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Comparison of Volatile Compounds of Chamaecyparis obtusa Essential Oil and its Application on the Improvement of Atopic Dermatitis

추출방법에 따른 편백 정유의 향기 성분 비교 및 아토피 개선에의 응용

  • Lim, Geum-Sook (Interdisciplinary Program of Perfume and Cosmetics, Chonnam National University Graduate School) ;
  • Kim, Ran (Department of Herb Therapy & Cosmetics, Wonkwang Health Science University) ;
  • Cho, Hoon (Department of Polymer Science & Engineering, Chosun University) ;
  • Moon, Young-Sook (Research Center for Resistant Cells, Chosun University Medical School) ;
  • Choi, Chang-Nam (Interdisciplinary Program of Perfume and Cosmetics, Chonnam National University Graduate School)
  • 임금숙 (전남대학교 일반대학원 향장품학협동과정) ;
  • 김란 (원광보건대학교 허브테라피향장과) ;
  • 조훈 (조선대학교 공과대학 응용화학소재공학과) ;
  • 문영숙 (조선대학교 의과대학 내성세포연구센터) ;
  • 최창남 (전남대학교 일반대학원 향장품학협동과정)
  • Received : 2013.02.04
  • Accepted : 2013.03.28
  • Published : 2013.04.27

Abstract

Volatile flavor compounds of Chamaecyparis obtuse essential oil were extracted by simultaneous steam distillation extraction (SDE) and supercritical fluid extraction (SFE) and analyzed by GC-MS. A total of 48 and 50 components were identified in essential oil by SDE and SFE, respectively. Monoterpenes, oxygenated monoterpenes, sesquiterpenes, oxygenated sesquiterpenes, and diterpenes in essential oil by SDE were 37.24, 10.9, 9.61, 0.22, and 0.22%, respectively. In the case of SFE, they were 19.1, 23.3, 22.66, 1.31, and 10.57%, respectively. Antioxidant activities were increased with the increase of essential oil up to $80{\mu}L/mL$, irrespective of extraction method. Especially, when the essential oil concentration extracted by SDE was increased from 20 to $80{\mu}L/mL$, the antioxidant activity was increased from 10.5 to 55.1%. However, over $80{\mu}L/mL$ of essential oil, an equilibrium state was maintained. In the case of essential oil extracted by SFE, it was decreased compared to that of SDE. For the improvement of atopic dermatitis, various cosmetics such as an ato-cide soap, ato-cide spray, and ato-cide lotion containing essential oil extracted by SFE were tested. About over 90% was useful for the improvement of atopic dermatitis after 4 weeks of clinical trial targeting 40 female adults. These results demonstrate that ato-cide soap, ato-cide spray, and ato-cide lotion containing essential oil extracted by SFE could be used in functional cosmetics.

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