Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Inhibitory Effect of Rhein on Renal Fibrosis in Diabetic Nephropathy Rats

대황산(大黃酸)의 당뇨병쥐 신장조직섬유화 억제 효과에 관한 실험연구

  • Zhao, Rongjie (Department of Pharmacology Mudanjiang Medical University) ;
  • Zhao, Zhenglin (Department of Biochemistry Mudanjiang Medical University) ;
  • Zhang, Jie (Department of Biochemistry Mudanjiang Medical University) ;
  • Liu, Hongfeng (Department of Biochemistry Mudanjiang Medical University) ;
  • Cui, Rongjun (Department of Biochemistry Mudanjiang Medical University) ;
  • Kim, Sang Chan (College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Sun-Hyung (College of Oriental Medicine, Dongguk University)
  • 조용걸 (모단강의학원 약리학교실) ;
  • 조정림 (모단강의학원 생화학교실) ;
  • 장걸 (모단강의학원 생화학교실) ;
  • 류홍봉 (모단강의학원 생화학교실) ;
  • 최영군 (모단강의학원 생화학교실) ;
  • 김상찬 (대구한의대학교 방제학교실) ;
  • 김선형 (동국대학교 한의과대학)
  • Received : 2013.06.03
  • Accepted : 2013.06.10
  • Published : 2013.06.30
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Abstract

Objectives : To investigate the therapeutic effect and underlying mechanisms of rhein on renal fibrosis in diabetic rats. Methods : Diabetic nephropathy (DN) was induced in adult Wistar rats via introperitoneal injection of streptozotocin (STZ) (20 mg/kg/d) for three consecutive days. Two days after the last dose of STZ, rhein was administered to the diabetic rats at a dose of 25 mg/kg or 50 mg/kg, twice a day by gavage, respectively. Following 28 days treatment with rhein, the plasma glucose and creatinine levels were measured, the renal levels of TGF-${\beta}1$ protein and mRNA were examined, and the fibronectin mRNA levels were also determined. Results : Rhein significantly inhibited the increased plasma glucose and creatinine levels of diabetic rats in a dose- and a time-dependent way. Immunohistochemical analysis showed both doses of rhein markedly attenuated elevated induction of renal TGF-${\beta}1$ protein expressions in diabetic rats. Additionally, the high dose of rhein improved both TGF-${\beta}1$ and fibronectin mRNA expressions, while the low dose of rhein only alleviated fibronectin mRNA expressions. Conclusions : Rhein can improve renal fibrosis in diabetic nephropathy rats, and which may be mediated through inhibition of the renal mRNA expressions of TGF-${\beta}1$ and fibronectin.

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References

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