Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Determination of Post-harvest Fungicide in Citrus Fruits Using LC-MS

LC-MS를 이용한 감귤류의 수확 후 처리 살균제 분석

  • Choi, Su-Jeong (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Eun-Jeong (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Jae-In (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Cho, In-Soon (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Park, Won-Hee (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Hwang, In-Sook (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Moo-Sang (Gangnam Agro-marine Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Gun-Hee (Department of Food and Nutrition, Duksung Women's University)
  • 최수정 (서울시보건환경연구원 강남농수산물검사소) ;
  • 김은정 (서울시보건환경연구원 강남농수산물검사소) ;
  • 이재인 (서울시보건환경연구원 강남농수산물검사소) ;
  • 조인순 (서울시보건환경연구원 강남농수산물검사소) ;
  • 박원희 (서울시보건환경연구원 강남농수산물검사소) ;
  • 황인숙 (서울시보건환경연구원 강남농수산물검사소) ;
  • 김무상 (서울시보건환경연구원 강남농수산물검사소) ;
  • 김건희 (덕성여자대학교 식품영양학과)
  • Received : 2013.03.19
  • Accepted : 2013.05.23
  • Published : 2013.08.31
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Abstract

Post-harvest fungicide residue was measured in citrus fruits. Samples were collected from local markets in Seoul and analyzed using liquid chromatography coupled with mass spectrometry (LC-MS). LC-MS results were validated for the assay of pesticides by using linearity, accuracy, precision, and limits of detection and quantification. The linearity in the concentration ranged from 0.005 to 2.0 mg/kg ($R^2$ >0.999). Sample recoveries ranged from 80.2 to 98.3% with relative standard deviations below 4.0% for spiking levels from 0.01 to 1.0 mg/kg. The limits of detection ranged between 0.002 and 0.008 mg/kg, and the limits of quantification ranged between 0.006 and 0.027 mg/kg. The highest residue levels for carbendazim, thiabendazole, imazalil, and azoxystrobin in citrus fruits were 0.541, 0.958, 0.721, and 0.052 mg/kg, respectively. The pesticide residues found in citrus fruits were blow maximum residue limits (MRLs) and are not a serious public health problem.

국내 유통되는 감귤류 총 100건을 대상으로 LC-MS을 이용하여 감귤류에 대한 수확 후 처리 농약 잔류량을 조사하였다. 보다 효율적인 농약 분석을 위해 LC-MS를 이용하여 직선성, 정확성, 정밀성, 검출한계 및 정량한계로 분석법의 유효성 검증을 하였다. 상관계수($R^2$)는 0.999이상의 우수한 직선성을 보였고, 농약의 회수율은 80.2-98.3%였고, RSD는 0.48-3.93%로 우수한 재현성을 나타냈다. 검출한계는 0.002-0.008 mg/kg였고, 정량한계는 0.006-0.027 mg/kg였다. 확립된 분석법에 의해 분석한 결과 최고 잔류 수준은 carbendazim은 0.541 mg/kg, thiabendazole은 0.958 mg/kg, imazalil은 0.721 mg/kg, azoxystrobin은 0.052 mg/kg였다. 본 연구결과에서 나타난 수확 후 처리 살균제는 잔류허용기준(MRL) 이내에서 안전하게 관리되고 있으나, 농약의 잔류성을 줄이기 위한 지속적인 모니터링이 필요하다.

Keywords

References

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