The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Reverse Transcription Polymerase Chain Reaction-based System for Simultaneous Detection of Multiple Lily-infecting Viruses

  • Kwon, Ji Yeon (Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University) ;
  • Ryu, Ki Hyun (Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University) ;
  • Choi, Sun Hee (Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University)
  • Received : 2013.04.14
  • Accepted : 2013.05.13
  • Published : 2013.09.01
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Abstract

A detection system based on a multiplex reverse transcription (RT) polymerase chain reaction (PCR) was developed to simultaneously identify multiple viruses in the lily plant. The most common viruses infecting lily plants are the cucumber mosaic virus (CMV), lily mottle virus (LMoV), lily symptomless virus (LSV). Leaf samples were collected at lily-cultivation facilities located in the Kangwon province of Korea and used to evaluate the detection system. Simplex and multiplex RT-PCR were performed using virus-specific primers to detect single- or mixed viral infections in lily plants. Our results demonstrate the selective detection of 3 different viruses (CMV, LMoV and LSV) by using specific primers as well as the potential of simultaneously detecting 2 or 3 different viruses in lily plants with mixed infections. Three sets of primers for each target virus, and one set of internal control primers were used to evaluate the detection system for efficiency, reliability, and reproducibility.

Keywords

References

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