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Effects of the 461-nm LED Light and Combination with Acid Stress Treatment on Staphylococcus aureus and Escherichia coli

461-nm LED조사와 산의 병행처리가 Staphylococcus aureus와 Escherichia coli 생육에 미치는 영향

  • Kim, Se-Hun (Department of Food and Nutrition, Yeungnam University) ;
  • Bang, Woo-Suk (Department of Food and Nutrition, Yeungnam University)
  • 김세훈 (영남대학교 식품영양학과) ;
  • 방우석 (영남대학교 식품영양학과)
  • Received : 2013.05.07
  • Accepted : 2013.05.28
  • Published : 2013.08.31

Abstract

This study was conducted to evaluate the disinfection effects of Staphylococcus aureus, and Escherichia coli treated with 461-nm LED and pH 5 at $15^{\circ}C$ for 10 h. S. aureus strains were decreased by about 4 log CFU/mL after 461-nm LED irradiation treatment alone for 10 h. E. coli strains were inactivated by irradiation. However, when microorganisms were subjected to a combined treatment of 461-nm LED and pH 5, both strains were inactivated by irradiation for 7 h. The highest D-value was 5.05 h for S. aureus ATCC 27664 and the lowest D-value was 1.39 h for E.coli O157: H7 ATCC 35150 (p<0.05) with 461-nm LED irradiation. For the combined treatment (461-nm LED and pH 5), the highest D-value was 1.58 h for S. aureus ATCC 19095, whereas the lowest D-value was 0.83 h for S. aureus ATCC 27664 (p<0.05). These data showed that bactericidal effects of a combination of pH 5 with 461-nm LED irradiation were enhanced compared to 461-nm LED irradiation alone.

이 연구는 $15^{\circ}C$에서 10시간동안 pH 5 환경과 461-nm LED를 조사하였을 때 S. aurues (MRSA 포함), E. coli (E.coli O157:H7 포함)의 살균 효과를 측정하고자 수행하게 되었다. 461-nm LED만을 10시간동안 조사하였을 때. S. aureus (MRSA 포함)은 4 log CFU/mL 감소하였다. 그리고 E. coli (E. coli O157:H7 포함)는 9시간동안 조사하였을 때 불활성화되었다. 그러나 pH 5와 461-nm LED를 함께 조사한 결과, 모든 균주들은 7시간동안 조사하였을 때 불활성화되었다. 이 가시광선 불활성화 결과는 외인성의 광감각제없이 이루어 졌다. 461-nm LED만을 조사하였을 때, 가장 높은 D값은 S. aureus (ATCC 27664)으로 5.05시간이었으며 가장 낮은 D값은 E. coli O157: H7 (ATCC 35150)으로 1.39시간이었다. pH 5와 461-nm LED를 함께 조사한 결과, 가장 높은 D값은 S. aureus (ATCC 19095)의 1.58시간이었으며, 가장 낮은 D값은 S. aureus (ATCC 27664)의 0.83시간이었다. 본 연구 결과는 pH 5와 461-nm LED를 함께 조사한 것이 461 nm LED 한가지 환경보다 살균효과가 증가되었다는 것을 보여준다.

Keywords

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