한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제41권1호
  • /
  • Pages.44-51
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  • 2013
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Corallococcus와 Myxococcus 속 점액세균 균주들에 의한 항균 물질의 생산

Production of Antimicrobial Substances by Strains of Myxobacteria Corallococcus and Myxococcus

  • 신혜진 (호서대학교 생명공학과 점액세균은행) ;
  • 윤진권 (호서대학교 생명공학과 점액세균은행) ;
  • 안동주 (호서대학교 생명공학과 점액세균은행) ;
  • 조경연 (호서대학교 생명공학과 점액세균은행)
  • Shin, Hyejin (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Youn, Jinkwon (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • An, Dongju (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Cho, Kyungyun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
  • 투고 : 2012.10.29
  • 심사 : 2013.01.03
  • 발행 : 2013.03.28
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초록

국내 토양시료에서 분리한 174 Corallococcus 속 균주와 207 Myxococcus 속 균주들의 배양추출물을 제조하고 Candida albicans, Pseudomonas aeruginosa, Staphylococcus aureus에 대한 항균 활성을 비교하였다. 항진균 활성을 보이는 균주의 비율은 Corallococcus가 7.5%(174균주 중 13균주)로 51.7%(207균주 중 107균주)를 보인 Myxococcus에 비해 낮았다. 하지만, 항세균 활성을 보인 균주의 비율은 Corallococcus가 12.1%(21균주)로 1%(2균주)를 보인 Myxococcus에 비해 상대적으로 높았다. Corallococcus 균주 중 P. aeruginosa, S. aureus에 모두 활성을 보이는 6 C. coralloides 균주들은 자실체 형성에서는 차이를 보였지만 항세균 활성을 나타내는 배양추출물의 HPLC 크로마토그램은 유사하였는데, 이들이 생산하는 항세균 물질의 생산은 CYS 배지에서 6일 이상 배양할 때 가장 높았다.

We prepared culture extracts of 174 Corallococcus and 207 Myxococcus strains isolated in Korea, and compared their antimicrobial activity against Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus. The percentage of strains showing antifungal activity was lower in Corallococcus (7.5% [13 of the 174 strains]) than in Myxococcus (51.7% [107 of the 207 strains]). However, the percentage of strains exhibiting antibacterial activity was higher in Corallococcus (12.1% [21 strains]) than in Myxococcus (1% [2 strains]). The culture extracts of 6 Corallococcus strains inhibited both P. aeruginosa and S. aureus and displayed similar high-performance liquid chromatography chromatograms, although the shapes of their fruiting bodies were dissimilar. The rate of production of antibacterial substances was the highest when the strains were cultured in CYS medium for more than 6 days.

키워드

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  8. Mutants defective in the production of encapsulin show a tan-phase-locked phenotype in Myxococcus xanthus vol.57, pp.9, 2019, https://doi.org/10.1007/s12275-019-8683-9
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  10. The σ54 system directly regulates bacterial natural product genes vol.11, pp.1, 2013, https://doi.org/10.1038/s41598-021-84057-4