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Studies on the Grain Quality Characteristics of Rice Transgenic Lines

벼 형질전환계통의 미질특성에 대한 고찰

  • 정종민 (국립식량과학원 춘천출장소) ;
  • 정지웅 (국립식량과학원 답작과) ;
  • 강경호 (국립식량과학원 답작과) ;
  • 이상복 (국립식량과학원 춘천출장소) ;
  • 모영준 (국립식량과학원 답작과) ;
  • 김정곤 (국립식량과학원 답작과) ;
  • 김경민 (경북대학교 응용생명과학부) ;
  • 손재근 (경북대학교 응용생명과학부)
  • Received : 2013.04.22
  • Accepted : 2013.05.17
  • Published : 2013.06.30

Abstract

Selected 11 rice transgenic lines, through preliminary yield trials on 32 lines, were evaluated on important agronomic traits as well as grain quality by conducting replicated yield trials having three replication plots. Japonica recipients, Nipponbare, Nagdongbyeo, and Dongjinbyeo, were the recipients of 7 transformed genes, which were putatively related with high yield, abiotic stress tolerance, and disease resistance. To estimate the degrees of deviations from the wild types, 11 traits of transgenic lines, relating with grain quality were evaluated and subjected to multivariate analyses. Principal coordinate and clustering analyses did not support collection manners of transgenic lines in terms of the genes transformed as well as the genetic background. Meanwhile, some transgenic lines would be acceptable due to their over-all performances were similar to their wild types, it was hardly possible to declare any transgenic line, which adhered closely to the commercial profits of wild type. Thereby, with considerations on the demanding resources in establishing rice transgenic lines having market competitiveness, it was speculated that proper application of breeding strategies would be crucial factor for the efficiency of developing prospective rice transgenic lines.

국내 대학과 국립식량과학원 간의 협력 연구를 통해 확보된 32개 벼 형질전환 계통들 중 작물학적 성능이 유망한 11개 계통들에 대해 작물학적 특성과 미질 특성이 평가되었다. 주요 결과는 아래와 같다. 1. 이들 계통들은 수량성, 환경스트레스저항성, 내병성에 관여하는 7종의 유전자가 니폰바레, 낙동벼, 동진벼 등에 형질전환 되어 육성된 계통들이다. 2. 미질 특성과 관련된 11개 조사형질을 이용한 다변량 분석결과, 형질전환에 이용된 모품종과 도입유전자들에 대한 각 형질전환 계통군들의 뚜렷한 집구현상은 관찰되지 않았다. 3. 실용적 측면에서는 작물학적 특성이 모품종과 유사하면서 미질 특성이 양호한 계통이 다수 확인되었다. 4. 그러나 모품종의 유용한 작물학적 특성, 즉 '상품성'을 온전히 견지하고 있다고 판단되는 계통은 선정하기 어려웠다. 5. 도입할 유전자의 선정과 유전적으로 고정된 형질전환 계통을 확보하는데 소요되는 경비와 시간을 고려할 때, 향후 우량 벼 형질전환 계통의 육성효율을 제고하기 위해서는 기존에 비해 보다 구체적이며 차별화 된 육종전략의 개발이 필요하다고 판단되었다.

Keywords

References

  1. Biosafety White Paper. 2007. Ministry of commerce, Industry and Energy, Korea research institute of bioscience and biotechnology. I Will. pp. 206-216.
  2. James, C. 2011. Global Status of Commercialized Biotech /GM Crops. ISAAA Brief No. 43. ISAAA: Ithaca, NY.
  3. Juliano, B. O. 1971. Simplified assay for milled rice amylose. Cereal Sci. Today 16 : 344-340.
  4. Juliano, B. O. 1985. Criteria and tests for rice grain qualities. Chemistry and technology, AACC. pp. 443-524.
  5. Kang, H. J., J. K. Lee, S. Y. Lee, S. Y. Lee, K. Y. Park, and J. U. Shim. 1995. Variation of some characters in rice protoplast-derived plants. Korean J. Breed. 27 : 298-302.
  6. Kim, K. M., Y. I. Kuk, W. Kim, W. Back, J. O. Guh, and M. S. Shin. 2006. Quality of rice lines transformed with protox gene of Bacillus subtilis. Korean J. Breed. 38: 44-50.
  7. Larkin, P. J. & W. R. Scowcroft. 1981. Somaclonal variation -a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60 : 1 97-214. https://doi.org/10.1007/BF02342540
  8. Lee, S. Y., J. K. Lee, T. H. Noh, H. J. Kang, S. Y. Lee. 1993. Variation of major characters in plant regenerated from rice cells treated with Ethyl Methane Sulfonic Acid. Korean J. Plant Tissue Culture. 20 : 307-314.
  9. Lim, S. J., H. G. Hwang, B. G. Oh, M. H. Nam, D. Y. Kwak, G. H. Yi, and N. B. Park. 1998. Variation of physicochemical characteristics of grain in rice plants derived from cell culture. RDA J. Crop Sci. 40 : 9-13.
  10. Maqbool, S. B. and P. Christou. 1999. Multiple traits of agronomic importance in transgenic indica rice plants analysis of transgene integration patterns, expression levels and stability. Molecular Breeding 5 : 471-480. https://doi.org/10.1023/A:1009634226797
  11. Park, Y. H., T. H. Kim, H. S. Lee, K. M. Kim, and J. K. Sohn. 2010 Morphological and progeny variation in somaclonal mutant of 'Ilpum'(Oryza sativa L.). Korean J. Breed 27 : 404-408.
  12. Schaeffer, G. W., F. T. Sharpe Jr, and P. B. Cregan. 1984. Variation for improved protein and yield from rice anther culture. Theor. Appl. Genet. 67 : 383-389.
  13. Shin, K. S., J. H. Park, J. H. Lee, S. M. Lee, H. J. Woo, S. H. Lim, H. Y. Kim, S. C. Suh, and S. J. Kweon. 2009. Qualitative PCR detection of stack gene GM rice (LS28${\times}$Cry1Ac) developed in Korea. J. Appl. Biol. Chem. 52 : 1-7. https://doi.org/10.3839/jabc.2009.001
  14. Sohn, J. K., O. H. Kwon, S. T. Cheong, and I. K. Rhee. 1991. Variation of agronomic characters in regenerated plants from callus culture of rice. Korean J. Breed. 23 : 181-187.
  15. Sohn, J. K., G. H. Yi, B. G. Oh, and S. J. Lim. 1995. Variation of some agronomic traits in anther-derived rice plants. Korean J. Breed 27 : 404-408.
  16. Thuc, L. V., D. Geelen, H. Ky, S. E. Ooi, S. E. Napis, U. R. Sinniah, and P. Namasivayam. 2013. Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility. Mol. Biol. Rep. 40 : 1579-1590. https://doi.org/10.1007/s11033-012-2206-7
  17. Toriyama, K., Y. Arimoto, H. Uchimiya, and K. Hinata. 1988. Transgenic rice plants after direct gene transfer into protoplasts. Bio/Technology. 6 : 1072-1074. https://doi.org/10.1038/nbt0988-1072
  18. Xie, Q. J., C. Ruch, and J. H. Oard. 1995. Homozygous variation in rice somaclones : Non random variation instead of mitotic recombination, Crop Sci. 35: 954-957. https://doi.org/10.2135/cropsci1995.0011183X003500040002x
  19. Yamagishi, M., K. Itoh, T. Koba, Y. Sukekiyo, K. Shimamoto, and T. Shimada. 1997. Characteristics of genetic variation in the progenies of protoplast-derived plants of rice. Oryza sativa cv. Nipponbare, Theor. Appl. Genet. 94 : 1-7. https://doi.org/10.1007/s001220050374
  20. Ye, X. D., Al-Babili, S. Kloti, A. Zhang, J. Lucca, P. Beyer, and I. Potrykus. 2000. Engineering the provitamin A($\beta$-carotene) biosynthetic pathway into (carotenoidfree) rice endosperm. Science. 287 : 303-305. https://doi.org/10.1126/science.287.5451.303
  21. 2011 바이오안전성백서. 2011. 한국생명공학연구원 바이오 안전성정보센터. pp. 248-250.
  22. 쌀 품질분석기술. 2008. 농촌진흥청 작물과학원 work-shop교재. 상록사.
  23. 정지웅, 강경호, 전용희, 신영섭, 최인배. 2012. 약배양과 여교잡 기술을 이용한 우량 벼 형질전환체의 단기생산법, 대한민국 특허 제 10-1212058호.