Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Detection Characteristics of Gamma-Irradiated Seeds by using PSL, TL, ESR and GC/MS

PSL, TL, ESR 및 GC/MS 분석을 통한 감마선 조사된 유지종실류의 검지 특성 연구

  • Kim, Kyu-Heon (New Hazardous Substances Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Son, Jin-Hyok (Hazardous Substances Analysis Division, Seoul Regional Food and Drug Administration) ;
  • Kang, Yoon-Jung (Hazardous Substances Analysis Team, Busan Regional Food and Drug Administration) ;
  • Park, Hye-Young (Imported Food Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Kwak, Ji-Young (New Hazardous Substances Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Lee, Jae-Hwang (New Hazardous Substances Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Park, Yong-Chjun (New Hazardous Substances Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Jo, Tae-Yong (Hazardous Substances Analysis Team, Busan Regional Food and Drug Administration) ;
  • Kim, Jae-I (New Hazardous Substances Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Lee, Hwa-Jung (New Hazardous Substances Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Lee, Sang-Jae (New Hazardous Substances Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Han, Sang-Bae (New Hazardous Substances Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
  • 김규헌 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 손진혁 (서울지방식품의약품안전청 유해물질분석과) ;
  • 강윤정 (부산지방식품의약품안전청 유해물질분석팀) ;
  • 박혜영 (경인지방식품의약품안전청 수입식품분석팀) ;
  • 곽지영 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 이재황 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 박용춘 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 조태용 (부산지방식품의약품안전청 유해물질분석팀) ;
  • 김재이 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 이화정 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 이상재 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 한상배 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀)
  • Received : 2013.03.11
  • Accepted : 2013.04.15
  • Published : 2013.06.30
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Abstract

In this study, we investigated the applicability of the photostimulated luminescence (PSL), thermoluminescence (TL), electron spin resonance (ESR) and gas chromatography/mass spectrometry (GC/MS) methods for 5 seeds which are not allowed to be irradiated in Korea. All 5 seeds including evening primrose seed, safflower seed, rape seed, sunflower seed and flax seed were analyzed. Samples were irradiated at 1~10 kGy using a $^{60}Co$ gamma-ray irradiator. In PSL study, the photon counts of all the unirradiated samples showed negative (lower than 700). The photon counts of irradiated (1, 5, 10 kGy) samples showed positive (higher than 5,000). In TL analysis, results showed that it is possible to apply TL method to all foods containing minerals. In ESR measurements, the ESR signal (single-line) intensity of irradiated foods was higher than non-irradiated foods. The hydrocarbons 1,7-hexadecadiene ($C_{16:2}$) and 8-heptadecene ($C_{17:1}$) from oleic acid were detected only in the irradiated samples before and after the treatment at doses ${\geq}$ 1 kGy, but they were not detected in non-irradiated samples before and after treatment. These two hydrocarbons could be used as markers to identify irradiated safflower seed, rape seed, Sunflower seed and flax seed. And then, the hydrocarbons 1,7,10-hexadecatriene ($C_{16:3}$) and 6,9-heptadecadiene ($C_{17:2}$) from linoleic acid were detected in the evening primrose seed, safflower seed and sunflower seed. According to the results, PSL, TL and GC/MS methods were successfully applied to detect the irradiated foods. It is concluded that PSL, TL and GC/MS methods are suitable for detection of irradiated samples and a combined method is recommendable for enhancing the reliability of detection results.

본 연구는 비허용 품목인 유지종실류 5종(달맞이꽃씨, 홍화씨, 유채씨, 해바라기씨 및 아마씨)을 선정하여 광자극발광법(PSL), 열발광법(TL), 전자스핀공명법(ESR) 및 기체크로마토그래프/질량분석법(GC/MS)을 활용하여 검지 특성 연구를 수행하였다. PSL 및 TL 측정 결과, 5종 모두 적용 가능성이 높은 것으로 판단하였다. ESR 분석결과는 5종 모두 조사시료에서 조사유래의 특이 ESR signal이 관찰되지 않아 적용 가능성이 낮다고 판단하였다. GC/MS 분석 결과, 홍화씨, 유채씨, 해바라기씨 및 아마씨의 경우에는 조사 유래의 hydrocarbon류가 검출되어 적용 가능성이 높은 것으로 판단하였다. 특히, oleic acid에서 유도된 8-heptadecene ($C_{17:1}$), 1,7-hexadecadiene ($C_{16:2}$)이 조사 여부를 확인하는 화학적 마커로 활용 가능성이 높았다. 달맞이꽃씨 경우에는 oleic acid에서 유도된 hydrocarbon류가 검출되지 않아 적용 가능성이 낮다고 판단하였다. 또한, 달맞이꽃씨, 홍화씨 및 해바라기씨에서 감마리놀렌산의 주요 원료인 linoleic acid에서 유도된 1,7,10-hexadecatriene ($C_{16:3}$)과 6,9-heptadecadiene ($C_{16:3}$)이 검출되어 조사여부를 확인하는 화학적 검지 마커로 활용 가능성이 높다고 판단하였다. 본 연구 결과를 통해 유지종실류 5종에 대한 적용 가능성 및 다중분석 체계(PSL-TL-GC/MS)를 확립하였다.

Keywords

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