The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Comparative Study on Anti-inflammatory Effects and Compound Contents of Multi-herbal Extracts HT008 produced by Different Manufacturing Methods

한약복합추출물 HT008의 제조방법에 따른 항염증 효능 및 성분함량 비교연구

  • Lee, Donghun (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Song, Jungbin (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Song, Mikyung (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Gaire, Bhakta Prasad (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Park, Juyeon (Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc.) ;
  • Jin, Aihong (Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc.) ;
  • Choi, Ho-Young (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Hocheol (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University)
  • 이동헌 (경희대학교 한의과대학 본초학교실) ;
  • 송정빈 (경희대학교 한의과대학 본초학교실) ;
  • 송미경 (경희대학교 한의과대학 본초학교실) ;
  • ;
  • 박주연 ((주)뉴메드 한의과학기술연구소) ;
  • 김애홍 ((주)뉴메드 한의과학기술연구소) ;
  • 최호영 (경희대학교 한의과대학 본초학교실) ;
  • 김호철 (경희대학교 한의과대학 본초학교실)
  • Received : 2013.06.24
  • Accepted : 2013.07.10
  • Published : 2013.07.30
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Abstract

Objectives : The biological activities and compound contents of herbal medicine vary depending on manufacturing processes. In this study, we compared anti-inflammatory effects and compound contents of three kinds of multi-herbal extract HT008 produced by different manufacturing processes in order to determine chemical and biological equivalence. Methods : HT008 was produced by three different manufacturing methods: 1. Freeze dried extract of Eleutherococcus senticosus, Scutellaria baicalensis and Angelica sinensis (HT008 FD), 2. Spray dried extract of E. senticosus and S. baicalensis combined with reflux extract of A. sinensis (HT008 SD), 3. Spray dried extract of E. senticosus and S. baicalensis combined with supercritial fluid extract of A. sinensis (HT008 SF). Anti-inflammatory effects were evaluated using acetic acid induced pain model and ${\lambda}$-carageenan induced paw edema model. Compound contents were evaluated by HPLC quantitative analysis of standard compounds of HT008, eleutheroside E, baicalin, z-ligustilide. Results : HT008 FD, HT008 SD and HT008 SF significantly decreased acetic acid induced pain index and ${\lambda}$-carrageenan induced paw edema volume compared with that of control group. There was no significant difference in efficacy among the HT008 FD, HT008 SD and HT008 SF. Standard compound contents of HT008 FD, HT008 SD and HT008 SF were quantified within the range of Korean pharmacopoeia or other research. Conclusions : Three different manufacturing methods of multi-herbal extracts have been developed without noticeable difference in the efficacy or compound contents. The results might be used to establish manufacturing process and industrialization of herbal extracts.

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References

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