Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Repeated-dose oral toxicity study of crude antifungal compounds produced by Lactobacillus plantarum AF1 in rats

Lactobacillus plantarum AF1이 생성한 조항진균 물질의 흰쥐에 대한 반복투여독성

  • 이환 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과) ;
  • 장해춘 (조선대학교 식품영양학과) ;
  • 이재준 (조선대학교 식품영양학과)
  • Received : 2013.03.04
  • Accepted : 2013.06.07
  • Published : 2013.06.30
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Abstract

This study was performed to investigate the four-week repeated-dose toxicity of the crude antifungal compounds produced by Lactobacillus plantarum AF1 (Lb. plantarum AF1), a lactic acid bacterium isolated from kimchi, in male and female rats. Sprague-Dawley male and female rats were divided into four groups, with 10 animals in each group. The test article was administered once daily by gavage to rats at dosage levels of 0, 500, 1,000, and 2,000 mg/kg/day for four weeks. There were no test-article-related deaths or abnormal clinical signs in both the male and female rats during the observation period. Furthermore, no differences in the body weight changes, food intake and water consumption levels of the control and treatment groups were found. The hematological parameters, serum biochemical analysis results, histopathological examination results and all other findings also showed no significant or dose-dependent changes. There were also no changes in the organ weights upon the administration of the crude antifungal compounds produced by Lb. plantarum AF1. These results suggest that the oral administration of the crude antifungal compounds produced by Lb. plantarum AF1 had no adverse effects up to a dosage level of 2,000 mg/kg in both male and female rats.

본 연구는 김치산막효모억제 유산균인 Lb. plantarum AF1이 생산한 조항진균 물질 부분 정제물을 SD 계통의 흰쥐에게 4주간 반복 경구투여를 통하여 장기투여에 의한 안전성을 확인하였다. 암 수 흰쥐에 Lb. plantarum AF1이 생산한 조항진균 물질 부분 정제물을 0, 500, 1,000 및 2,000 mg/kg/day의 용량으로 4주간 반복 경구투여한 후 사망률, 일반증상, 체중변화, 사료섭취량, 수분섭취량, 부검소견, 장기무게 변화, 혈액학적, 혈액생화학적 및 병리조직학적 검사를 실시하였다. 모든 시험군에서 전 시험기간 시험물질로 인한 임상증상 및 사망동물이 관찰되지 않았다. Lb. plantarum AF1이 생산한 조항진균 물질 부분 정제물의 경구투여 결과 체중이 4주간 지속적으로 증가되었지만 대조군과 유의성 있는 변화가 없었다. 또한 장기의 육안적 관찰, 장기 중량변화, 혈액학적, 혈액생화학적 및 병리조직학적 검사에서도 모든 시험물질 투여군이 대조군과 유의성이 있는 차이를 보이지 않았으며, 모두 정상 범위의 수치로 시험물질에 기인하는 이상 소견을 발견할 수 없었다. 이상의 결과 4주 반복투여 독성시험 결과Lb. plantarum AF1이 생산한 조항진균 물질 부분 정제물은 저독성의 안전한 물질로 판정되었다.

Keywords

References

  1. Stiles ME (1996) Biopreservation by lactic acid bacteria. Antonie van Leewenhoek, 70, 331-345 https://doi.org/10.1007/BF00395940
  2. Messens W, de Vuyst L (2002) Inhibitory substances produced by Lactobacillus isolated sourdoughs - a review. Int J Food Microbiol, 72, 31-43 https://doi.org/10.1016/S0168-1605(01)00611-0
  3. Klaenhammer TR (1988) Bacteriocin of lactic acid bacteria. Biochim, 70, 337-349 https://doi.org/10.1016/0300-9084(88)90206-4
  4. Jack RW, Tagg JR, Ray B (1995) Bacteriocin of gram-positive bacteria. Microbiol Rev, 59, 171-200
  5. Riley MA, Wertz J (2002) Bacteriocins: evolution, ecology and application. Ann Rev Microbiol, 56, 117-137 https://doi.org/10.1146/annurev.micro.56.012302.161024
  6. Tagg JR, Dajani AS, Wannamaker LW (1976) Bacteriocins of gram-positive bacteria. Bacteriol Rev, 40, 722-756
  7. Drider D, Fimland G, Hechard Y, McMullen LH, Prevost H (2006) The continuing story of class II a bacteriocins. Microbiol Mol Biol Rev, 70, 564-582 https://doi.org/10.1128/MMBR.00016-05
  8. Lee SG, Han KS, Jeong SG, Oh MH, Jang AR, Kim DH, Bae IH, Ham JS (2010) A study on the sensory characteristic of yogurt and antimicrobial activity of Lactobacillus plantarum LHC52 isolated form kimchi. Korean J Food Sci Ani Resour, 30, 328-335 https://doi.org/10.5851/kosfa.2010.30.2.328
  9. Lee SG, Lee YJ, Kim MK, Han KS, Jeong SG, Oh MH, Jang A, Kim DH, Bae IH, Ham JS (2010) A study on the yogurt manufacture suitability and antimicrobial activity of Lactobacillus plantarum LHB55 isolated form kimchi. J Anim Sci Technol, 52, 141-148 https://doi.org/10.5187/JAST.2010.52.2.141
  10. Lim SM, Im DS (2007) Bacterial effect of bacteriocin of Lactobacillus plantarum K11 isolated from dongchimi on Escherichia coil O157. J Fd Hyg Safety. 22, 151-158
  11. Yang EJ, Chang HC (2008) Antifungal activity of Lactobacillus plantarum isolated from Kimchi. Korean J Microbiol Biotechnol, 36, 276-284
  12. Yang EJ (2008) Application of kimchi lactic acid bacteria and Bacillus subtilis for development of novel antibiotics and edible vaccine. PhD Thesis, Chosun University, Korea. p 67-69
  13. Reid G, Jass J, Sebulsky MT, McCormick JK (2003) Potential uses of probiotics in clinical practice. Clin Microbiol Rev, 16, 658-672 https://doi.org/10.1128/CMR.16.4.658-672.2003
  14. Ishibashi N, Yamazaki S (2001) Probiotics and safety. Am J Clin Nutr, 73, 465S-470S
  15. Donohue DC, Deighton M, Ahokas JT, Salminen S (1993) Toxicity of lactic acid bacteria. In: Salminen S, von Wright A. (Eds.) Lactic acid bacteria Marcell Dekker. NY, USA, p 307-313
  16. Huang Y, Kotula L, Adams MC (2003) The in vivo assessment of safety and gastrointestinal survival of an orally administrated novel probiotics, Propionibacterium jensenii 702, in a male star rat model. Food Chem Toxicol, 41, 1781-1787 https://doi.org/10.1016/S0278-6915(03)00215-1
  17. Ozlem O, Fadime K, Ingolf FN (2011) A probiotic bacterium, Pediococcus pentasaceus OZF, isolated from human breast milk products AcH/PA-1. Afr J Food Sci, 10, 2070-2079
  18. Lee H, Lee JJ, Chang HC, Lee MY (2012) Acute toxicity of Lactobacillus plantarum AF1 isolated from kimchi in mice. Korean J Food Preserv, 19, 315-321 https://doi.org/10.11002/kjfp.2012.19.2.315
  19. Lee JJ, Kim AR, Chang HC, Lee MY (2012) Repeated-dose toxicity study of Lactobacillus plantarum AF1 isolated from kimchi in rats. J Korean Soc Food Sci Nutr, 41, 612-620 https://doi.org/10.3746/jkfn.2012.41.5.612
  20. Korea Food and Drug Administration (2005) Toxicity test guidelines for safety evaluation of drugs. Notification. p 60
  21. Korea Food and Drug Administration (2005) Good laboratory practice regulation for nonclinical laboratory studies, Notification No. 2005-79. Toxicity test guidelines for safety evaluation of drugs. p 406-408
  22. Organization for economic cooperation and Development (OECD) (2001) OECD Guideline for the testing of chemicals, acute oral toxicity; Up-down-Procedure (UDP). p 425
  23. Yang EJ, Chang HC (2010) Purification of a new antifungal compound produced by Lactobacillus plantarum AF1 isolated from kimchi. Int J Food Microbiol, 139, 56-63 https://doi.org/10.1016/j.ijfoodmicro.2010.02.012
  24. Kwon JY, Cheigh HS, Song YO (2004) Weight reduction and lipid lowering effects of kimchi lactic acid powder in rats fed high fat diets. Korean J Food Sci Technol, 36, 1014-1019
  25. Cha SD, Yu JJW, Kim TW, Cho HS, Lee DH (2012) Effects of Lactobacillus plantarum CIB 001 on lipid metabolism of hypercholesterolemic rats. Korean J Food Sci Technol, 44, 324-330 https://doi.org/10.9721/KJFST.2012.44.3.324
  26. Hong CO, Seo MY, Koo YC, Nam MH, Lee HA, Kim JH, Wang Z, Yang SY, Lee SH, No SH, Lee KW (2009) Single and 14-day repeated oral toxicity studies of 70% ethanol extract of Lindera obtusiloba blume leaves. J Korean Soc Food Sci Nutr, 38, 1324-1330 https://doi.org/10.3746/jkfn.2009.38.10.1324
  27. Hayes AW (1984) Hayes toxicology. Raven press, NY, USA. p 17-19
  28. De Vuyst L, Vandamme EJ (1994) Nisin, a lantibiotic produced by Lactococcus lactis subsp. lactis: properties, biosynthesis, fermentation and application. p 152-221. In De Vuyst L, Vandamme EJ. (Eds.) Bacteriocins of lactic acid bacteria. Blackie Academic and Professional, London, UK
  29. Lorke DA (1983) New approach to practical toxicity testing. Arch Toxicol, 54, 275-287 https://doi.org/10.1007/BF01234480
  30. Iriwin S (1968) Comprehensive observational assessment: Ia. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse. Psychopharmacol, 13, 222-257 https://doi.org/10.1007/BF00401402
  31. Lee SG, Han KS, Jeong SG, Oh MH, Jang AR, Kim DH, Bae IH, Ham JS (2010) A study on the sensory characteristic of yogurt and antimicrobial activity of Lactobacillus plantarum LHC52 isolated form kimchi. Korean J Food Sci Ani Resour, 30, 328-335 https://doi.org/10.5851/kosfa.2010.30.2.328
  32. An IJ, Kwon JK, Lee JS, Lee SH, Park YS, Park BK, Kim SK, Kim BS, Cho SD, Choi CS, Lee BH, Kang BK, Jung JY (2012) Evaluate a repeated oral dose toxicity and immunomodulating activity of Pulsatilla koreana and Artemisiae annuae in sprague-dawely rats. J Fd Hyg Safety. 27, 96-102 https://doi.org/10.13103/JFHS.2012.27.1.096
  33. Kang BH, Kim YB, Lee HS, Kim YH, Im WJ, Ha CS (2004) Background data on hematology, blood biochemistry and organ weights for 2 weeks and 4 weeks repeated-dose toxicity studies using sprague-dawley (SD) rats. Korean J Lab Ani Sci, 20, 134-140
  34. Plaa GL, Charbonneau M (1994) Detection and evaluation of chemically induced liver injury. In principles and methods of toxicology. Hayes AW, ed. Raven Press, NY, USA, p 839-870
  35. Lim SS, Kim MH, Lee JH (1997) Effect of Artemisia princeps var orientalis and Circium japonicum var ussuriense on liver function body lipid and bile acid of hyperlipidemic rat. Korean J Nutr, 30, 797-80
  36. Wolford ST, Schroer RA, Cohs FX (1986) Reference range data base for serum chemistry and hematology values in laboratory animals. J Tox Env Health, 18, 161-188 https://doi.org/10.1080/15287398609530859
  37. Hata T, Tanka R, Ohmomo S (2010) Isolation and characterization of plantaricin ASM1: A new bacteriocin produced by Lactobacillus plantarum A-1. Int J Food Microbiol, 137, 94-99 https://doi.org/10.1016/j.ijfoodmicro.2009.10.021
  38. Hurst A (1981) Nisin. Adv Appl Microbiol, 27, 85-123 https://doi.org/10.1016/S0065-2164(08)70342-3

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