Asian-Australasian Journal of Animal Sciences

  • 제26권7호
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  • Pages.921-929
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  • 2013
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Gene Expression Profiling in the Pituitary Gland of Laying Period and Ceased Period Huoyan Geese

  • Luan, Xinhong (College of Animal Science and Veterinary Medicine, Shenyang Agricultural University) ;
  • Cao, Zhongzan (College of Animal Science and Veterinary Medicine, Shenyang Agricultural University) ;
  • Xu, Wen (College of Animal Science and Veterinary Medicine, Shenyang Agricultural University) ;
  • Gao, Ming (College of Animal Science and Veterinary Medicine, Shenyang Agricultural University) ;
  • Wang, Laiyou (Liaoning Province Livestock and Poultry Genetic Resources Conservation and Utilization Center) ;
  • Zhang, Shuwei (Liaoning Province Livestock and Poultry Genetic Resources Conservation and Utilization Center)
  • 투고 : 2013.02.01
  • 심사 : 2013.03.22
  • 발행 : 2013.07.01
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초록

Huoyan goose is a Chinese local breed famous for its higher laying performance, but the problems of variety degeneration have emerged recently, especially a decrease in the number of eggs laid. In order to better understand the molecular mechanism that underlies egg laying in Huoyan geese, gene profiles in the pituitary gland of Huoyan geese taken during the laying period and ceased period were investigated using the suppression subtractive hybridization (SSH) method. Total RNA was extracted from pituitary glands of ceased period and laying period geese. The cDNA in the pituitary glands of ceased geese was subtracted from the cDNA in the pituitary glands of laying geese (forward subtraction); the reverse subtraction was also performed. After sequencing and annotation, a total of 30 and 24 up and down-regulated genes were obtained from the forward and reverse SSH libraries, respectively. These genes mostly related to biosynthetic process, cellular nitrogen compound metabolic process, transport, cell differentiation, cellular protein modification process, signal transduction, small molecule metabolic process. Furthermore, eleven genes were selected for further analyses by quantitative real-time PCR (qRT-PCR). The qRT-PCR results for the most part were consistent with the SSH results. Among these genes, Synaptotagmin-1 (SYT1) and Stathmin-2 (STMN2) were substantially over-expressed in laying period compared to ceased period. These results could serve as an important reference for elucidating the molecular mechanism of higher laying performance in Huoyan geese.

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피인용 문헌

  1. Transcriptome Profiling Identifies Differentially Expressed Genes in Huoyan Goose Ovaries between the Laying Period and Ceased Period vol.9, pp.11, 2014, https://doi.org/10.1371/journal.pone.0113211
  2. Transcriptome profiling of the hypothalamus during prelaying and laying periods in Sichuan white geese (Anser cygnoides) vol.86, pp.8, 2015, https://doi.org/10.1111/asj.12356
  3. Comparative proteomic analysis of pituitary glands from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach vol.12, pp.9, 2017, https://doi.org/10.1371/journal.pone.0185253
  4. Comparative proteomic analysis of hypothalamus tissue from Huoyan geese between pre-laying period and laying period using an iTRAQ-based approach vol.89, pp.7, 2018, https://doi.org/10.1111/asj.13012
  5. Molecular cloning and expression analysis of the Synaptotagmin-1 gene in the hypothalamus and pituitary of Huoyan goose during different stages of the egg-laying cycle vol.12, pp.None, 2014, https://doi.org/10.1186/1477-7827-12-83