Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Anti-Mullerian Hormone Serum Concentrations in Prenatal and Postnatal Period in Murine

  • Kim, Dae Young (Division of Life Science, College of BioNano Technology, Gachon University)
  • Received : 2013.05.01
  • Accepted : 2013.05.24
  • Published : 2013.06.30
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Abstract

Mullerian inhibiting substance (MIS) is a member of the TGF-${\beta}$ (transforming growth factor-${\beta}$) family whose members play key roles in development, suppression of tumour growth, and feedback control of the pituitary-gonadal hormone axis. MIS is expressed in a highly tissue-specific manner in which it is restricted to male Sertoli cells and female granulose cells. The serum levels of MIS in prenatal and postnatal ICR mice were measured using the enzyme-linked immuno-solvent assay (ELISA) using the MIS/AMH antibody. Mice were grouped by age: the significant periods were at the onset of development. During sex organ differentiation, no remarkable difference between female and male foetus MIS serum levels (both<0.1 ng/ml) was observed. However, MIS serum levels in pregnant mice markedly changed (4.5~12.2 ng/ml). After birth, postnatal female and male mice serum MIS levels changed considerably (male: <0.1~138.5 ng/ml, female: 5.3~103.4 ng/ml), and the changing phase were diametrically opposed (male: decreasing, female: fluctuating). These findings suggest that MIS may have strong associations with not only develop-ment but also puberty. For further studies, establishing the standard MIS serum levels is of importance. Our study provides the basic information for the study of MIS interactions with reproductive organ disability, cancer, and the effect of other hormone or menopause. We hypothesise that if MIS is regularly injected into middle-age women, meno-pause will be delayed. We detected that serum MIS concentration curves change with age. The changing phase is different between males and females, and this difference is significant after birth. Moreover, MIS mRNA is expressed during the developmental period (prenatal) and also in the postnatal period. This finding indicates that MIS may play a significant role in the developmental stage and in growth after birth.

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References

  1. Ahima RS, Dushay J, Flier SN, Prabakaran D and Flier JS. 1997. Leptin accelerates the onset of puberty in normal female mice. J. Clin. Invest. 99:391-395. https://doi.org/10.1172/JCI119172
  2. Bedecarrats GY, O'Neill FH, Norwitz ER, Kaiser UB and Teixeira J. 2003. Regulation of gonadotropin gene expression by Mullerian inhibiting substance. Proc. Natl. Acad. Sci. USA 100:9348-9353. https://doi.org/10.1073/pnas.1633592100
  3. Bronson FH. 2001. Puberty in female mice is not associated with increases in either body fat or leptin. Endocrinology 142:4758-4761. https://doi.org/10.1210/endo.142.11.8495
  4. Chris W and Robert A. 2002. Germ Cell. In: Janet R and Patrick PLT (Ed), In Mouse Development: Patterning, Morphogenesis, and Organogenesis, Academic Press., pp. 181-190.
  5. Dawes HE, Berlin DS, Lapidus DM, Nusbaum C, Davis TL and Meyer BJ. 1999. Dosage compensation proteins targeted to X chromosomes by a determinant of hermaphrodite fate. Science 284:1800-1804. https://doi.org/10.1126/science.284.5421.1800
  6. Donahoe PK, Clarke T, Teixeira J, Maheswaran S and Mac- Laughlin DT. 2003. Enhanced purification and production of Mullerian inhibiting substance for therapeutic applications. Mol. Cell. Endocrionol. 211:37-41. https://doi.org/10.1016/j.mce.2003.09.009
  7. Durlinger AL, Gruijters MJ, Kramer P, Karels B, Ingraham HA, Nachtigal MW, Uilenbroek JT, Grootegoed JA and Themmen AP. 2002a. Anti-Mullerian hormone inhibits initiation of primordial follicle growth in the mouse ovary. Endocrinology 143:1076-1084.
  8. Durlinger AL, Gruijters MJ, Kramer P, Karels B, Kumar TR, Matzuk MM, Rose UM, de Jong FH, Uilenbroek JT, Grootegoed JA and Themmen AP. 2001. Anti-Mullerian hormone attenuates the effects of FSH on follicle development in the mouse ovary. Endocrinology 142:4891-4899.
  9. Durlinger AL, Kramer P, Karels B, de Jong FH, Uilenbroek JT, Grootegoed JA and Themmen AP. 1999. Control of primordial follicle recruitment by anti-Mullerian hormone in the mouse ovary. Endocrionology 140:5789-5796.
  10. Durlinger AL, Visser JA and Themmen AP. 2002b. Regulation of ovarian function: the role of anti-Mullerian hormone. Reproduction 124:601-609. https://doi.org/10.1530/rep.0.1240601
  11. Dutertre M, Gouedard L, Xavier F, Long WQ, di Clemente N, Picard JY and Rey R. 2001. Ovarian granulose cell tumors express a functional membrane receptor for anti-Mullerian hormone in transgenic mice. Endocrinology 142:4040-4046.
  12. Fujisawa M, Yamasaki T, Okada H and Kamidono S. 2002. The significance of anti-Mullerian hormone concentration in seminal plasma for spermatogenesis. Hum. Reprod. 17: 968-970. https://doi.org/10.1093/humrep/17.4.968
  13. Hoshiya Y, Gupta V, Segev DL, Hoshiya M, Carey JL, Sasur LM, Tran TT, Ha TU and Maheswaran S. 2003. Mullerian inhibiting substance induces NFkB signaling in breast cancer cells. Mol. Cell. Endocrinol. 211:43-49. https://doi.org/10.1016/j.mce.2003.09.010
  14. Josso N, Belville C, di Clemente N and Picard JY. 2005. AMH and AMH receptor defects in persistent Mullerian duct syndrome. Hum. Reprod. Update 11:351-356. https://doi.org/10.1093/humupd/dmi014
  15. Keene DE, Suescun MO, Bostwick MG, Chandrashekar V, Bartke A and Kopchick JJ. 2002. Puberty is delayed in male growth hormone receptor gene-disrupted mice. J. Androl. 23:661-668.
  16. Kunieda T, Xian M, Kobayashi E, Imamichi T, Moriwaki K and Toyoda Y. 1992. Sexing of mouse preimplantation by detection of Y chromosome specific sequences using polymerase chain reaction. Biol. Reprod. 46:692-697. https://doi.org/10.1095/biolreprod46.4.692
  17. Lukas-Croisier C, Lasala C, Nicaud J, Bedecarras P, Kumar TR, Dutertre M, Matzuk MM, Picard JY, Josso N and Rey R. 2003. Follicle-stimulating hormone increase testicular anti-Mullerian hormone (AMH) production through Sertoli cell proliferation and a nonclassical cyclic adenosine 5' -monophosphate mediated activation of the AMH gene. Mol. Endcrionol. 17:550-561. https://doi.org/10.1210/me.2002-0186
  18. McClive PJ and Sinclair AH. 2001. Rapid DNA extraction and PCR-sexing of mouse embryos. Mol. Reprod. Dev. 60:225-226. https://doi.org/10.1002/mrd.1081
  19. Morrish BC and Sinclair AH. 2002. Vertebrate sex determination: many means to an end. Reproduction 124:447-457. https://doi.org/10.1530/rep.0.1240447
  20. Munsterberg A and Lovell-Badge R. 1991. Expression of the mouse anti-Mullerian hormone gene suggests a role in both male and female sexual differentiation. Development 113: 613-624.
  21. Racine C, Rey R, Forest MG, Louis F, Ferre A, Huhtaniemi I, Josso N and di Clemente N. 1998. Receptors for anti-Mullerian hormone on leydig cells are responsible for its effects on steroidogenesis and cell differentiation. Pro. Natl. Acad. Sci. USA 95:594-599. https://doi.org/10.1073/pnas.95.2.594
  22. Rajpert-De Meyts E, Jorgensen N, Graem N, Muller J, Cate RL and Skakkebaek NE. 1999. Expression of anti-Mullerian hormone during normal and pathological gonadal development: association with differentiation of Sertoli granulose cells. J. Clin. Endocrinol. Metab. 84:3836-3844.
  23. Schmidt KL, Kryger-Baggesen N, Byskov AG and Andersen CY. 2005. Anti-Mullerian hormone initiates growth of human primordial follicles in vitro. Mol. Cell. Endocrinol. 234:87-93. https://doi.org/10.1016/j.mce.2004.12.010
  24. Stubbs SA, Hardy K, Da Silva-Buttkus P, Stark J, Webber LJ, Flanagan AM, Themmen AP, Visser JA, Groome NP and Franks S. 2005. Anti-Mullerian hormone protein expression is reduced during the initial stages of follicle development in human polycystic ovaries. J. Clin. Endocrinol. Metab. 90:5536-5543. https://doi.org/10.1210/jc.2005-0907
  25. Themmen AP. 2005. Anti-Mullerian hormone: its role in follicular growth initiation and survival and as an ovarian reserve marker. J. Natl. Cancer Inst. Monogr. 34:18-21.
  26. van Rooij IA, Broekmans FJ, te Velde ER, Fauser BC, Bancsi LF, de Jong FH and Themmen AP. 2002. Serum anti- Mullerian hormone levels: a novel measure of ovarian reserve. Hum. Reprod. 17:3065-3071. https://doi.org/10.1093/humrep/17.12.3065
  27. Visser JA and Themmen AP. 2005. Anti-Mullerian hormone and folliculogenesis. Mol. Cell. Endocrinol. 234:81-86. https://doi.org/10.1016/j.mce.2004.09.008
  28. Visser JA, de Jong FH, Laven JS and Themmen AP. 2006. Anti-Mullerian hormone: a new marker for ovarian function. Reproduction 131:1-9. https://doi.org/10.1530/rep.1.00529