Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Enzyme Inhibition Activities of Ethanol Extracts from Germinating Rough Rice (Oryza sativar L.)

발아기간에 따른 벼(Oryza sativa L.)의 부위별 효소저해활성

  • Kim, Min Young (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Sang Hoon (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jang, Gwi Young (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Park, Hye Jin (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Meishan, Li (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kim, Shinje (Center for Fungi and Plant Genome Research, FnP Corp.) ;
  • Lee, Youn Ri (Dept. of Food and Nutrition, Daejeon Health Sciences College) ;
  • Lee, Junsoo (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • Received : 2013.02.26
  • Accepted : 2013.04.24
  • Published : 2013.06.30
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Abstract

This study investigated the enzyme inhibitory effects of ethanol extracts from the different parts of rough rice (Oryza sativar L.) from its germination period. Rough rice was germinated at $37^{\circ}C$ for 6 days, then separated into hull+sprout and brown rice. ${\alpha}$-Glucosidase inhibitory activity had the highest value (39.38%) in hull+sprout extracts after 5 days of germination. ${\alpha}$-Amylase and DPP-4 inhibitory activity had the highest values (75.32% and 47.77%, respectively) in hull+sprout extracts after germination for 5 days. ACE inhibitory activity of hull+ sprout extracts increased from 43.16% at the beginning to 58.60% at 5 days, while brown rice extracts increased this activity from 0.88% at the beginning to 14.50% at 4 days. The xanthine oxidase inhibitory activity of hull+ sprout extracts increased from 62.02% at the beginning to 64.49% at one day, and then decreased. Lipase inhibitory activity had its highest value (55.17%) in hull+sprout extracts after germination for 5 days. These results indicate that the optimal germination period for increasing enzyme inhibitory activities may be 5 days, and that hull+sprout extracts have a higher enzyme inhibition activity than brown rice.

본 연구에서는 발아기간 및 부위별 벼 추출물에 대한 다양한 효소활성 저해효과를 조사하였다. 발아기간은 6일까지로 하였고, 발아된 벼는 왕겨+싹 부위와 현미로 나누어 80% ethanol로 환류 추출하였다. ${\alpha}$-Glucosidase 저해활성은 왕겨+싹 추출물에서 발아 전 26.32%에서 발아 5일차 39.38%로 증가하였으며, ${\alpha}$-amylase 저해활성은 왕겨+싹 추출물에서 발아 전 59.98%에서 발아 5일차 75.32%로 증가하였고, DPP-4 저해활성 또한 왕겨+싹 추출물에서 발아 전 38.80%에서 발아 5일차 47.77%로 증가하여 발아에 의해 효소활성 저해가 증가하였다. ACE 저해활성은 왕겨+싹 추출물에서 가장 높았으며 발아에 의해 증가하였다. Xanthine oxidase 저해활성은 발아가 진행됨에 따라 감소하였다. Lipase 저해활성은 발아 4일차 왕겨+싹 추출물이 36.78%로 무발아 31.72%에 비하여 증가하였다. 본 연구결과 벼를 발아시킬 경우 왕겨+싹에서 성인병 관료 효소를 억제하는 성분이 증가함을 알 수 있었으며, 추후에 어떠한 성분에 의한 것인지에 대한 연구가 수행되어야 할 것으로 판단된다.

Keywords

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