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Sterilization effect of electrolyzed water and chlorine dioxide on Rubus coreanus Miquel

전기분해수와 이산화염소수 처리 복분자(Rubus coreanus Miquel)의 살균효과

  • Teng, Hui (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, You Ho (Post-Harvest Engineering Division, National Academy of Agricultural Science, Rural Development Administrations) ;
  • Lee, Won Young (School of Food Science and Biotechnology, Kyungpook National University)
  • 텅후이 (경북대학교 식품공학부) ;
  • 김유호 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 이원영 (경북대학교 식품공학부)
  • Received : 2013.06.25
  • Accepted : 2013.08.19
  • Published : 2013.08.30

Abstract

This study was carried out in order to investigate sterilization effect and to extend storage periods of the Rubus coreanus by treating with tap water (TW), electrolyzed water (EW) and aqueous chlorine dioxide ($ClO_2$). After each treatment plot was soaked with 10, 50, 100, 200 ppm in each sterilizing solution within 30 sec, each treatment was compared during the storage time at room temperature and refrigerator temperature. As results of total plate count according to temperatures and periods, the microbial sterilizing power of each treatment plot was bigger at EW and $ClO_2$ treatment plots than the TW treatment plot; however, it sharply increased on the high concentration $ClO_2$ treatment plot. Futhermore, the cold storage treatment plot had more outstanding microbial sterilizing power than the room temperature treatment plot. As a result of observing the surface of the Rubus coreanus using scanning electron microscope (SEM), no microbe was seen in EW and $ClO_2$ treatment plot. The results of measuring enzyme activity showed a more significant decrease in EW and $ClO_2$ solutions treatment plot than TW treatment plot but gradually increased with time. The contents of total polyphenol revealed similar values on each treatment. The EW and $ClO_2$ treatment of the Rubus coreanus could be considered as good methods for inhibiting microbial growth in fresh vegetables and fruit, thereby contributing to quality maintenance.

본 연구에서는 수도수, 전기분해수, 이산화염소수 처리에 의한 복분자 딸기의 저장온도 및 기간에 따른 품질 변화를 분석하였다. 복분자 딸기를 수도수, 전기분해수와 이산화염소수 농도별 10, 50, 100, 200 ppm으로 각각 30초 동안 침지를 한 후, 상온과 냉장 저장하여 시간에 따른 저장성 효과를 비교하였다. 또한, 외관 품질유지와 갈변저해효과를 탐색하였다. 또한, 복분자로부터 Bacillus cereus와 Micrococcus luteus의 위해미생물을 분리 동정하였다. 살균세척수를 처리한 후 저장 온도별 시간에 따른 미생물 총균수를 측정한 결과, 각 처리구의 미생물 살균력은 전기분해수와 이산화염소수가 수도수 처리보다 증가하였으며 이산화염소수는 고농도일수록 현저하게 증가하였다. 상온에서 저장하는 구보다 냉장 저장한 구에서의 미생물 억제효과가 뛰어났다. 복분자 딸기의 표면에 주사전자현미경으로 관찰한 결과, 전기분해수와 이산화염소수 처리구에서는 미생물이 생존하지 않았다. 효소활성을 측정한 결과, 수도수 처리구보다 살균세척수 처리구에서 함량이 낮아졌으며 시간이 지남에 따라 서서히 증가하였다. 총 폴리페놀 함량은 세척 방법 간 유사한 결과를 나타내었다. 저장일수가 증가할수록 감소하였고 전기분해수와 이산화염소수 처리구의 함량이 수도수 처리보다는 낮았다. 따라서, 복분자 딸기의 전기분해수와 이산화염소수 처리는 미생물 생육을 억제하면서 신선도를 유지하고 품질 유지 및 저장성 향상에 기여할 것으로 사료된다.

Keywords

References

  1. Marques-Vidal P, Ravasco P, Camilo M (2006) Food stuffs and colorectal cancer risk: a review. Clin Nutr, 25, 14-36 https://doi.org/10.1016/j.clnu.2005.09.008
  2. Kim HC, Lee SI (1991) A comparative study on the health effect of Rubus coreanum. J Herbol, 6, 3-11
  3. Kim MJ, Lee U, Kim SH, Chung HG (2002) Variation of leaf, fruiting and fruit characteristics in Rubus coreanus Miq. Korean J Breed, 34, 50-56
  4. Choi MR, Oh SW, Lee SY (2008) Efficacy of chemical sanitizer in reducing levels of foodborne pathogens and formation of chemically injured cells an cabbage. J Korean Soc Food Sci Nutr, 37, 1337-1342 https://doi.org/10.3746/jkfn.2008.37.10.1337
  5. Daniel L, Yanyun Z (2007) Innovations in the development and application of edible coating for fresh and minimally processed fruits and vegetables. Compr Rev Food Sci F, 6, 60-75 https://doi.org/10.1111/j.1541-4337.2007.00018.x
  6. Shigenobu K, Seiichiro I (2007) Microbial control of fresh produce using electrolyzed water. Jpn Agr Res, 41, 273-282 https://doi.org/10.6090/jarq.41.273
  7. Jung SW, Park KJ, Park KJ, Park BI, Kim YH (1996) Surface sterilization effect of electrolyzed acid water on vegetable. Korean J Food Sci Technol, 28, 1045-1051
  8. Kim YG, Kim TW, Ding T, Oh DH (2009) Effect of electrolyzed water and citric acid on quality enhancement and microbial inhibition in head lettuce. Korean J Food Sci Technol, 41, 578-586
  9. Park SS, Sung JM, Jeong JW, Park KJ, Lim JH (2012) Efficacy of electrolyzed water and aqueous chlorine dioxide for reducing pathogenic microorganism on chinese cabbage. Korean J Food Sci Technol, 44, 240-246 https://doi.org/10.9721/KJFST.2012.44.2.240
  10. Yoo JY, Jang KI (2011) Changes in Quality of Soybean Sprouts Washed with Electrolyzed Water during Storage. J Korean Soc Food Sci Nutr, 40, 586-592 https://doi.org/10.3746/jkfn.2011.40.4.586
  11. Koseki S, Yosida K, Kamitani Y, Itoh K (2004) Efficacy of acidic electrolyzed water for microbial decontamination of cucumbers and strawberries. J Food Protect, 66, 1247-1251
  12. Koseki S, Yoshida K, Isobe S, Itoh K (2001) Decontamination of lettuce using acidic electrolyzed water. J Food Protect, 64, 652-658 https://doi.org/10.4315/0362-028X-64.5.652
  13. Park CM, Hung YC, Doyle MP, Ezeke GOI, Kim C (2001) Pathogen reduction and quality of lettuce treated with electrolyzed water. J Food Sci, 66, 1368-1372 https://doi.org/10.1111/j.1365-2621.2001.tb15216.x
  14. Beuchat LR, Pettigre CA, Tremblay ME, Roselle BJ, Scoute AJ (2005) Lethality of chlorine, chlorine dioxide, and a commercial fruit and vegetable sanitizer to vegetative cells and spores of Bacillus cereus and spores of Bacillus thurigiensis. J Ind Microbiol Biotechnol, 32, 301-308 https://doi.org/10.1007/s10295-005-0212-7
  15. Chang DS, Kim YM, Kim YG (1979) Bacteriological study on cultured vegetables. Bull Kor Fish Soc, 12, 261-266
  16. Materon LA (2003) Survival of Escherichia coli O157:H7 applied to cantaloupes and the effectiveness of chlorinated water and lactic acid as disinfectants. World J Microbiol Biotech, 19, 867-873 https://doi.org/10.1023/A:1026067405248
  17. Park KJ, Lim JH, Kim BK, Kim JC, Jeong JW, Jeong SW (2008) Effect of aqueous chlorine dioxide and citric acid on reduction of Salmonella typhimurium on sprouting radish seeds. Korean J Food Preserv, 15, 754-759
  18. Ryu SH (2007) Effects of aqueous chlorine dioxide against Escherichia coli O157:H7 and Listeria monocytogenes on broccoli served in foodservice institutions. J Korean Soc Food Sci Nutr, 36, 1622-1627 https://doi.org/10.3746/jkfn.2007.36.12.1622
  19. Aebi H (1974) Catalase. In: Bergmeyer HU, ed. Methods of Enzymatic Analysis, Verlag Chemie-Academic Press, New York, p 673-684
  20. Kar M, Mishra D (1976) Catalase, peroxidase and polyphenoloxidase activities during rice leaf senescence. Plant Physiol, 57, 315-319 https://doi.org/10.1104/pp.57.2.315
  21. Kumazawa S, Hamasaka T, Nakayama T (2004) Antioxidant activity of propolis of various geographic origins. Food Chem, 84, 329-339 https://doi.org/10.1016/S0308-8146(03)00216-4
  22. Izumi H (1999) Electrolyzed water as a disinfectant for fresh-cut vegetable. J Food Sci, 64, 536-539 https://doi.org/10.1111/j.1365-2621.1999.tb15079.x
  23. Chang TW, Han JS, Song OJ, Chung DH, Shin IS (2004) Study on reducing methods of natural food-borne pathogenic microorganism originated from Saengshik. Korean J Food Sci Technol, 36, 1020-1025
  24. Park KJ, Lim JH, Kim JH, Jeong JW, Jo JH, Jeong SW (2007) Reduction of microbial load on radish (Raphanus sativus L.) seeds by aqueous chlorine dioxide and hot water treatments. Korean J Food Preserv, 14, 487-491
  25. Singh N, Singh RK, Bhunia AK (2003) Sequential disinfection of Escherichia coli O157:H7 inoculated alfalfa seeds before an during sprouting using aqueous chlorine dioxide, ozonated water, and thyme essential oil. Lebensm-Wiss U-Technol, 36, 235-243 https://doi.org/10.1016/S0023-6438(02)00224-4
  26. Taormina PJ, Beuchatn LR (1999) Comparison of chemical treatment to eliminate enterohemorrhagic Escherichia coli O157:H7 on alfalfa seeds. J Food Prot, 62, 318-324 https://doi.org/10.4315/0362-028X-62.4.318
  27. Granum PE, Lund T (1997) Bacillus cereus and its food poisoning toxins. FEMS Microbiol Lett, 157, 223-228 https://doi.org/10.1111/j.1574-6968.1997.tb12776.x
  28. Jorge UR, Schafer HW, Zottola EA, Davidson PM (1997) Inhibition of Listeria monocytogenes, Escherichia coli O157:H7, and Micrococcus luteus by Linear Furanocoumarins in a Model Food System. J Food Protect, 60, 1050-1054 https://doi.org/10.4315/0362-028X-60.9.1050
  29. Liu M, Qian B, Zhang H, Deng Y, Shen Y, Ping J, Cao L (2010) Sanitizer treatments alleviate lignification of sliced few-flower wild rice (Zizania latifolia Turcz.). Food Res Int, 43, 2363-2368 https://doi.org/10.1016/j.foodres.2010.09.004
  30. Naczk M, Shahidi F (2003) Phenolic compounds in plant foods: Chemistry and health benefits. Nutraceuticals Food, 8, 200-218 https://doi.org/10.3746/jfn.2003.8.2.200
  31. Lee MW (1995) Phenolic compounds from the leaves of Rubus coreanum. Korean J Pharmacogn, 39, 200-204
  32. Vandekinderen I, Camp JV, Meulenaer BD, Veramme K, Bernaert N, Denon Q, Ragaert P, Devlieghere F (2009) Moderate and high doses of sodium hypochlorite, neutral electrolyzed oxidizing water, peroxyacetic acid, and gaseous chlorine dioxide did Not affect the nutritional and sensory qualities of fresh-cut iceberg lettuce (Lactuca sativa Var. capitata L.) after washing. J Agric Food Chem, 57, 4195-4203 https://doi.org/10.1021/jf803742v

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