The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Responses of Guava Plants to Inoculation with Arbuscular Mycorrhizal Fungi in Soil Infested with Meloidogyne enterolobii

  • Received : 2012.10.16
  • Accepted : 2013.04.12
  • Published : 2013.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

In the Northeast of Brazil, expansion of guava crops has been impaired by Meloidogyne enterolobii that causes root galls, leaf fall and plant death. Considering the fact that arbuscular mycorrhizal Fungi (AMF) improve plant growth giving protection against damages by plant pathogens, this work was carried out to select AMF efficient to increase production of guava seedlings and their tolerance to M. enterolobii. Seedlings of guava were inoculated with 200 spores of Gigaspora albida, Glomus etunicatum or Acaulospora longula and 55 days later with 4,000 eggs of M. enterolobii. The interactions between the AMF and M. enterolobii were assessed by measuring leaf number, aerial dry biomass, $CO_2$ evolution and arbuscular and total mycorrhizal colonization. In general, plant growth was improved by the treatments with A. longula or with G. albida. The presence of the nematode decreased arbuscular colonization and increased general enzymatic activity. Higher dehydrogenase activity occurred with the A. longula treatment and $CO_2$ evolution was higher in the control with the nematode. More spores and higher production of glomalin-related soil proteins were observed in the treatment with G. albida. The numbers of galls, egg masses and eggs were reduced in the presence of A. longula. Inoculation with this fungus benefitted plant growth and decreased nematode reproduction.

Keywords

References

  1. Affokpon, A., Coyne, D. L., Lawouin, L., Tossou, C., Agbed, R. D. and Coosemans, J. 2011. Effectiveness of native West African arbuscular mycorrhizal fungi in protecting vegetable crops against root- knot nematode. Biol. Fertil. Soils 47:207-217. https://doi.org/10.1007/s00374-010-0525-1
  2. Anjos, E. C. T., Cavalcante, U. M. T., Goncalves, D. M. C., Pedrosa, E. M. R., Santos, V. F. and Maia, L. C. 2010. Interactions between an arbuscular mycorrhizal fungus (Scutellospora heterogama) and the root-knot nematode (Meloidogyne incognita) on sweet passion fruit (Passiflora alata). Braz. Arch. Biol. Technol. 53:801-809. https://doi.org/10.1590/S1516-89132010000400008
  3. Azcon-Aguilar, C. and Barea, J. M. 1996. Arbuscular mycorrhizas and biological control of soil-borne plant pathogens - an overview of the mechanisms involved. Mycorrhiza 6:457-464.
  4. Bedini, S., Avio, L., Argese, E. and Giovannetti, M. 2007. Effects of long-term land use on arbuscular mycorrhizal fungi and glomalin-related soil protein. Agr. Ecosyst. Environ. 120:463-466. https://doi.org/10.1016/j.agee.2006.09.010
  5. Borowicz, V. A. 2001. Do arbuscular mycorrhizal fungi alter plant-pathogen relations- Ecology 82:3057-3068.
  6. Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the prin ciple of protein-dye binding. Anal. Biochem. 72:248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  7. Brundrett, M. C., Pich, Y. and Peterson, R. L. 1984. A new method for observing the morphology of vesicular-arbuscular mycorrhizae. Can. J. Bot. 62:2128-2134. https://doi.org/10.1139/b84-290
  8. Carling, D. E., Roncadori, R. W. and Hussey, R. S. 1996. Interactions of arbuscular mycorrhizae Meloidogyne arenaria, and phosphorus fertilization on peanut. Mycorrhiza 6:9-13.
  9. Carneiro, R. M. D. G., Moreira, W. A., Almeida, M. R. A. and Gomes, A. C. M. M. 2001. Primeiro registro de Meloidogyne mayaguensis em goiabeira no Brasil. Nematol. Bras. 25:223-228.
  10. Carneiro, R. G., Mazzafera, P., Ferraz, L. C. C. B., Muraoka, T. and Trivelin, P. C. O. 2002. Uptake and translocation of nitrogen, phosphorus and calcium in soybean infected with Meloidogyne incognita and M. javanica. Fitopatol. Bras. 27: 141-150. https://doi.org/10.1590/S0100-41582002000200004
  11. Casida, L. E., Klein, D. and Santoro, T. 1964. Soil dehydrogenase. Soil Sci. 98:371-376. https://doi.org/10.1097/00010694-196412000-00004
  12. Castillo, P., Nico, A. I., Azcon-Aguilar, C., del Rio Rincon, C., Calvet, C. and Jimenez-Diaz, R. M. 2006. Protection of olive planting stocks against parasitism of root-knot nematodes by arbuscular mycorrhizal fungi. Plant Pathol. 55:705-713. https://doi.org/10.1111/j.1365-3059.2006.01400.x
  13. Cofcewicz, E. T., Medeiro, C. A. B., Carneiro, R. M. D. G. and Pierobom, C. R. 2001. Interacao dos fungos micorrizicos arbusculares Glomus etunicatum e Gigaspora margarita e o nematoide das galhas Meloidogyne javanica em tomateiro. Fitopatol. Bras. 26:65-70. https://doi.org/10.1590/S0100-41582001000100011
  14. Costa, C. M. C., Maia, L. C., Cavalcante, U. M. T. and Nogueira, R. J. M. C. 2001. Influencia de fungos micorrizicos arbusculares sobre o crescimento de dois genotipos de aceroleira (Malpighia emarginata D. C.). Pesqui. Agropecu. Bras. 36:893-901. https://doi.org/10.1590/S0100-204X2001000600007
  15. Diedhiou, P. M., Hallmann, J., Oerke, E. C. and Dehne, H. W. 2003. Effects of arbuscular mycorrhizal fungi and a nonpathogenic Fusarium oxysporum on Meloidoyne incognita infestation of tomato. Mycorrhiza 13:199-204. https://doi.org/10.1007/s00572-002-0215-4
  16. Elsen, A., Baimey, H., Swennen, R. and De Waele, D. 2003. Relative mycorrhizal dependency and mycorrhiza-nematode interaction in banana cultivars (Musa spp.) differing in nematode susceptibility. Plant Soil 256:303-313. https://doi.org/10.1023/A:1026150917522
  17. Fernandes, S. A. P., Bettiol, W. and Cerri, C. C. 2005. Effect of sewage sludge on microbial biomass, basal respiration, metabolic quotient and soil enzymatic activity. Appl. Soil Ecol. 30:65-77. https://doi.org/10.1016/j.apsoil.2004.03.008
  18. Gerdemann, J. W. and Nicolson, T. H. 1963. Spores of mycorrizal endogone species extracted from soil by wet sieving and decanting. T. Brit. Mycol. Soc. 46:235-244. https://doi.org/10.1016/S0007-1536(63)80079-0
  19. Gianfreda, L., Rao, M. A., Piotrowska, A., Palumbo, G. and Colombo, C. 2005. Soil enzyme activities as affected by anthropogenic alterations: intensive agricultural practices and organic pollution. Sci. Total Environ. 341:265-279. https://doi.org/10.1016/j.scitotenv.2004.10.005
  20. Grisi, B. M. 1978. Metodo quimico de medicao da respiracao edafica: alguns aspectos tecnicos. Cien. Cul. 30:82-88.
  21. Habte, M., Zhang, Y. C. and Schmitt, D. P. 1999. Effectiveness of Glomus species in protecting white clover against nematode damage. Can. J. Bot. 77:135-139.
  22. Hol, W. H. G. and Cook, R. 2005. An overview of arbuscular mycorrhizal fungi-nematode interactions. Basic Appl. Ecol. 6:489-503. https://doi.org/10.1016/j.baae.2005.04.001
  23. Hussey, R. S. and Barker, K. R. 1973. A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Dis. Rep. 57:1025-1028.
  24. Jaizme-Vega, M. C., Tenoury, P., Pinochet, J. and Jaumot, M. 1997. Interactions between the root-knot nematode Meloidogyne incognita and Glomus mosseae in banana. Plant Soil 196:27-35. https://doi.org/10.1023/A:1004236310644
  25. Jaizme-Vega, M. C., Rodriguez- Romero, A. S. and Nunez, L. A. B. 2006. Effect of the combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria on papaya (Carica papaya L.) infected with the root-knot nematode Meloidogyne incognita. Fruits 61:151-162. https://doi.org/10.1051/fruits:2006013
  26. Jenkins, W. R. 1964. A rapid centrifugal flotation technique for separating nematodes from soil. Plant Dis. Rep. 48:692.
  27. Maia, L. C., Silveira, N. S. S. and Cavalcante, U. M. T. 2006. Interaction between arbuscular mycorrhizal fungi and root pathogens. In: Handbook of Microbial Biofertilizers, ed. by M. K. Rai, pp. 325-351. New Delhi: The Haworth Press.
  28. McGonigle, T. P., Miller, M. H., Evans, D. G., Fairchild, G. L. and Swan, J. A. 1990. A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol. 115:495-501. https://doi.org/10.1111/j.1469-8137.1990.tb00476.x
  29. Schiavo, J. A. and Martins, M. A. 2002. Producao de mudas de goiabeira (Psidium guajava L.) inoculadas com o fungo micorrizico arbuscular Glomus clarum, em substrato agroindustrial. Rev. Bras. Frutic. 24:519-523. https://doi.org/10.1590/S0100-29452002000200048
  30. Siddiqui, Z. A. and Mahmood, I. 1995. Role of plant symbionts in nematode management: a review. Bioresource Technol. 54: 217-226. https://doi.org/10.1016/0960-8524(95)00137-9
  31. Siddiqui, Z. A. and Mahmood, I. 1998. Effect of a plant growth promoting bacterium, an AM fungus and soil types on the morphometrics and reproduction of Meloidogyne javanica on tomato. Appl. Soil Ecol. 8:77-84. https://doi.org/10.1016/S0929-1393(97)00061-9
  32. Silva, M. A., Cavalcante, U. M. T., Silva, F. S. B., Soares, S. A. G. and Maia, L. C. 2004. Crescimento de mudas de maracujazeiro- doce (Passiflora alata Curtis) associadas a fungos micorrizicos arbusculares (Glomeromycota). Acta Bot. Bras. 18:981-985. https://doi.org/10.1590/S0102-33062004000400028
  33. Silva, M. A. 2005. Aplicacao de fungos micorrizicos arbusculares (FMA) na aclimatizacao de duas plantas ornamentais tropicais micropropagadas, visando tolerancia ao parasitismo de Meloidogyne arenaria. Dissertacao. Universidade Federal de Pernambuco, Brasil.
  34. Smith, S. E., Facelli, E., Pope, S. and Smith, F. A. 2009. Plant performance in stressful environments: interpreting new and established knowledge of the roles of arbuscular mycorrhizas. Plant Soil 326:3-20.
  35. Statsoft. 1997. Statistica for Windows. Tulsa (CD-ROM).
  36. Swisher, R. and Carrol, G. C. 1982. Fluorescein diacetate hydrolysisas an estimator of microbial biomass on coniferous needle surfaces. Microbial Ecol. 6:217-226.
  37. Taylor, J. and Harrier, L. A. 2001. A comparison of development and mineral nutrition of micropropagated Fragaria ${\times}$ anan assa cv. Elvira (strawberry) when colonized by nine species of arbuscular mycorrhizal fungi. Appl. Soil Ecol. 18:205-215. https://doi.org/10.1016/S0929-1393(01)00164-0
  38. Tu, C., Koenning, S. R. and Hu, S. 2003. Root-parasitic nematodes enhance soil microbial activities and nitrogen mineralization. Microbial Ecol. 46:134-144. https://doi.org/10.1007/s00248-002-1068-2
  39. Vos, C., Geerinckx, K., Mkandawire, R., Panis, B., De Waele, D. and Elsen, A. 2012. Arbuscular mycorrhizal fungi affect both penetration and further life stage development of root-knot nematodes in tomato. Mycorrhiza 22:157-163. https://doi.org/10.1007/s00572-011-0422-y
  40. Wright, S. F. and Upadhyaya. 1996. A Extraction of an abundant and unusual protein from soil and comparison on hyphal protein of arbuscular mycorrhizal fungi. Soil Sci. 161:575-586. https://doi.org/10.1097/00010694-199609000-00003
  41. Zhang, L., Zhang, J., Christie, P. and Li, X. 2008. Pre-inoculation with arbuscular mycorrhizal fungi suppresses root knot nematode (Meloidogyne incognita) on cucumber (Cucumis sativus). Biol. Fert. Soil 45:205-211. https://doi.org/10.1007/s00374-008-0329-8

Cited by

  1. Management of the biological diversity of AM fungi by combination of host plant succession and integrity of extraradical mycelium vol.112, 2017, https://doi.org/10.1016/j.soilbio.2017.05.018
  2. Effect of Arbuscular Mycorrhizal Fungi on Pratylenchus penetrans Infestation in Apple Seedlings under Greenhouse Conditions vol.7, pp.4, 2018, https://doi.org/10.3390/pathogens7040076