Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Genetic Diversity of the Original Plant for Taraxaci Herba, Taraxacum spp. by the Analysis of AFLP

AFLP 분석을 통한 포공영 기원식물 민들레의 유전 다양성 분석

  • Kim, Wook Jin (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong Cheol (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Ji, Yunui (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Young Mi (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Ho Kyoung (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 김욱진 (한국한의학연구원 한약연구본부 한약기초연구그룹) ;
  • 문병철 (한국한의학연구원 한약연구본부 한약기초연구그룹) ;
  • 지윤의 (한국한의학연구원 한약연구본부 한약기초연구그룹) ;
  • 이영미 (한국한의학연구원 한약연구본부 한약기초연구그룹) ;
  • 김호경 (한국한의학연구원 한약연구본부 한약기초연구그룹)
  • Received : 2013.04.08
  • Accepted : 2013.06.17
  • Published : 2013.08.30
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Abstract

Collected germplasms of five representative dandelion species (Taraxacum ohwianum, T. platycarpum, T. platypecidum, T. officinale, and T. coreanum) were 104 lines from different habitates in Korea and China. Their genetic diversity was analyzed by genomic fingerprinting method using amplified fragment length polymorphism (AFLP). AFLP results of 6 primer combinations were revealed 1,176 total DNA fragments and 523 polymorphic bands with a 44.4% ratio of polymorphism. On the basis of similarity coefficient analysis by unweight pair group method with arithmetic averages (UPGMA), 104 dandelion germplasm lines were ranged from 0.64 to 0.99 and clustered distinct five group depending on the species. Furthermore, a principal coordinate analysis (PCA) by the application of multi-variate analysis indicated significantly greater differences among species than geographical origins.

Keywords

References

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