomial infection surveillance. MMWR CDC Surveill Summ. 1984; 33(2):9SS-21SS.
  21. Boutiba-Ben Boubaker I, Ben Abbes R, Ben Abdallah H, Mamlouk K, Mamlouk K, Mahjoubi F, Kammoun A, Hammami A, Ben Redjeb S. Evaluation of a cefoxitin disk diffusion test for the routine detection of methicillinresistant Staphylococcus aureus. Clin Microbiol Infect. 2004;10(8):762-5. https://doi.org/10.1111/j.1469-0691.2004.00919.x
  22. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disk susceptibility test, 7th ed. Approved standards MA7. National Committee for Clinical Laboratory Standards. Wayne, PA, 2009.