Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species

미토콘드리아 DNA 염기서열 변이를 이용한 인삼 종 판별 연구

  • Jo, Ick Hyun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Bang, Kyong Hwan (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Chang (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Jang Uk (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Shin, Mi Ran (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Moon, Ji Young (Experiment Research Institute of National Agricultural Products Quality Management Service, MIFAFF) ;
  • Noh, Bong Soo (Department of Food Science and Technology, Seoul Women's University) ;
  • Hyun, Dong Yun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Dong Hwi (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Cha, Seon Woo (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Hong Sig (Department of Plant Resources, Chungbuk National University)
  • 조익현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김장욱 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 신미란 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 문지영 (국립농산물품질관리원 시험연구소) ;
  • 노봉수 (서울여자대학교 식품공학과) ;
  • 현동윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김홍식 (충북대학교 식물자원학과)
  • Received : 2012.01.11
  • Accepted : 2013.02.27
  • Published : 2013.04.30
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Abstract

This study describes the identification of Panax species using mitochondrial consensus primers. Initially, a total of thirty primers were tested in ten Korean ginseng cultivars and two foreign Panax species, P. quinquefolius and P. notoginseng. In the polymerase chain reaction (PCR) amplification results, three primers (cox1, nad1/2-3 and nad2/1-2) generated co-dominant polymorphic banding patterns discriminating Korean ginseng cultivars from P. quinquefolius and P. notoginseng. However, these primers could not generated polymorphisms among the Korean ginseng cultivars, and simply represented species-specific polymorphisms for P. quinquefolius and P. notoginseng. Primers PQ91 and PN418 were designed from the consensus sequence of nad1/2-3 region. Two banding patterns (A or B) were detected in PQ91. Korean ginseng cultivars and P. notoginseng shared the same banding pattern (A type) and P. quinquefolius was identified another banding pattern (B type). In the case of PN418, two banding patterns (A or B) were detected in the Korean ginseng cultivars and two foreign Panax species. Korean ginseng cultivars and P. quinquefolius shared the same banding pattern (A type) and P. notoginseng was identified another banding pattern (B type). The combination banding patterns of three Panax species, Korean ginseng cultivars (Panax ginseng C. A. Mey.), P. quinquefolius and P. notoginseng, was identified as 'AA', 'BA' and 'AB', respectively. Consequently, PQ91 and PN418 primer sets can be used to distinguish among Panax species.

Keywords

References

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