Effect of Bee Venom Pharmacopuncture and Scolopendra Subspinipes Pharmacopuncture on Functional Recovery and Anti-inflammation after Sciatic Crushed Nerve Injury in Rats

봉독약침과 오공약침이 좌골신경 손상 흰쥐의 기능 회복과 염증 억제에 미치는 영향

  • Lee, Gill-Jae (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Ga-Chon University) ;
  • Song, Yun-Kyoung (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Ga-Chon University) ;
  • Lim, Hyung-Ho (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Ga-Chon University)
  • 이길재 (가천대학교 한의과대학 한방재활의학과교실) ;
  • 송윤경 (가천대학교 한의과대학 한방재활의학과교실) ;
  • 임형호 (가천대학교 한의과대학 한방재활의학과교실)
  • Published : 2013.04.30

Abstract

Objectives : Peripheral nerve injuries are commonly encountered clinical problems and often result in serve functional deficit. Bee venom and scolopendra subspinipes have been traditionally used in oriental medicine to treat several inflammatory diseases and chronic pain conditions. Methods : In the present study, the effects of bee venom pharmacopuncture and scolopendra subspinipes pharmacopuncture on functional recovery, severity of pain, and expressions of neurofilament, cycloxygenease-2(COX-2), and tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) following sciatic crushed nerve injury in rats were investigated. For this study, walking tract analysis, plantar test, western blot for COX-2 and TNF-${\alpha}$, and immunohistochemistry for neurofilament were performed. Results : In the present results, sciatic functional index(SFI) in walking tract analysis was significantly decreased following sciatic crushed nerve injury, and pain severity in plantar test was significantly increased. COX-2 and TNF-${\alpha}$ expressions were increased whereas neurofilament expression was decreased by sciatic crushed nerve injury. On the other hand, bee venom pharmacopuncture and scolopendra subspinipes pharmacopuncture improved SFI in walking tract analysis and suppressed the pain severity in sciatic crushed nerve injury. Bee venom pharmacopuncture and scolopendra subspinipes pharmacopuncture suppressed COX-2 and TNF-${\alpha}$ expression and enhanced the neurofilament expression in sciatic crushed nerve injury. Conclusions : In the present study, we have shown that treatment with bee venom or scolopendra subspinipes is the effective therapeutic modality to ameliorate the symptoms of sciatic crushed nerve injury. The efficacies of bee venom and scolopendra subspinipes were similar.

Keywords

References

  1. 김상범, 배성한, 어경윤. 외상성 말초신경 손상에 대한 임상적 고찰. 동국의학. 1993;1:43-50.
  2. Kline DG, Kim D, Midha R, Harsh C, Tiel R. Management and results of sciatic nerve injuries a 24-year experience. J Neurosurg. 1998;89:13-23. https://doi.org/10.3171/jns.1998.89.1.0013
  3. Cornwall R, Radomisli TE. Nerve injury in traumatic dislocation of the hip. Clin Orthop. 2000;377:84-91. https://doi.org/10.1097/00003086-200008000-00012
  4. Hyun JK, Lee SJ, Yoo DS, Park HG, Kwon BS. The electrodiagnostic finding of sciatic nerve injury according the locations and etiologies. J Korean Acad Rehabil Med. 2004;28:54-8.
  5. Leung L, Cahill CM. TNF-alpha and neuropathic pain-a review. J Neuroinflammation. 2010;16:7-27.
  6. Baron R. Neuropathic pain. The long path from mechanisms to mechanism-based treatment. Der Anaesthesist. 2000;49:373-86. https://doi.org/10.1007/s001010070105
  7. 이동현, 윤대환, 나창수, 윤여충. 환도혈 봉독약침 자극이 백서의 신경병리성 동통 억제에 미치는 영향. 대한침구학회지. 2005;22:67-77.
  8. 김성남, 김성철, 최회강, 소기숙, 임정아, 황우준, 문형철, 최성용, 이상관, 나창수. 環跳穴 蜈蚣藥鍼 刺戟이 白鼠의 神經病理性 痛症 抑制에 미치는 影響. 대한침구학회지. 2004;21:145-67.
  9. 김선종, 정문재, 박성혁, 김정환, 염승룡, 권영달, 송용선. 白屈菜 藥鍼이 白鼠의 末梢 神經病症性 痛症 抑制에 미치는 影響. 한방재활의학과학회지. 2007;17:1-16.
  10. 김지영, 고형균, 김용석, 박영배, 김창환, 강성길. 蜂毒療法의 最新 硏究動向에 對한 考察. 대한침구학회지. 1997;14:47-71.
  11. 이상훈, 이현종, 백용현, 김수영, 박재경, 홍승재, 양형인, 김건식, 이재동, 최도영, 이두익, 이윤호. 봉독약침이 류마티스 관절염 환자의 관절 통증, 종창 및 급성 염증 반응에 미치는 영향. 대한침구학회지. 2003;20:77-84.
  12. 왕오호, 안규범, 임진강, 장형석. 退行性 膝關節炎의 蜂毒藥鍼 治療效果에 대한 臨床的 觀察. 대한침구학회지. 2001;18:35-47.
  13. 김태희, 강계성, 권기록. 蜂藥鍼療法을 이용한 股關節病變 治驗 症例報告. 대한약침학회지. 2001;4:127-34.
  14. 권기록, 고형균. 봉약침요법의 면역반응에 관한 임상적 연구. 대한침구학회지. 2000;17:169-74.
  15. 정경숙, 김수현, 박선경, 임호제, 윤형선, 안호진, 임호재. 뇌졸중 후 중추성 통증 환자에 대한 봉독약침요법 약침 치료 효과에 대한 임상적 연구. 대한침구학회지. 2005;22:69-75.
  16. 대한약침학회. 약침제재와 임상응용(I). 서울:대한약침학회 출판부. 1997:227.
  17. 송춘호, 장경전, 정병태, 안창범. 蜈蚣水鍼이 鎭痛 및 鎭痙效果에 미치는 影響. 대한침구학회지. 1997;14:219-30.
  18. 이삼로, 김성철, 구성태, 김성남, 황우준, 이건목, 조남근, 임규상. 白鼠에서 蜈蚣藥鍼이 神經病症性 痛症에 미치는 影響. 대한경락경혈학회지. 2004;21:47-67.
  19. 이동희, 김호철, 안덕균. 蜈蚣의 항고혈압작용에 관한 연구. 대한본초학회지. 1997;1239-49.
  20. 김길섭, 서운교, 정지천. 蜈蚣이 老齡에 따른 mouse의 免疫機能에 미치는 影響. 대한한방내과학회지. 1998;19:477-87.
  21. 최용건, 이동웅, 김근우, 구병수. 蜈蚣의 항산화 효과에 관한 연구. 동의신경정신과학회지. 2008;19:129-42.
  22. 고형균, 인창식. 동의수의침구학. 서울:경희대학교출판국. 2003:34-40, 320.
  23. Bain JR, Mackinnon SE, Hunter DA. Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat. Plast Reconstr Surg. 1989;83:129-38. https://doi.org/10.1097/00006534-198901000-00024
  24. Pavic R, Pavic ML, Tvrdeic A, Tot OK, Heffer M. Rat sciatic nerve crush injury and recovery tracked by plantar test and immunohistochemistry analysis. Coll Antropol. 2011;35:93-100.
  25. Kemp SW, Walsh SK, Midha R. Growth factor and stem cell enhanced conduits in peripheral nerve regeneration and repair. Neurol Res. 2008;30:1030-8. https://doi.org/10.1179/174313208X362505
  26. Delmotte A, Rigoard S, Buffenoir K, Wager M, Giot JP, Robert R, Lapierre F, Rigoard P. Physiology of the injured peripheral nerve. Neurochirurgie. 2009;1:13-21.
  27. Irintchev A. Potentials and limitations of peripheral nerve injury models in rodents with particular reference to the femoral nerve. Ann Anat. 2011;193:276-85. https://doi.org/10.1016/j.aanat.2011.02.019
  28. Klusakova I, Dubovy P. Experimental models of peripheral neuropathic pain based on traumatic nerve injuries-an anatomical perspective. Ann Anat. 2009;191:248-59. https://doi.org/10.1016/j.aanat.2009.02.007
  29. 대한약침학회 학술위원회. 약침학. 서울:엘스비어코리아. 2008:6-30.
  30. Ameratunga RV, Hawkins R, Prestidge R, Marbrook J. A high efficiency method for purification and assay of bee venom phospholipase A2. Pathology. 1995;27:157-60. https://doi.org/10.1080/00313029500169782
  31. Rybak-Chmielewska H, Szezesna T. HPLC study of chemical composition of honeybee venom. J Agric Sci. 2004;48:103-9.
  32. Kokot ZJ, Matysiak J. Simultaneous determination of major constituents of honeybee venom by LC-DAD. Chromatographia. 2009;69:1-5.
  33. Kwon YB, Lee HJ, Han HJ, Mar WC, Kang SK, Yoon OB, Beitz AJ, Lee JH. The water soluble fraction of bee venom produces antinociceptive and antiinflammatory effects on rheumatoid arthritis in rats. Life Sci. 2002;71:191-204. https://doi.org/10.1016/S0024-3205(02)01617-X
  34. 안중철, 권기록, 이승배, 임태진, 이은희, 차배천. 봉독과 sweet bee venom의 항균 및 항산화능 비교연구. 대한약침학회지. 2006;21:97-104.
  35. Tu WC, Wu CC, Hsieh HL, Chen CY, Hsu SL. Honeybee venom induces calcium-dependent but caspase-independent apoptotic cell death in human melanoma A2058 cells. Toxicon. 2008;52:318-29. https://doi.org/10.1016/j.toxicon.2008.06.007
  36. 정혜윤, 고형균. 蜂毒 藥鍼液이 炎症 및 痛症關聯 遺傳子 發現에 미치는 影響. 대한침구학회지. 2002;19:41-50.
  37. 한상미, 이광길, 여주홍, 권해용, 우순옥, 이명렬, 이만영, 김선여, 백하주, 박관규. 국내산 봉독에 의한 BV-2 세포주에서 Nitric Oxide와 Tumor Necrocis Factor-${\alpha}$ 생성에 미치는 항신경염증 작용에 관한 연구. 한국양봉학회지. 2005;20:95-102.
  38. 조일주, 박성주, 송호준, 최미옥, 박민철. 마우스 대식세포주인 RAW 264.7 세포에서 蜈蚣의 항염증 효과. 대한본초학회지. 2011;26:23-9.
  39. 김창민, 신민교, 이경순, 안덕균. 중약대사전. 서울:정담. 1998:3912-23.
  40. 전국한의과대학 본초학 교수. 본초학. 서울:영림사. 2000:508-9.
  41. 고강훈, 박소영, 최회강, 소기숙, 황우준, 이건목, 김성철. 蜈蚣藥針을 이용한 腰椎間板脫出症治療의 臨床的 硏究. 대한약침학회지. 2002;4:47-57.
  42. 김성남, 김성철, 최회강, 소기숙, 임정아, 문형철, 이종덕, 최성용, 김홍훈, 이옥자. 腰椎間板脫出形態別 蜈蚣藥鍼의 效果에 관한 臨床的 硏究. 대한침구학회지. 2004;21:79-99.
  43. 전국한의과대학 침구경혈학교실. 침구학(상). 서울:집문당. 1998:643-5.
  44. 나영분. 수혈학. 상해:상해과학기술출판사. 1998:186-7.
  45. Dellon AL, Mackinnon SE. Sciatic nerve regeneration in the rat. Validity of walking track assessment in the presence of chronic contractures. Microsurgery. 1989;10:220-5. https://doi.org/10.1002/micr.1920100316
  46. Fawcett JW, Keynes RJ. Peripheral nerve regeneration. Annu Rev Neurosci. 1990;13:43-60. https://doi.org/10.1146/annurev.ne.13.030190.000355
  47. Fawcett JW, Rosser AE, Dunnett SB. Peripheral nerve regeneration in Brain damage, Brain repair. NY:Oxford University Press. 2001:145-54.
  48. Akassoglou K, Yu WM, Akpinar P, Strickland S. Fibrin inhibits peripheral nerve regeneration by arresting Schwann cell differentiation. Neuron. 2002;33:861-75. https://doi.org/10.1016/S0896-6273(02)00617-7
  49. 은영준, 송윤경, 임형호. 해인탕 추출물이 흰쥐 좌골신경 손상 모델에서 기능회복과 뇌의 c-Fos 발현에 미치는 영향. 한방재활의학과학회지. 2011;21:125-42.
  50. Schwager J, Schulze J. Modulation of interleukin production by ascorbic acid. Vet Immunol Immunopathol. 1998;64:45-57. https://doi.org/10.1016/S0165-2427(98)00120-2
  51. Durrenberger PF, Facer P, Gray RA, Chessell IP, Naylor A, Bountra C, Banati RB, Birch R, Anand P. Cyclooxygenase-2 in injured human nerve and a rat model of nerve injury. J Peripher Nerv Syst. 2004;9:15-25. https://doi.org/10.1111/j.1085-9489.2004.09104.x
  52. Schafers M, Marziniak M, Sorkin LS, Yaksh TL, Sommer C. Cyclooxygenase inhibition in nerve-injury- and TNF-induced hyperalgesia in the rat. Exp Neurol. 2004;185:160-8. https://doi.org/10.1016/j.expneurol.2003.09.015