DOI QR코드

DOI QR Code

극동산 뱀장어, Anguilla japonica 자성화에 미치는 estradiol-17β 유도 효과

Effects of Estradiol-17β on the Feminization of Japanase Eel, Anguilla japonica

  • 김대중 (국립수산과학원 전략연구단) ;
  • 이배익 (국립수산과학원 전략연구단) ;
  • 김경길 (국립수산과학원 전략연구단) ;
  • 김응오 (국립수산과학원 양식관리과) ;
  • 손맹현 (국립수산과학원 양식관리과) ;
  • 성기백 (국립수산과학원 내수면양식연구센터)
  • 투고 : 2013.07.16
  • 심사 : 2013.08.28
  • 발행 : 2013.08.30

초록

산란유도용 암컷 친어 뱀장어를 확보하기 위한 목적으로 estradiol-$17{\beta}$ (E2)의 적정 투여 시기 및 농도를 조사하였다. 실뱀장어 단계(평균 체중 0.16 g, 평균 전장 6.2 cm)에 있어서 E2함유 배합사료를 5개월간 경구투여에 따른 자성화 유도율을 조사한 결과 대조구(10%)에 비해 E2를 경구투여한 실험구인 저농도 E2 투여구(10 mg/kg diet)에서는 70%, 고농도 E2 투여구(25 mg/kg diet)에서는 90%로 높게 나타났다. 또한 어린 뱀장어 단계(치만 단계; 평균 체중 2.6 g, 평균 전장 13.2 cm)에 있어서도 E2함유 배합사료를 4 개월간 경구투여에 따른 자성화 유도율은 대조구(20%)에 비해 E2 투여 실험구(25 mg/kg diet)에서는 60%로 높게 나타났다. 한편 E2 농도에 따른 자성화 유도율의 두드러진 차이는 없었지만, E2 경구투여 시기에 따른 자성화 유도율은 치만단계 보다 실뱀장어 단계에서 높게 나타났다. 그러나 대조구와 E2 경구투여구에 있어서 성장률의 유의적인 차이는 없었다. 따라서 본 연구에 의해 극동산 뱀장어 치어기에 E2 경구투여로 성제어가 가능하였으며, 자성화 유도율은 E2 농도 보다 투여 시기에 의존하는 것으로 추측되었다.

The effects of oral administration of estradiol-$17{\beta}$ (E2) on glass eels (Body weight: $0.16{\pm}0.05g$, Total length: $6.2{\pm}0.9cm$) and young eels (Body weight: $2.6{\pm}0.6g$, Total length: $13.2{\pm}0.6cm$) on gonadal sex and growth were examined, respectively. Glass eels were fed a diet containing E2 at a dose of 10 mg/kg or 25 mg/kg, respectively, for five months. The female ratio significantly increased in all E2-treated groups (10 mg/kg diet group: 70%; 25 mg/kg diet group: 90%) when compared to the control group (10%). Young eels were fed a diet containing E2 at a dose of 25 mg/kg for four months. The female ratio also significantly increased in the E2-treated groups (60%) compared to the control group (20%). The highest female ratio was observed in the stage of glass eels rather than young eels. In all experiments, however, the growth of eels treated with E2 was similar to that of controls to the end of the experiment. Thus, oral administration of E2 could be a good approach to controlling sex differentiation.

키워드

참고문헌

  1. Adachi, S., Ijiri, S., Kazeto, Y. and Yamauchi, K. 2003. Oogenesis in the Japanese eel Anguilla japonica, pp. 301-329. In: Aida, K., Tsukamoto, K. and Yamauchi, K. (eds.), Eel Biology. Springer-verlag: Tokyo, Japan.
  2. Beullens, K., Eding, E. H., Gilson, P., Ollevier, F., Komen, J. and Richter, C. J. J. 1997. Gonadal differentiation, intersexuality and sex ratios of European eel (Anguilla Anguilla L.) maintained in captivity. Aquaculture 153, 135-150. https://doi.org/10.1016/S0044-8486(97)00018-5
  3. Bae, J. Y., Kim, D. J., Lee, J. U., Son, S. G. and Lee, J. K. 2007. Correlationship between artificial maturation season and reproduction coefficient in the cultured eel Anguilla japonica. J Aquaculture 20, 219-225.
  4. Chang, S. U., Kou, G. H. and Liao, I. C. 2004. Temperature adaptation of the Japanese eel (Anguilla japonica) in its early stages. Zool Stud 43, 571-579.
  5. Chiba, H., Iwatsuki, K., Hayami, K. and Yamauchi, K. 1993. Effects of dietary estradiol-17ß on feminization, growth and body composition in the Japanese eel (Anguilla japonica). Comp Biochem Physiol A 106, 367-371. https://doi.org/10.1016/0300-9629(93)90527-B
  6. Colombo, G., Grandi, G. and Rossi, R. 1984. Gonad differentiation and body growth in Anguilla anguilla. J Fish Biol 24, 215-228. https://doi.org/10.1111/j.1095-8649.1984.tb04792.x
  7. Colombo, G. and Grandi, G. 1990. Gonad sex differentiation of Anguilla anguilla by sex steroids. Int Rev Hydrobio 76, 763-773.
  8. Colombo, G. and Grandi, G. 1995. Sex differentiation in the European eel: histological analysis of the effects of sex steroids on the gonad. J Fish Biol 47, 394-413. https://doi.org/10.1111/j.1095-8649.1995.tb01909.x
  9. Devlin, R. H. and Nagahama, Y. 2002. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture 208, 191-364. https://doi.org/10.1016/S0044-8486(02)00057-1
  10. Grandi, G., Poerio, F., Colombo, G. and Chicca, M. 2000. Effects of diet supplementation with carp ovary on gonad differentiation and growth of the European eel. J Fish Biol 57, 1505-1525. https://doi.org/10.1111/j.1095-8649.2000.tb02228.x
  11. Helfman, G. S., Facey, D. E., Hales, L. S. Jr. and Bozeman, E. L. Jr. 1987. Reproductive ecology of the American eel. Am Fish Soc Sym 1, 42-56.
  12. Holmgren, K. and Mosegaard, H. 1996. Implication of individual growth status on the future sex of the European eel. J Fish Biol 49, 910-925. https://doi.org/10.1111/j.1095-8649.1996.tb00089.x
  13. Kagawa, H., Iinuma, N., Tanaka, H., Ohta, H. and Okuzawa, K. 1998. Effects of rearing period in seawater on induced maturation in female Japanese eel Anguilla japonica. Fish Sci 64, 77-82.
  14. Kim, D. J., Kang, E. J., Bae, J. Y., Park, M. W. and Kim, E. O. 2007. Development of the eggs and pre-leptocephalus larvae by natural spawning of artificially-matured Japanase eel, Anguilla japonica. J Aquaculture 20, 160-167.
  15. Kim, D. J., Bae, J. Y. and Kim, E. O. 2007. Changes in sex steroid hormones and ovarian development during artificial maturation of female eel, Anguilla japonica. Integ Biosci 11, 117-124. https://doi.org/10.1080/17386357.2007.9647323
  16. Kitano, T., Takamune, K., Nagahama, Y. and Abe, S. I. 2001. Role of P450 aromatase in gonadal sex differentiation in Japanese flounder (Paralichthys olivaceus). Environ Sci 8, 1-11.
  17. Krueger, W. H. and Oliveira, K. 1999. Evidence for environmental sex determination in the American eel, Anguilla rostrata. Environ Biol Fish 55, 381-389. https://doi.org/10.1023/A:1007575600789
  18. Piferrer, F. 2001. Endocrine sex control strategies for the feminization of teleost fish. Aquaculture 197, 229-281. https://doi.org/10.1016/S0044-8486(01)00589-0
  19. Satoh, H., Nakamura, N. and Hibiya, T. 1962. Studies on the sexual maturation of the eel. I. On the sex differentiation and the maturing process of the gonads. Bull Jpn Soc Sci Fish 28, 579-584. https://doi.org/10.2331/suisan.28.579
  20. Satoh, H., Nimura, Y. and Hibiya, T. 1992. Sex control of the Japanese eel by an estrogen (DES-Na) in feed. Nippon Suisan Gakkai Shi 58, 1211-1218. https://doi.org/10.2331/suisan.58.1211
  21. Tachiki, H., Nakagawa, T., Tamura, K. and Hirose, K. 1997. Effects of oral administration of estradiol-$17{\beta}$ to young on gonadal sex and growth of Japanese eel Anguilla japonica (in Japanese). Suisan Zoshoku 45, 61-66.
  22. Tanaka, H., Kagawa, H. and Ohta, H. 2001. Production of leptocephali of Japanese eel, Anguilla japonica in captivity. Aquaculture 201, 51-60. https://doi.org/10.1016/S0044-8486(01)00553-1
  23. Tanaka, H, Kagawa, H., Ohta, H., Unuma, T. and Nomura, K. 2003. The first production of glass eel in captivity: fish reproductive physiology facilitates great progress in aquaculture. Fish Physiol Biochem 28, 493-497. https://doi.org/10.1023/B:FISH.0000030638.56031.ed
  24. Todd, P. R. 1980. Size and age of migrating New Zealand freshwater eels (Anguilla spp.). New Zealand J Mar Fresh Res 14, 283-293. https://doi.org/10.1080/00288330.1980.9515871
  25. Walsh, C. T., Pease, B. C. and Booth, D. J. 2003. Sexual dimorphism and gonadal development of the Australian longfinned river eel. J Fish Biol 63, 137-152. https://doi.org/10.1046/j.1095-8649.2003.00136.x
  26. Wiberg, U. H. 1983. Sex determination in the European eel (Anguilla Anguilla L.): a hypothesis based on cytogenetic results, correlated with findings of skewed sex ratio. Cytogen Cell Gen 36, 589-598. https://doi.org/10.1159/000131981

피인용 문헌

  1. Effects of Water Temperature and Estradiol-17β on the Sex Ratio and Growth of the Japanese Eel, Anguilla japonica vol.23, pp.12, 2013, https://doi.org/10.5352/JLS.2013.23.12.1454
  2. Effect of Immersion and Oral Vaccination using Formalin-killed Edwardsiella tarda against Eel Anguilla japonica vol.27, pp.3, 2015, https://doi.org/10.13000/JFMSE.2015.27.3.672
  3. Morphological Observation of Pre- and Postovulated Eggs from Artificially Maturated Wild and Feminized Eels vol.26, pp.6, 2014, https://doi.org/10.13000/JFMSE.2014.26.6.1402
  4. Effect of Artificially Sexual Maturation by hCG Dose and Injection Interval in Cultured Male Eel, Anguilla japonica vol.30, pp.5, 2018, https://doi.org/10.13000/JFMSE.2018.10.30.5.1578