Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Physicochemical and Functional Properties of Kochujang with Broccoli Leaf Powder

브로콜리잎 분말 첨가 재래식 고추장의 이화학적 및 기능적 특성

  • Oh, You-Sung (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Baek, Jin-Woo (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Park, Kyeong-Yeol (Dept. of Food Bioengineering, Jeju National University) ;
  • Hwang, Joon-Ho (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Lim, Sang-Bin (Biotechnology Regional Innovation Center, Jeju National University)
  • 오유성 (제주대학교 생명과학기술혁신센터) ;
  • 백진우 (제주대학교 생명과학기술혁신센터) ;
  • 박경열 (제주대학교 식품생명공학과) ;
  • 황준호 (제주대학교 생명과학기술혁신센터) ;
  • 임상빈 (제주대학교 생명과학기술혁신센터)
  • Received : 2013.01.21
  • Accepted : 2013.04.09
  • Published : 2013.05.31
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Abstract

Home-made broccoli Kochujang (HMBK) was prepared with the addition of 5% broccoli leaf powder. Its physicochemical and functional properties were measured in extracts (80% methanol, 80% ethanol, and distilled water) and compared with home-made Kochujang (HMK) and factory-produced Kochujang (FPK). Total phenolic content (TPC) was 22% higher in methanol extract from HMBK (524.2 GAE/100 g) compared to HMK (431.0 GAE/100 g). TPC was 8% higher in ethanol extract from HMBK (541.9 GAE/100 g) compared to HMK (499.9 GAE/100 g). DPPH radical scavenging activity was 1.6 times higher in the methanol extracts from HMBK than HMK. In contrast there was no difference in DPPH radical scavenging activity between HMBK and HMK. Oxygen radical absorbance capacities in methanol and ethanol extracts from HMBK were similar to HMK, but both were higher than extracts from FPK (55% and 23% higher, respectively). Inhibition of angiotensin I converting enzyme activity in methanol extracts from HMBK was similar to HMK, but it was 2.6 times higher than inhibition activities from FPK. Interestingly, only ethanol extract from HMBK showed a 10.7% and 18.3% inhibition on cell growth of the human colon adenocarcinoma grade II cell line (HT-29) and human lung carcinoma cell line (NCI-H1229), respectively. These results indicate home-made Kochujang with broccoli leaf powder contains high total phenolics, antioxidant activities, and cancer cell growth inhibition activities.

기능성 고추장을 제조할 목적으로 브로콜리잎 분말을 첨가하여 고추장을 제조한 후 80% 메탄올, 80% 에탄올, 물로 추출하여 총페놀 함량과 항산화 및 항암활성을 측정하여 재래식 고추장 및 개량식 고추장과 비교하였다. 총페놀 함량은 80% 메탄올과 80% 에탄올 추출물에서 브로콜리 고추장이 각각 524.2와 541.9 mg GAE/100 g으로 재래식 고추장의 431.0과 499.9 mg GAE/100 g보다 각각 22%와 8% 높았다. DPPH radical 소거능은 80% 메탄올 추출물에서는 브로콜리 고추장이 재래식 고추장보다 1.6배 높았으나, 80% 에탄올 추출물에서는 브로콜리 고추장과 재래식 고추장이 비슷하였다. 활성산소 흡수능력은 80% 메탄올과 80% 에탄올 추출물에서 브로콜리 고추장이 재래식 고추장보다 높았으나 유의적인 차이를 나타내지 않았으며 개량식 고추장보다는 각각 55%와 23% 높았다. ACE 저해 활성은 80% 메탄올 추출물에서 브로콜리 고추장은 재래식 고추장과는 유사하였으나 개량식 고추장보다는 2.6배 높았다. 항암활성은 브로콜리 고추장의 80% 에탄올 추출물인 경우 대장암 세포주(HT-29)와 폐암 세포주(NCI-H1229)에서 각각 10.7과 18.3%의 생장 저해효과를 나타내었다. 따라서 브로콜리잎의 건조분말을 첨가하여 재래식 방법으로 제조한 고추장은 총페놀 함량이 높으며, 재래식 고추장이나 공장식 고추장보다 다소 높은 항산화 활성과 암세포 성장 저해효과를 나타내어 브로콜리 분말의 첨가에 의해 고추장의 기능성이 향상되었다.

Keywords

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