Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Immunostimulating Effect of a Well-known Thai Folkloric Remedy in Breast Cancer Patients

  • Thisoda, Piengpen (Department of Pharmacology, Faculty of Medicine, Siriraj Hospital, Mahidol University) ;
  • Ketsa-ard, Kanchana (Department of Pharmacology, Faculty of Medicine, Siriraj Hospital, Mahidol University) ;
  • Thongprasert, Sommai (Cancer Clinic) ;
  • Vongsakul, Molvibha (Department of Microbiology, Faculty of Science, Mahidol University) ;
  • Picha, Pornthipa (Department of Research, National Cancer Institute, Ministry of Public Health) ;
  • Karbwang, Juntra (Department of Clinical Product Development, Institute of Tropical Medicine, Nagasaki University) ;
  • Na-Bangchang, Kesara (Chulabhorn International College of Medicine, Thammasat University)
  • Published : 2013.04.30
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Abstract

The study aimed to evaluate immune-stimulating effects of a well-known Thai folkloric remedy when used for adjuvant therapy with conventional chemotherapeutics for treatment of breast cancer. Immunostimulating influence of the remedy (215 mg/kg body weight per day) on NK cell activity and TNF-${\alpha}$ release from the monocytes/macrophages were investigated in a total of 15 healthy women and 13 female patients with breast cancer (Group 1). The effect of breast tumor surgery on NK cell activity was further investigated in 18 female patients with breast cancer (Group 2). NK cell cytotoxic activity was determined by chromium release cytotoxic assay using K562, an erythroleukemic cell line. TNF-${\alpha}$ release from monocytes/macrophages separated from blood samples was determined through a biological assay using actinomycin D-treated L929 mouse fibroblast cells in the presence and absence of LPS. Baseline NK cell activity of the monocytes/macrophages separated from Group 2 patients expressed as %cytotoxicity was significantly lower than in the healthy subjects at E:T ratios of 100:1 and 25:1. In healthy subjects, there was no change in NK cell cytotoxic activity (%cytotoxicity or LU) following 1 and 2 weeks of treatment with the remedy compared with the baseline at various E:T ratios but the binding activity (%binding) was significantly increased after 2 weeks of treatment. The addition of one or two conventional chemotherapeutic regimens did not significantly reduce the NK cytotoxic activity but did affect release of TNF-${\alpha}$ in both unstimulated and LPS-stimulated samples. Surgery produced a significant suppressive effect on NK cell activity. The use of the remedy as an adjunct therapy may improve therapeutic efficacy and safety profiles of conventional chemotherapeutic regimens through stimulation of the immune system in cancer patients.

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References

  1. Abe N, Ebina T, Ishida N (1982). Interferon induction by glycyrrhizin and glycyrrhetinic acid in mice. Mikroiol Immunol, 26, 535-9.
  2. Boyum A (1968). Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab, 21, 77-89. https://doi.org/10.3109/00365516809076979
  3. Burana-Osot J, Pattanapanyasat K, Soonthornchareonnon N, Sukapirom K, Toida T (2010). Characterisation and immunostimulating activity of polysaccharides from Thai medicinal plants. Nat Prod Res, 24, 1403-12. https://doi.org/10.1080/14786410902940974
  4. Cerottini JC, Brenner KT (1974). Cell mediated cytotoxicity, allograft rejection and tumor immunity. Adv Immunol, 18, 67-72. https://doi.org/10.1016/S0065-2776(08)60308-9
  5. Hiserodt JC, Britvan LJ, Targan SR (1982). Inhibition of human natural killing by heterologous and monoclonal antibodies. J Immunol, 129, 2248-54.
  6. Irwin M, Smith TL, Gillin JC (1987). Low natural killer cytotoxicity in major depression. Life Sci, 41, 2127-33. https://doi.org/10.1016/0024-3205(87)90531-5
  7. Levy S, Herberman BB, Lippman M, D'Angelo T (1987). Correlation of stress factors with sustained depression of natural killer cell activity and predicted prognosis in patients with breast cancer. J Clin Oncol, 5, 348-53.
  8. Locksley RM, Killeen N, Lenardo MJ (2001). The TNF and TNF receptor superfamilies: integrating mammalian biology. Cell, 104, 487-501. https://doi.org/10.1016/S0092-8674(01)00237-9
  9. Lozzio BB, Lozzio CB (1977). Properties of the K562 cell line derived from a patient with chronic myeloid leukemia. Int J Cancer, 19, 136-42. https://doi.org/10.1002/ijc.2910190119
  10. McCaleb B (1989). Astragalus enhance natural killer cell activity. Herbalgram, 21, 16-21.
  11. Monjazeb AM, Hsiao HH, Sckisel GD, Murphy WJ (2013). The role of antigen-specific and non-specific immunotherapy in the treatment of cancer. J Immunotoxicol, 9, 248-58.
  12. Na-Bangchang K, Wiwatwittaya S, Thongprasert S, et al (2012). Anticancer activity and immunostimulating effect on NKcell activity of a well-known Thai folkloric remedy. Int Res J Pharm Pharmacol, 2, 1-11.
  13. National Cancer Institute of Thailand. Cancer registry. http:// www.nci.go.th/Rep.htm.
  14. Pollock RE, Lotzova E (1987). Surgical-stress-related suppression of natural killer cell activity: a possible role in tumor metastasis. Nat Immun Cell Growth Regul, 6, 269-78.
  15. Pross HF, Baines MG, Rubin P, Shragge P, Patterson MS (1981) Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IX. The quantitation of natural killer cell activity. J Clin Immunol, 1, 51-63. https://doi.org/10.1007/BF00915477
  16. Ruff MR, Gifford GE (1980). Purification and physicochemical characterization of rabbit tumor necrosis factor. J Immunol, 125, 1671-7.
  17. Salagianni M, Baxevanis CN, Papamichail M, Perez SA (2012). New insights into the role of NK cells in cancer immunotherapy. Oncoimmunol, 1, 205-7. https://doi.org/10.4161/onci.1.2.18398
  18. Schmidt RE, MacDermott RP, Bartley G (1985). Specific release of proteo-glycans from human natural killer cells during target lysis. Nature, 318, 289-91. https://doi.org/10.1038/318289a0
  19. Sun Y, Hersh EM, Talapaz M (1983). Immune restoration and/or augmentation of local graft versus host reaction by traditional Chinese medicinal herbs. Cancer, 52, 70-3.
  20. Talmadge JE, Alvord WG (1987). Stress factors and breast cancer outcome. J Clin Oncol, 5, 333-4.
  21. Wagner K (1985). Immunostinulatory drugs of fungi and higher plants. In: economic and medicinal plant research Volume 1. Edited by Wagner H, Hikino H, and Farnsworth NR. New York: Academic Press, 113-53.
  22. Yamada H, Kiyohara H, Cyang JC, Kojima Y, Kumezawa Y (1984). Studies on polysaccharides from Ngelica autiloba. Planta Med, 45, 163-7.