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Isolation and Characterization of Ureolytic Bacteria for Biosequestration of Strontium

스트론튬 격리화를 위한 요소 분해 박테리아의 분리 및 특성 연구

  • Choi, Jae-Ho (Department of Biological Engineering, Inha University) ;
  • Kang, Chang-Ho (Department of Biological Engineering, Inha University) ;
  • Han, Sang-Hyun (Department of Biological Engineering, Inha University) ;
  • Kwak, Dae Young (Department of Biological Engineering, Inha University) ;
  • Oh, SooJi (Department of Biological Engineering, Inha University) ;
  • So, Jae-Seong (Department of Biological Engineering, Inha University)
  • Received : 2013.05.08
  • Accepted : 2013.05.28
  • Published : 2013.06.27

Abstract

In this study, 52 ureolytic bacterial strains were newly isolated from various environments. From these, 2 strains (TB-15 and TB-22) were selected based on their high urease activity. XRD spectra clearly showed presence of various sequestration products such as calcite and strontianite in samples. TB-22 showed 20~30% higher survivability upon Sr concentration (20 mM) than Sporosarcina pasteurii KCTC 3558. TB-15 and TB-22 showed 80~90% higher survivability at pH 6 than S. pasteurii. The results demonstrated that the 2 isolates colud be good candidates for the bioremediation of Sr contaminated sites.

Acknowledgement

Supported by : 한국연구재단

References

  1. Choi, B. D. (2010) Neoliberal energy policy and the limits to green growth. Korean J. Geogr. Soc. 45: 26-48.
  2. Chen, J. P. (1997) Batch and continuous adsorption of strontium by plant root tissues. Bioresour. Technol. 60: 185-189. https://doi.org/10.1016/S0960-8524(97)00021-7
  3. Chung, M. H. and O. J. Tu (1994) A study on effect of electric field for carcinogenesis of strontium 90. Korean J. Environ. Health Soc. 20: 61-77.
  4. Fujita, Y., G. D. Redden, J. C. Ingram, M. M. Cortez, F. G. Ferris, and R. W. Smith (2004) Strontium incorporation into calcite by bacterial ureolysis. Geochim. Cosmochim. Ac. 68: 3261-3270. https://doi.org/10.1016/j.gca.2003.12.018
  5. Bachmeier, K. L., A. E. Williams, J. R. Warmington, and S. S. Bang (2002) Urease activity in microbiologically-induced calcite precipitation. J. Biotechnol. 93: 171-181. https://doi.org/10.1016/S0168-1656(01)00393-5
  6. Chunxiang, Q., W. Jianyun, W. Ruixing, and C. Liang (2009) Corrosion protection of cement-based building materials by surface deposition of $CaCO_{3}$ by Bacillus pasteurii. Mater. Sci. Eng. C. 29: 1273-1280. https://doi.org/10.1016/j.msec.2008.10.025
  7. Stocks-Fisher, S., J. K. Galinat, and S. S. Bang (1999) Microbiological precipitation of $CaCO_{3}$. Soil Biol. Biochem. 31: 1563-1571. https://doi.org/10.1016/S0038-0717(99)00082-6
  8. Achal, V., X. Pan, and D. Zhang (2012) Bioremediation of strontium (Sr) contaminated aquifer quartz sand based on carbonate precipitation induced by Sr resistant Halomonas sp. Chemosphere. 89: 764-768. https://doi.org/10.1016/j.chemosphere.2012.06.064
  9. Natarajan, K. R. (1995) Kinetic study of the enzyme urease from Dolichosbiflorus. J. Chem. Educ. 72: 556-557. https://doi.org/10.1021/ed072p556
  10. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  11. Han, S. H., J. C. Lee, S. S. Jang, and P. G. Kim (2004) Composted sewage sludge can improve the physiological properties of Betula schmidtii grown in tailings J. Plant Biol. 47: 99-104.
  12. Han, S. H., S. K. Kim, C. H. Kang, J. Y. Park, J. H. Jeong, and J. S. So (2012) Environmental stress response of calcite forming bacteria isolated from concrete pavement. Korean J. Biotechnol. Bioeng. 27: 268-272.
  13. Ahmed, I., A. Yokota, A. Yamazoe, and T. Fujiwara (2007) Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov. Int. J. Syst. Evol. Microbiol. 57: 1117-1125. https://doi.org/10.1099/ijs.0.63867-0
  14. Fujita, Y., J. L. Taylor, L. M. Wendt, D. W. Reed, and R. W. Smith (2010) Evaluating the potential of native ureolytic microbes to remediate a 90Sr contaminated environment. Environ. Sci. Technol. 44: 7652-7658. https://doi.org/10.1021/es101752p
  15. Achal, V., and X. Pan (2011) Characterization of urease and carbonic anhydrase producing bacteria and their role in calcite precipitation. Curr. Microbiol. 62: 894-902. https://doi.org/10.1007/s00284-010-9801-4
  16. Ministry of Environment of Korea. https://sgis.nier.go.kr. (2013).

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