Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Effects of Eucommiae Cortex Pharmacopuncture Injected at Sinsu($BL_{23}$) on Neuropathic Pain in Rats

신유(腎兪)($BL_{23}$) 두중약침(杜仲藥鍼)이 흰쥐의 신경병리성 동통모델에 미치는 영향

  • Lee, Dong Geun (Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Lee, Ook Jae (Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Lee, Ju Hee (Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Lee, Sang Hyun (Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Lee, Jung Hun (Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Shin, Jeong Cheol (Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Kim, Jae Hong (Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University)
  • 이동근 (동신대학교 한의과대학 침구의학교실) ;
  • 이욱재 (동신대학교 한의과대학 침구의학교실) ;
  • 이주희 (동신대학교 한의과대학 침구의학교실) ;
  • 이상현 (동신대학교 한의과대학 침구의학교실) ;
  • 이정훈 (동신대학교 한의과대학 침구의학교실) ;
  • 신정철 (동신대학교 한의과대학 침구의학교실) ;
  • 김재홍 (동신대학교 한의과대학 침구의학교실)
  • Received : 2013.08.06
  • Accepted : 2013.08.26
  • Published : 2013.09.20
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Abstract

Objectives : The purpose of this study is to examine whether Eucommiae Cortex pharmacopuncture may affect to the neuropathic pain in a rat model. Methods : To produce the model of neuropathic pain, under isoflurane 2.5 % anesthesia, underwent tight ligation by 6.0 silk thread and transection of the tibial and sural nerves, leaving the common peroneal nerve intact. After neuropathic surgery, the author examined if the exhibited the behavioral sign of allodynia. The allodynia was assessed by stimulating the plantar with Dynamic Plantar Aesthesiometer. Three days after the neuropathic surgery, Eucommiae Cortex pharmacopuncture was injected at Sinsu($BL_{23}$) once every week for 6 weeks. After that, the author examined the withdrawal response of neuropathic rats' leg by Dynamic Plantar Aesthesiometer. And also the author examined Bax, Bcl-2, Bax/Bcl-2 ratio in the spinal cord of neuropathic rats and the change of WBC, RBC, HGB, HCT count in the blood of neuropathic rats. Results : 1. The Eucommiae Cortex pharmacopuncture decreased the withdrawal response of mechanical allodynia that assessed with Dynamic Plantar Aesthesiometer in EC2-$BL_{23}$ group as compared with control group. 2. The Eucommiae Cortex pharmacopuncture decreased Bax/Bcl-2 ratio in EC1-$BL_{23}$, EC2-$BL_{23}$ group. But The Eucommiae Cortex pharmacopuncture injected at Sinsu($BL_{23}$) didn't change Bax, Bcl-2 expression level in the all group. 3. The Eucommiae Cortex pharmacopuncture decreased WBC count in EC1-$BL_{23}$, EC2-$BL_{23}$ group. Conclusions : We have noticed that Eucommiae Cortex pharmacopuncture decreased mechanical allodynia in the model of neuropathic pain compared with the control group. Bax/Bcl-2 ratio in spinal cord of that group was also decreased compared with the control group. This study can be used as a basic resource on a study and a treatment of neuropathic pain.

Keywords

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