Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Protective Effect of Canavalia gladiata on Gastric Inflammation Induced by Alcohol Treatment in Rats

알코올성 위염 동물 모델에서 작두콩 추출물의 보호 효과

  • Kim, Ok Kyung (Dept. of Medical Nutrition, Kyung Hee University) ;
  • Nam, Da-Eun (Dept. of Medical Nutrition, Kyung Hee University) ;
  • You, Yanghee (Dept. of Food and Nutrition, Chonnam National University) ;
  • Jun, Woojin (Dept. of Food and Nutrition, Chonnam National University) ;
  • Lee, Jeongmin (Dept. of Medical Nutrition, Kyung Hee University)
  • 김옥경 (경희대학교 의학영양학과) ;
  • 남다은 (경희대학교 의학영양학과) ;
  • 유양희 (전남대학교 식품영양학과) ;
  • 전우진 (전남대학교 식품영양학과) ;
  • 이정민 (경희대학교 의학영양학과)
  • Received : 2013.01.28
  • Accepted : 2013.03.27
  • Published : 2013.05.31
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Abstract

The objective of this study was to investigate the protective effect of extracts from Canavalia gladiata (CGE) on gastric inflammation induced by alcohol treatment in SD rats. Rats were divided into four groups: G1 (normal group), G2 (gastric inflammation induced by alcohol), G3 (gastric inflammation induced by alcohol with lansoprazole pretreatment), G4 (gastric inflammation induced by alcohol with 250 mg/kg b.w. CGE pretreatment), G5 (gastric inflammation induced by alcohol with 500 mg/kg b.w. CGE pretreatment). After the oral administration of 40% alcohol and samples for seven days, acute gastritis was induced with 70% alcohol and 0.15 M HCl. After 1 h of alcohol administration, the animals were sacrificed. Groups pretreated with lansoprazole or CGE showed an attenuation of gastric mucosal injury, including decreases in sub-epithelial loss, hemorrhages, and gastric juice secretion induced by administration of alcohol. The oral administration of CGE (500 mg/kg b.w.) significantly decreased the levels of TBARS. To examine molecular factors that regulate inflammation, the protein expression of NF-${\kappa}B$ and COX-2 were measured through immuno-histochemistry. Compared with the normal group (G1), the expression of NF-${\kappa}B$ and COX-2 were clearly increased in G2. COX-2 and NF-${\kappa}B$ were expressed even higher in groups pretreated with CGE compared to G2. In conclusion, our data show that Canavalia gladiata has inhibitory and protective effects on gastric inflammation induced by alcohol treatment in SD rats.

본 연구는 국내에서 재배되는 작두콩 추출물의 투여가 알코올 처리로 유발된 위염 동물모델에서 위 점막 손상, 위액분비, 염증성 인자 발현의 변화에 미치는 영향을 관찰한 후 위장 보호 효과의 기능성을 입증시켜 기능성 식품 개발의 기초자료를 제공하고자 하였다. 작두콩 80% 알코올 추출물을 투여한 그룹에서 알코올성 위염을 유발시킨 그룹에 비하여 위 점막 손상과 출혈이 감소하였음을 관찰하였으며 위 손상면적 또한 유의적으로 감소시켰다. 위액분비량을 비교한 결과에서도 알코올성 위염으로 인하여 증가한 위액분비량을 작두콩 80% 알코올 추출물이 농도 의존적으로 감소시켰음을 확인할 수 있었다. 위 조직의 병리학적인 관찰을 통하여 효과를 관찰한 결과 알코올성 위염을 유발한 군에서 위 점막조직의 손상과 표면 상피세포의 손실을 관찰할 수 있었으나, 작두콩 80% 알코올 추출물 250 및 500 mg/kg 투여한 군들에서는 알코올성 위염 그룹과 비교하여 손상이 깊지 않음을 확인할 수 있었다. TBARS 양으로 산화적 손상을 측정한 결과에서는 작두콩 80% 알코올 추출물 500 mg/kg을 투여한 그룹에서 알코올성 위염으로 인하여 증가된 산화적 손상을 대조물질 lansoprazole 30 mg/kg 투여군과 유의적인 차이가 없는 정도로 감소시켰음을 확인하였다. 염증 관련인자인 COX-2와 NF-${\kappa}B$의 발현을 비교한 결과, 알코올성 위염을 유발시킨 시험대조군에서 정상대조군보다 COX-2와 NF-${\kappa}B$의 발현이 확연히 증가한 반면 대조물질 lansoprazole 30 mg/kg 투여한 그룹에서는 발현이 감소하는 것을 확인할 수 있었으나 작두콩 80% 알코올 추출물을 투여한 군에서는 시험대조군에 비해 미비한 감소를 보였다. 따라서 작두콩 80% 알코올 추출물의 알코올성 위염 예방 효과는 형태학적 및 병리학적 관찰, 산화적 손상 억제, 염증억제효과 등으로 확인한 결과, 알코올성 위염을 억제시켰으며 이는 저용량 보다는 고용량을 섭취하였을 때 그 보호효과가 크다는 것을 확인하였다.

Keywords

References

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