Asian-Australasian Journal of Animal Sciences

  • 제26권10호
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  • Pages.1365-1373
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  • 2013
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effect of FTO Expression and Polymorphism on Fat Deposition in Suzhong Pigs

  • Fu, Yanfeng (Institute of Animal Science, Jiangsu Academy of Agricultural Sciences) ;
  • Li, Lan (College of Veterinary Medicine, China Agricultural University) ;
  • Ren, Shouwen (Institute of Animal Science, Jiangsu Academy of Agricultural Sciences)
  • 투고 : 2013.01.16
  • 심사 : 2013.05.26
  • 발행 : 2013.10.01
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초록

Fat mass and obesity associated gene (FTO) plays an important role in appetite control and energy consumption in human and mice. In order to examine FTO expression influence on fat deposition in Suzhong pigs, FTO mRNA expression was detected in 16 tissues by RT-PCR, FTO protein expression was detected in 5 tissues by western blot, and association of FTO polymorphism with meat quality traits was analyzed in Suzhong populations with 714 records. RT-PCR results revealed that FTO mRNA was expressed in all sixteen tissues with significant differences (p<0.05), expression in backfat was significantly higher than that of any other tissue (p<0.05), and expression in longissimus dorsi muscle had the second highest significance level (p<0.05). Western blot results demonstrated that FTO protein was highly expressed in backfat and longissimus dorsi muscle. Furthermore, FTO mRNA and protein expression in tissues of high-fat pigs was significantly higher than that of low-fat pigs (p<0.05), suggesting FTO expression had advantageous effects on fat deposition. FTO polymorphism results evidenced that at A227G locus, G allele seemed to have advantageous effects on fat deposition, indicating it could be a significant candidate gene for improving pork quality in Suzhong pigs.

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  3. Differential expression of six genes in fat-type Hungarian Mangalica and other pigs vol.59, pp.2, 2013, https://doi.org/10.5194/aab-59-259-2016
  4. Analysis of FTO and PLIN2 Polymorphisms in Relation to Carcass and Meat Quality Traits in Pigs vol.19, pp.1, 2019, https://doi.org/10.2478/aoas-2018-0053
  5. Excessive BCAA regulates fat metabolism partially through the modification of m 6 A RNA methylation in weanling piglets vol.17, pp.1, 2013, https://doi.org/10.1186/s12986-019-0424-x