한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제42권7호
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  • Pages.1022-1028
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  • 2013
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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차가버섯 추출물에 의한 신경교모세포종 U-87 MG 세포의 증식 억제 효과

Inhibition Effect of Cell Proliferation and Apoptosis by Inonotus obliquus in Human Glioblastoma U-87 MG Cells

  • 신정아 (서울의료원 의학연구소) ;
  • 박주현 (서울의료원 의학연구소) ;
  • 김선희 (서울의료원 의학연구소) ;
  • 송관영 (서울의료원 의학연구소)
  • Shin, Jung-A (Division of Clinical Research Medical Institute, Seoul Medical Center) ;
  • Park, Joo Hyun (Division of Clinical Research Medical Institute, Seoul Medical Center) ;
  • Kim, Sun Hee (Division of Clinical Research Medical Institute, Seoul Medical Center) ;
  • Song, Kwan Yong (Division of Clinical Research Medical Institute, Seoul Medical Center)
  • 투고 : 2013.03.22
  • 심사 : 2013.05.13
  • 발행 : 2013.07.31
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초록

러시아에서 여러 민간요법으로 사용되어지고 있는 차가버섯은 대장암, 위암 등에서 항암효능이 있다고 알려져 있다. 그러나 뇌종양에 대한 연구는 현재 이루어지지 않고 있으며 차가버섯의 항암 효능에 대한 기전 연구도 미미한 실정이다. 따라서 본 연구에서는 신경교모세포종 U-87 MG 세포주에 대한 성장억제 효과를 확인하고자 세포생존율 변화와 형태학적 변화, 세포주기에 미치는 영향과 세포사멸 관련 단백질 및 세포주기 조절 단백질의 발현 변화를 조사하였다. 그 결과 차가버섯 추출물을 처리한 U-87 MG 세포 생존율이 유의하게 감소하였으며, Bcl-2, Bax와 p-p53 단백질 수준차이는 없었고 caspase-3 활성에 영향을 미쳐 죽음 수용체 경로에 의한 세포사멸이 이루어졌다. 또한 세포주기를 관찰한 결과 $G_0/G_1$ 세포주기에서 성장 억제를 확인하였으며, 이는 cyclin D1, CDK2 단백질 발현량의 감소와 RB 단백질의 인산화 억제 때문이라는 결론에 도달하였다. 본 연구결과는 차가버섯을 뇌종양의 치료 및 보조제로서 역할을 수행할 수 있을 것으로 사료된다.

Chaga mushroom (Inonotus obliquus) was considered a functional food with an anti-cancer effect in colon, gastric, and lung cancer. Therefore, this study was conducted in order to elucidate the effect of chaga mushroom extract in brain cancer. Glioblastoma U-87 MG cells were used in investigation of cell survivability, apoptosis, and cell cycle arrest analysis. Treatment with various concentrations of chaga mushroom extract resulted in inhibition of cell proliferation and cell cycle arrest. Although caspase-3 expression was increased over $100{\mu}g/mL$ of chaga mushroom extract treatment, apoptosis factors with Bcl-2, Bax and p53 did not change. In analysis of cell cycle regulatory factors, expression of cyclin D1 and CDK2 decreased in a dose-dependent manner. We have demonstrated the anti-cancer effect of chaga mushroom extract in glioblastoma, which may be mediated by activation of the caspase pathway and induction of cell cycle arrest.

키워드

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  3. Composition and biological activity of triterpenes and steroids from Inonotus obliquus (chaga) vol.62, pp.4, 2016, https://doi.org/10.18097/pbmc20166204369
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