Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Comparison of Toxicity and Deposition of Nano-Sized Carbon Black Aerosol Prepared With or Without Dispersing Sonication

  • Kang, Mingu (Center for Chemicals Safety and Health Research, Occupational Safety and Health Research Institute) ;
  • Lim, Cheol-Hong (Center for Chemicals Safety and Health Research, Occupational Safety and Health Research Institute) ;
  • Han, Jeong-Hee (Center for Chemicals Safety and Health Research, Occupational Safety and Health Research Institute)
  • Received : 2013.05.28
  • Accepted : 2013.06.26
  • Published : 2013.06.30
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Abstract

Nanotoxicological research has shown toxicity of nanomaterials to be inversely related to particle size. However, the contribution of agglomeration to the toxicity of nanomaterials has not been sufficiently studied, although it is known that agglomeration is associated with increased nanomaterial size. In this study, we prepared aerosols of nano-sized carbon black by 2 different ways to verify the effects of agglomeration on the toxicity and deposition of nano-sized carbon black. The 2 methods of preparation included the carbon black dispersion method that facilitated clustering without sonication and the carbon black dispersion method involving sonication to achieve scattering and deagglomeration. Male Sprague-Dawley rats were exposed to carbon black aerosols 6 hr a day for 3 days or for 2 weeks. The median mass aerodynamic diameter of carbon black aerosols averaged $2.08{\mu}m$ (for aerosol prepared without sonication; group N) and $1.79{\mu}m$ (for aerosol prepared without sonication; group S). The average concentration of carbon black during the exposure period for group N and group S was $13.08{\pm}3.18mg/m^3$ and $13.67{\pm}3.54mg/m^3$, respectively, in the 3-day experiment. The average concentration during the 2-week experiment was $9.83{\pm}3.42mg/m^3$ and $9.08{\pm}4.49mg/m^3$ for group N and group S, respectively. The amount of carbon black deposition in the lungs was significantly higher in group S than in group N in both 3-day and 2-week experiments. The number of total cells, macrophages and polymorphonuclear leukocytes in the bronchoalveolar lavage (BAL) fluid, and the number of total white blood cells and neutrophils in the blood in the 2-week experiment were significantly higher in group S than in normal control. However, differences were not found in the inflammatory cytokine levels (IL-$1{\beta}$, TNF-${\alpha}$, IL-6, etc.) and protein indicators of cell damage (albumin and lactate dehydrogenase) in the BAL fluid of both group N and group S as compared to the normal control. In conclusion, carbon black aerosol generated by sonication possesses smaller nanoparticles that are deposited to a greater extent in the lungs than is aerosol formulated without sonication. Additionally, rats were narrowly more affected when exposed to carbon black aerosol generated by sonication as compared to that produced without sonication.

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References

  1. NSET. (2010) The National nanotechnology Initiative. Research and Development Leading to a Revolution in Technology and Industry Supplement to the Present's FY 2011 Budget. Subcommittee on nanoscale Science, Engineering and Technology, Committee on Technology, National Science and Technology Council, Washington, DC, pp. 1-65.
  2. Ferin, J., Oberdoster, G., Penney, D.P., Soderholm, S.C., Gelein, R. and Piper, H.C. (1990) Increased pulmonary toxicity of ultrafine particles 1. Particle clearance, translocation, morphology. J. Aerosol Sci., 21, 381-384. https://doi.org/10.1016/0021-8502(90)90064-5
  3. Oberdorster, G., Ferin, J., Finkelstein, G., Wade, P. and Corson, N. (1990) Increased pulmonary toxicity of ultrafine particles. 2. Lung lavage studies. J. Aerosol Sci., 21, 384-387. https://doi.org/10.1016/0021-8502(90)90065-6
  4. Maynard, A.D., Warheit, D.B. and Philbert, M.A. (2011) The new toxicology of sophisticated materials: Nanotoxicology and beyond. Toxicol. Sci., 120, S109-S129. https://doi.org/10.1093/toxsci/kfq372
  5. Madl, A.K. and Pinkerton, K.E. (2009) Health effects of inhaled engineered and incidental nanoparticles. Crit. Rev. Toxicol., 39, 629-658. https://doi.org/10.1080/10408440903133788
  6. Castranova, V. (2011) Overview of current toxicological knowledge of engineered nanoparticles. J. Occup. Environ. Med., 53, S14-S17. https://doi.org/10.1097/JOM.0b013e31821b1e5a
  7. Zook, J.M., MacCuspie, R.I., Locascio, L.E., Halter, M.D. and Elliott, J.T. (2011) Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity. Nanotoxicology, 5, 517-530. https://doi.org/10.3109/17435390.2010.536615
  8. Stefaniak, A.B., Turk, G.C., Dickerson, R.M. and Hoover, M.D. (2008) Size-selective poorly soluble particulate reference materials for evaluation of quantitative analytical methods. Anal. Bioanal. Chem., 391, 2071-2077. https://doi.org/10.1007/s00216-008-1870-x
  9. Kleinstreuer, C., Zhang, Z. and Kim, C.S. (2007) Combined inertial and gravitational deposition of microparticles in small model airways of a human respiratory system. J. Aerosol Sci., 38, 1047-1061. https://doi.org/10.1016/j.jaerosci.2007.08.010
  10. OECD. (2012) Guidance on sample preparation and dosimetry for the safety testing of manufactured nanomaterials, OECD Environment, Health and Safety Publications, Paris, pp. 69-93.
  11. Lim, C.H., Kang, M., Han, J.H. and Yang, J.S. (2012) Effect of agglomeration on the toxicity of nano-sized carbon black in Sprague-Dawley rats. Environ. Health Toxicol., 27, e2012015. https://doi.org/10.5620/eht.2012.27.e2012015
  12. International Carbon Black Association. (2012) What is Carbon Black, Hebei LongHao Chemical Technology Co., LTD, Shandong, pp. 1.
  13. Porter, D., Sriram, K., Wolfarth, M., Jefferson, A., Schwegler- Berry, D., Andrew, M.E. and Castranova, V. (2008) A biocompatible medium for nanoparticle dispersion. Nanotoxicology, 2, 144-154. https://doi.org/10.1080/17435390802318349
  14. Kim, S.C., Chen, D.R., Qi, C., Gelein, R.M., Finkelstein, J.N., Elder, A., Bentley, K., Oberdörster, G. and Pui, D.Y. (2010) A nanoparticle dispersion method for in vitro and in vivo nanotoxicity study. Nanotoxicology, 4, 42-51. https://doi.org/10.3109/17435390903374019
  15. Sager, T.M., Porter, D.W., Robinson, V.A., Lindsley, W.G., Schwegler-Berry, D.E. and Castranova, V. (2007) Improved method to disperse nanoparticles for in vitro and in vivo investigation of toxicity. Nanotoxicology, 1, 118-129. https://doi.org/10.1080/17435390701381596
  16. Bihari, P., Vippola, M., Schultes, S., Praetner, M., Khandoga, A.G., Reichel, C.A., Coester, C., Tuomi, T., Reberg, M. and Krombach, F. (2008) Optimized dispersion of nanoparticles for biological in vitro and in vivo studies. Part. Fibre Toxicol., 5, 14. https://doi.org/10.1186/1743-8977-5-14
  17. Driscoll, K.E., Carter, J.M., Howard, B.W., Hassenbein, D.G., Pepelko, W., Baggs, R.B. and Oberdorster, G. (1996) Pulmonary inflammatory, chemokine, and mutagenic responses in rats after subchronic inhalation of carbon black. Toxicol. Appl. Pharmacol., 136, 372-380. https://doi.org/10.1006/taap.1996.0045
  18. Elder, A., Gelein, R., Finkelstein, J.N., Driscoll, K.E., Harkema, J. and Oberdorster, G. (2005) Effects of subchronically inhaled carbon black in three species. 1. Retention kinetics, lung inflammation, and histopathology. Toxicol Sci., 88, 614-629. https://doi.org/10.1093/toxsci/kfi327
  19. Carter, J.M., Corson, N., Driscoll, K.E., Elder, A., Finkelstein, J.N., Harkema, J.N., Gelein, R., Wade-Mercer, P., Nguyen, K. and Oberdorster, G. (2006) A comparative doserelated response of several key pro- and antiinflammatory mediators in the lungs of rats, mice, and hamsters after subchronic inhalation of carbon black. J. Occup. Environ. Med., 48, 1265-1278. https://doi.org/10.1097/01.jom.0000230489.06025.14

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