Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effects of a Herbal Preparation HJ01 on Adipocyte Differentiation in OP9 Cells and the Poloxamer-407 Induced Hyperlipidemia in Mice

HJ01이 OP9세포에서의 지방 분화와 P-407로 유발한 고지혈증 흰쥐에 미치는 영향

  • Park, Jung-Eun (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Han, Sang-Yong (Department of Herbal Medicine, College of Pharmaceutics, Wonkwang University) ;
  • Choi, Eun-Sik (Department of Herbal Medicine, College of Pharmaceutics, Wonkwang University) ;
  • Chong, Myong-Soo (Department of Pre-Herb Medicine, College of Korean medicine, Wonkwang University) ;
  • Kim, Yun-Kyung (Department of Herbal Medicine, College of Pharmaceutics, Wonkwang University)
  • 박정은 (원광대학교 한의학전문대학원 제3의학과) ;
  • 한상용 (원광대학교 약학대학 한약학과 방제학교실) ;
  • 최은식 (원광대학교 약학대학 한약학과 방제학교실) ;
  • 정명수 (원광대학교 한의과대학 한의예과) ;
  • 김윤경 (원광대학교 약학대학 한약학과 방제학교실)
  • Received : 2013.06.04
  • Accepted : 2013.06.12
  • Published : 2013.06.30
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Abstract

Objectives : This study was designed to investigate the effect of a herbal preparation HJ01 consisting of Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus and Glycyrrhizae Radix on adipocyte differentiation in OP9 cells and on poloxamer 407(P-407)-induced hyperlipidemia in mice. Methods : 1. MTT assay was used to evaluate the potential cytotoxicity of Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus, Glycyrrhizae Radix and HJ01, respectively. 2. Bone-marrow derived OP9 cells were treated with HJ01, and the alterations in fat storage in the cells were determined by the Oil red O assay. 3. The protein level of CAAAT/enhancer binding protein alpha($C/EBP{\alpha}$), as a adipocyte differentiation marker, was examined using western blot analysis in differentiated OP6 cells. 4. Adult male C57BL6 mice received intraperitoneal injections of P407 to induce hyperlipidemia, simultaneously, were treated with HJ01 for 4 weeks. Then the cholesterol (TC), triglyceride (TG) and high-density lipoprotein-cholesterol (HDL-c) levels in sera and liver tissues were measured. Results : 1. The MTT assay exhibited that Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus, Glycyrrhizae Radix and HJ01 showed no significant cytotoxicity in tested dosages. 2. Ten days' treatment with HJ01 markedly inhibited the increases in fat storage in differentiated OP6 cells. 3. Four weeks' treatment with HJ01 down-regulated the protein level of CAAAT/enhancer binding protein alpha($C/EBP{\alpha}$) but up-regulated the levels of adiponectin in differentiated OP9 cells. 5. HJ01 inhibited the accumulation of TC and TG in liver tissues and increased serum levels of TC in hyperlipidemic mice. Conclusions : These results suggest that HJ01 can in vitro inhibit adipocyte differentiation and fat storage in OP6 cells, in vivo improve the hyperlipidemia induced by P-407 in mice, which may be mediated by promoting glucose uptake and improving a lipid metabolite profile.

Keywords

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