YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Antibiotic Resistant Patterns and DNA Fingerprint Analysis of Acinetobacter baumannii from Clinical Isolates

임상에서 분리된 Acinetobacter baumannii의 항생제 내성 패턴과 유전학적 특징

  • Kim, Min-Ji (Department of Pharmacy, Sahmyook University) ;
  • Lee, Si-Won (Department of Pharmacy, Sahmyook University) ;
  • Lee, Do-Kyung (Department of Pharmacy, Sahmyook University) ;
  • Park, Jae-Eun (Department of Pharmacy, Duksung Women's University) ;
  • Kang, Joo-Yeon (Department of Pharmacy, Duksung Women's University) ;
  • Park, Il-Ho (Department of Pharmacy, Sahmyook University) ;
  • Shin, Hae-Soon (Department of Pharmacy, Duksung Women's University) ;
  • Ha, Nam-Joo (Department of Pharmacy, Sahmyook University)
  • Received : 2013.03.21
  • Accepted : 2013.04.01
  • Published : 2013.04.30
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Abstract

Acinetobacter baumannii is gram-negative bacilli that can be widely found in environments. Recently, A. baumannii emerged as a serious nosocomial infection. A total of 92 A. baumannii were isolated from hospitalized patients in Seoul, Korea, between December 2010 and April 2011. Antimicrobial susceptibility testing was investigated using CLSI agar dilution methods. Tigecycline non-susceptible A. baumannii isolates were investigated by repetitive extragenic palindromic sequence-based PCR (rep-PCR). Pulsed-field gel electrophoresis was performed to determine the epidemiological relationships. All clinical isolates showed high-level resistance to the most commonly used antibiotics: Ciprofloxacin (87.0%), Ampicillin/sulbactam (82.6%), Cefotaxime (81.5%), Ceftazidime (80.4%). Moreover, 50.0% of these isolates were non-susceptible to tigecycline. When evaluated by RAPD analysis, generated distinct band ranging in size from 1kb to 8k band varying from 4 to 10 bands. Stricter surveillance and more rapid detection are essential to prevent the spread of multi drug resistant A. baumannii.

Keywords

References

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