Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activities of Processed Deoduck (Codonopsis lanceolata) Extracts

가공공정에 따른 더덕 추출물의 항산화 활성

  • Jeon, Sang-Min (Functional Food and Nutrition Division, Department of Agrofood Resources, Rural Development Administration) ;
  • Kim, So-Young (Functional Food and Nutrition Division, Department of Agrofood Resources, Rural Development Administration) ;
  • Kim, In-Hye (Functional Food and Nutrition Division, Department of Agrofood Resources, Rural Development Administration) ;
  • Go, Jeong-Sook (Functional Food and Nutrition Division, Department of Agrofood Resources, Rural Development Administration) ;
  • Kim, Haeng-Ran (Functional Food and Nutrition Division, Department of Agrofood Resources, Rural Development Administration) ;
  • Jeong, Jae-Youn (New Tree Co., Ltd.) ;
  • Lee, Hyeon-Yong (Dept. of Teaics, Seowon University) ;
  • Park, Dong-Sik (Functional Food and Nutrition Division, Department of Agrofood Resources, Rural Development Administration)
  • 전상민 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김소영 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김인혜 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 고정숙 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김행란 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 정재윤 ((주)뉴트리) ;
  • 이현용 (서원대학교 차학과) ;
  • 박동식 (농촌진흥청 국립농업과학원 기능성식품과)
  • Received : 2013.02.05
  • Accepted : 2013.02.27
  • Published : 2013.06.30
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Abstract

This study investigated the antioxidant activities of processed Deoduck (Codonopsis lanceolata) extracts treated through high-pressure extraction and steaming with fermentation. The antioxidant activities were determined for DPPH and ABTS radical-scavenging activity, SOD-like activity, ferric reducing antioxidant power (FRAP), and $Fe^{2+}$ chelating. Total phenolic and flavonoid contents were also measured. Among eight Deoduck extracts, the S5FDW extract had the highest total phenolic and flavonoid content, 73.9 mg GAE/g and 50.9 mg QUE/g, respectively. The S5FDW extract had the highest DPPH radical-scavenging activity (27%) at a 1.0 mg/mL concentration. The ABTS radical-scavenging activity was highest for S5FDW extract (82.1%) at a 10 mg/mL concentration. The HFDE extract showed the highest SOD-like activity (29.7%) at a 1.0 mg/mL concentration. FRAP was highest in S5FDW extract (140.8 ${\mu}M$) at a 1.0 mg/mL concentration. The DE extract showed the highest $Fe^{2+}$ chelating (46%) at a 1.0 mg/mL concentration. The phenolic and flavonoid contents significantly correlated with the antioxidant activity of several processed Deoduck extracts and was higher in the processed Deoduck extracts compared to the raw Deoduck extracts. Therefore, processing techniques can be useful methods for making Deoduck a more potent and natural antioxidant.

본 연구에서는 다양한 가공공정 처리를 한 더덕 추출물의 총 폴리페놀 및 플라보노이드 함량과 항산화활성 변화를 비교하였다. 총 폴리페놀 및 플라보노이드 함량은 S5FDW에서 73.9 mg GAE/g과 50.9 mg QUE/g으로 가장 높게 나타났으며, DE에서는 15.7 mg GAE/g과 14.7 mg QUE/g으로 가장 낮았다. DPPH와 ABTS radical 소거능은 농도 의존적인 경향을 나타냈으며, 1.0 mg/mL 농도에서 $27.0{\pm}2.5%$, $17.4{\pm}0.6%$로 S5FEW에서 가장 높은 소거능을 보였다. SOD 유사활성은 1.0 mg/mL 농도에서 HFDE가 $29.7{\pm}3.2%$로 높은 유사활성을 보였으며, DE가 $19.9{\pm}1.6%$로 낮은 활성을 나타내었다. FRAP에 의한 환원력은 S5FDW가 1.0 mg/mL 농도에서 $140.8{\pm}4.5$ ${\mu}M$ Fe(II)/g으로 높은 환원력을 나타내었다. $Fe^{2+}$ chelating 활성은 1.0 mg/mL 농도에서 $46.0{\pm}1.6%$로 DE가 높은 활성을 보였고, HFDE가 $15.8{\pm}1.0%$로 낮은 활성을 나타내었다. 가공공정을 이용한 더덕 추출물의 폴리페놀 및 플라보노이드 함량과 항산화 활성 간의 상관관계는 총 폴리페놀 함량과 총 플라보노이드, DPPH 및 ABTS radical 소거능 그리고 FRAP에 의한 환원력은 r=0.958(p<0.001), r=0.849(p<0.01), r=0.932(p<0.01) 및 r=0.985(p<0.001)로 높은 양의 상관관계를 나타내었다. 총 플라보노이드 함량과 DPPH 및 ABTS radical 소거능과 FRAP에 의한 환원력은 r=0.818(p<0.05), r=0.834(p<0.05) 및 r=0.944(p<0.001)로 유의적인 상관관계를 나타내어 폴리페놀 및 플라보노이드 함량이 항산화에 중요한 영향을 미치는 것으로 사료된다. 이상의 결과, 더덕의 가공공정은 더덕의 물리적 조직 변화와 더덕 성분의 화학적 변화를 일으켜 페놀성 물질을 증가시키므로 항산화 성분 함량과 항산화 활성 증가를 가져오는 것으로 사료된다. 따라서 이러한 가공공정을 거친 더덕을 활용하여 항산화 활성이 증가된 천연 항산화제 개발이 가능할 것이며, 향후 가공과정 중 증가 또는 생성되는 활성물질에 대한 구조와 작용메커니즘 등에 대한 추가적인 연구가 필요하다고 생각한다.

Keywords

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