Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Repetitive Electroacupuncture Alleviate Neuropathic Pain in Association with Suppressing Activation of Spinal Glial Cells

반복적인 전침 처치의 척수 교세포 활성 억제를 통한 신경병증성 통증 억제 효과

  • Lee, Heun Joo (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Jeong, Bo Eun (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Song, Da Eun (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Park, Min Young (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Koo, Sungtae (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University)
  • 이흔주 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 정보은 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 송다은 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 박민영 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 구성태 (부산대학교 한의학전문대학원 경락구조의학부)
  • Received : 2013.03.04
  • Accepted : 2013.03.19
  • Published : 2013.03.27
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Abstract

Objectives : Effects of repetitive electroacupuncture(EA) on the pain behavior and activation of spinal glial cells were examined in the rat model of neuropathic pain. Methods : Twenty one adult male Sprague-Dawley rats were randomly assigned into 3 groups(control group, SP6 group, ST36+GB34 group). Neuropathic pain was induced by tight ligation of L5 spinal nerve. Mechanical and thermal hypersensitivity of hind paw were tested. Immunohistochemistry was performed in spinal cord L5/6 of all groups. EA was treated once in a day from the $5^{th}$ day after surgery. Results : EA treatments applied to ST36 and GB34 reduced significantly both of mechanical and thermal hypersensitivity after 3 times of treatment throughout the experiments. In the SP6 group, the analgesic effect was also shown after 7 times of treatment. Immunohistochemistry demonstrated inhibition of microglia and astrocyte activation in the spinal cord L5/6 dorsal horn in the ST36+GB34 group. Conclusions : The present results suggest that repetitive EA exert strong analgesic effect on neuropathic pain. These analgesic effects in neuropathic pain are associated with suppressing the activation of microglia and astrocyte.

목적 : 신경병증성 통증 쥐 모델에서 통증 행동과 척수 교세포의 활성화에 대한 반복적 전침의 영향을 실험하였다. 방법 : 21마리 Sprague-Dawley 성체 수컷 쥐를 무작위적으로 대조군, SP6 군, ST36+GB34 군으로 나누었다. 신경병증성 통증은 L5 척수신경을 단단하게 묶는 것으로 유도하였다. 신경병증성 통증이 유도된 뒷발에 기계적 및 열적 민감도를 조사하였다. 모든 군의 L5/6 척수에서 면역조직화학염색법을 수행하였다. 수술 이후 5일째부터 하루에 한번씩 전침을 시행하였다. 결과 : 족삼리(ST36)와 양릉천(GB34)에 적용된 전침은 3번의 처치 이후 실험기간 내내 기계적 및 열적 민감도를 모두 감소시켰다. 삼음교(SP6) 군에서는 진통 효과가 7번의 처치 이후 감소됨을 보였다. 면역조직화학염색법은 ST36+GB34 군에서 L5/6 척수 후각에서 교세포와 성상세포의 활성화가 억제됨을 보였다. 결론 : 이 결과는 반복적인 전침은 신경병증성 통증에 강한 진통 효과를 발휘 하는 것을 시사한다. 신경병증성 통증에서 이 진통 효과는 교세포와 성상세포의 활성화를 억제하는 것과 관련이 있다.

Keywords

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