Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Development of RAPD-Derived SCAR Markers and Multiplex-PCR for Authentication of the Schisandrae Fructus

오미자 (五味子) 종 감별을 위한 RAPD 유래 SCAR Marker 및 Multiplex-PCR 기법 개발

  • Lee, Young Mi (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong Cheol (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Ji, Yunui (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Seo, Hyeong Seok (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Ho Kyoung (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
  • 이영미 (한국한의학연구원 한약기초연구그룹) ;
  • 문병철 (한국한의학연구원 한약기초연구그룹) ;
  • 지윤의 (한국한의학연구원 한약기초연구그룹) ;
  • 서형석 (한국한의학연구원 한약기초연구그룹) ;
  • 김호경 (한국한의학연구원 한약기초연구그룹)
  • Received : 2013.04.04
  • Accepted : 2013.04.22
  • Published : 2013.06.30
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Abstract

The fruits of Schisandra chinensis have been used as an edible ingredient and traditional medicine in Korea. Due to morphological similarities of dried mature fruits, the correct identification of S. chinensis from other closely related Schisandrae species is very difficult. Therefore, molecular biological tools based on genetic analysis are required to identify authentic Schisandrae Fructus. Random amplifed polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop an easy, reliable and reproducible method for the authentication of these four species. In this paper, we developed several RAPD-derived species specific SCAR markers and established a multiplex-PCR condition suitable to discriminate each species. These genetic markers will be useful to distinguish and authenticate Schisandrae Fructus and four medicinal plants, S. chinensis, S. sphenanthera, S. repanda and K. japonica, in species level.

Keywords

References

  1. Ao CQ. (2006). Morphological characters of leaf epidermis in Schisandraceae and their systematic significance. Journal of Plant Biology. 49:80-87. https://doi.org/10.1007/BF03030791
  2. China Pharmacopoeia Committee. (2010). Pharmacopoeia of the People's Republic of China. China Medical Science Press. Beijing, China. p.61, p.227.
  3. Cho YJ, Ju IS, Kim BC, Lee WS, Kim MJ, Lee BG, An BJ, Kim JH and Kwon OJ. (2007). Biological activity of Omija(Schizandra cinensis Baillon) extracts. Journal of the Korean Society for Applied Biological Chemistry. 50:198-203.
  4. Choo BK, Moon BC, Ji Y, Kim BB, Choi G, Yoon T and Kim HK. (2009). Development of SCAR markers for the discrimination of three species of medicinal plants, Angelica decursiva(Peucedanum decursivum), Peucedanum praeruptorum and Anthricus sylvestris, based on the internal transcribed spacer(ITS) sequence and random amplified polymorphic DNA(RAPD). Biological & Pharmaceutical Bulletin. 32:24-30. https://doi.org/10.1248/bpb.32.24
  5. Choi SR, Kim CS, Kim JY, You DH, Kim JM, Kim YS. Song EJ, Kim YG, Ahn YS and Choi DG. (2011). Changes of antioxidant activity and lignan contents in Schisandra chinensis by harvesting times. Korean Journal of Medicinal Crop Science. 19:414-420. https://doi.org/10.7783/KJMCS.2011.19.6.414
  6. Gao JP, Wang YH, Qiao CF and Chen DF. (2003). Ribosomal DNA its sequences analysis of the chinese crude drug Fructus Schisandrae Sphenantherae and fruits of Schisandra viridis. Zhnghuo Zhong Yao Za Zhi. 28:706-710.
  7. Jeong SY. (2006). Studies on the establishment of guide to authenticate herbal drugs on morphologically. A Korea Food & Drug Administration Research Paper. p.144-147.
  8. Kim JS, Jang HW, Kim JS, Kim HJ and Kim JH. (2012). Molecular identification of Schisandra chinensis and its allied species using multiplex PCR based on SNPs. Genes & Genomics. 34:283-290. https://doi.org/10.1007/s13258-011-0201-3
  9. Kim KS, Prak CG and Bang JK. (2003). Varietal and yearly differences of lignan contents in fruits of collected lines of Schizandra chinensis Baillon. Korean Journal of Medicinal Crop Science. 11:71-75.
  10. Kim SH, Lee KY, Baik ES, Han J and Kang MS. (2010). Morphology and chlorophyll contents of leaf and wood anatomical characteristics of three Schisandraceae species in Korea. Korean Journal of Plant Resources. 23:31-37.
  11. Kiran U, Khan S, Mirza KJ, Ram M and Abdin MZ. (2010). SCAR marker: A potential tool for authentication of herbal drugs. Fitoterapia. 81:969-976. https://doi.org/10.1016/j.fitote.2010.08.002
  12. Korea Food & Drug Administration(KFDA). (2012). The Korean pharmacopoeia(10th Ed.). Korea Food & Drug Administration. Chungwon, Korea. p.76-77.
  13. Lee CB. (1979). Coloured flora of Korea. Hyangmoonsa. Seoul, Korea. p.375-376.
  14. Moon BC, Lee YM, Chun JM, Lee AY, Yoon T, Cheon MS, Choo BK and Kim HK. (2009). Development of SCAR markers for the discrimination of Rhei radix et Rhizoma and Rhei undulatai Rhizoma based on the RAPD. Korean Journal of Herbology. 24:115-120.
  15. Moon BC, Ji Y, Seo HS, Lee AY, Chun JM and Kim HK. (2010). Molecular authentication of Schisandrae Fructus and analysis of phylogenetic relationship based on nrDNA-its sequences. Korean Journal of Herbology. 25:47-54.
  16. Moon BC, Ji Y, Lee YM, Chun JM, Lee AY, Choo BK and Kim HK. (2011). Development of SCAR markers for authentication of Acori Rhizoma based on the analysis of RAPD and multiplex-PCR. Korean Journal of Medicinal Crop Science. 19:162-169. https://doi.org/10.7783/KJMCS.2011.19.3.162
  17. Paran I and Michlmore RW. (1993). Development of reliable PCR based markers linked to downy mildew resistance gene in lettuce. Theoretical and Applied Genetics. 85:985-993.
  18. Park CG, Bang KH, Kim OT, Jin DC and Kim DH. (2007). Development of SCAR marker for discriminating between violet flowered lines and white flowered lines in Chinese bellflower(Platycodon grandiflorum A.). Korean Journal of Medicinal Crop Science. 15:1-5.
  19. Sucher MJ and Carles MC. (2008). Genome-based approaches to the authentication of medicinal plants. Planta Medica. 74:603-623. https://doi.org/10.1055/s-2008-1074517
  20. Sun Y, Liu YF and Huang HW. (2009). Isolation and characterization of polymorphic microsatellite markers in Schisandra chinensis(Turcz.) Baill.(Schisandraceae). Conservation Genetics Resources. 1:119-121. https://doi.org/10.1007/s12686-009-9029-4
  21. Whang WK. (2003). Studies on the preparation of crude drug (III). A Korea Food & Drug Administration Research Paper. p.43-46.
  22. Yan B, Wang J, Chen G and Wang T. (2009). Isolation and characterization of polymorphic microsatellite loci in a traditional Chinese medicinal plant, Schisandra sphenanthera. Conservation Genetics Resources. 10:615-617. https://doi.org/10.1007/s10592-008-9590-5

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